ANKZF1 (NM_018089) Human Tagged ORF Clone
SKU
RC201054
ANKZF1 (Myc-DDK-tagged)-Human ankyrin repeat and zinc finger domain containing 1 (ANKZF1), transcript variant 1
$665.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | ANKZF1 |
| Synonyms | Vms1; ZNF744 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_018089 |
| ORF Size | 2178 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_018089.3 |
| RefSeq Size | 2594 bp |
| RefSeq ORF | 2181 bp |
| Locus ID | 55139 |
| UniProt ID | Q9H8Y5 |
| Cytogenetics | 2q35 |
| MW | 80.9 kDa |
| Summary | Plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress (PubMed:28302725). Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_018089" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201054L3 | Lenti ORF clone of Human ankyrin repeat and zinc finger domain containing 1 (ANKZF1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$965.00
|
|
| RC201054L4 | Lenti ORF clone of Human ankyrin repeat and zinc finger domain containing 1 (ANKZF1), transcript variant 1, mGFP tagged | 10 ug |
$965.00
|
|
| RG201054 | ANKZF1 (tGFP-tagged) - Human ankyrin repeat and zinc finger domain containing 1 (ANKZF1), transcript variant 1 | 10 ug |
$865.00
|
|
| SC318211 | ANKZF1 (untagged)-Human ankyrin repeat and zinc finger domain containing 1 (ANKZF1), transcript variant 1 | 10 ug |
$732.00
|
|
| SC319498 | ANKZF1 (untagged)-Human ankyrin repeat and zinc finger domain containing 1 (ANKZF1), transcript variant 1 | 10 ug |
$665.00
|
|
Citations
Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats
Tseng, Krans, Malik et al
Nucleic Acids Research (2024) 52 (10), 5928-5949 DOI: 10.1093/nar/gkae137
Tseng, Krans, Malik et al
Nucleic Acids Research (2024) 52 (10), 5928-5949 DOI: 10.1093/nar/gkae137
Cited by 25
AI Summary: OriGene contributed the pCMV6-hANKZF1-Myc-FLAG plasmid (RC201054) to this study, which was used to investigate the role of the ANKZF1 ribosomal quality control factor in regulating repeat-associated non-AUG (RAN) translation from GC-rich repeats.
Snippet: pBI-dsRED empty vector was cloned using annealing primers flanked by NheI and EcoRV. pCMV3TAG8li-hRNF160-3FLAG was a gift from Martin Dorf (Addgene plasmid #159138). pCMV6-hANKZF1-Myc-FLAG was purchased from Origene, RC201054. All other reporter sequences have been previously published (44,49)
Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats
Tseng, Malik, Deng et al
bioRxiv (2023)
Tseng, Malik, Deng et al
bioRxiv (2023)
Cited by 4
Snippet: empty vector was cloned using annealing primers flanked by NheI and EcoRV. pCMV3TAG8li-hRNF160–3FLAG was a gift from Martin Dorf (Addgene plasmid #159138). pCMV6-hANKZF1-Myc-FLAG was purchased from Origene, RC201054. All other reporter sequences have been previously published (44, 49). RNA T7 synthesis pcDNA3.1(+) plasmids containing nano-luciferase and 3xFLAG (NLuc-3xFLAG) reporters were linea
MISTERMINATE Mechanistically Links Mitochondrial Dysfunction with Proteostasis Failure
Wu, Tantray, Lim et al
Mol Cell (2019) 75 (4), 835-848.e8 DOI: 10.1016/j.molcel.2019.06.031
Wu, Tantray, Lim et al
Mol Cell (2019) 75 (4), 835-848.e8 DOI: 10.1016/j.molcel.2019.06.031
Cited by 112
AI Summary: OriGene products, specifically the C-I30 antibody (Cat# TA500053) and the NDUFS3 antibody (Cat# TA501481), were used in this study to detect and analyze the levels and modifications of mitochondrial complex I subunits, thereby helping to elucidate the molecular mechanisms linking mitochondrial dysfunction to proteostasis failure.
MISTERMINATE Mechanistically Links Mitochondrial Dysfunction with Proteostasis Failure
Wu, Tantray, Lim et al
Molecular Cell (2019) 75 (4), 835-848.e8 DOI: 10.1016/j.molcel.2019.06.031
Wu, Tantray, Lim et al
Molecular Cell (2019) 75 (4), 835-848.e8 DOI: 10.1016/j.molcel.2019.06.031
AI Summary: OriGene products pCMV6-FLAG-NOT4 (cat#: RC217418) and pCMV6-ANKZF1 (cat#: RC201054 TrueORF) were used as expression plasmids to investigate the molecular mechanisms linking mitochondrial dysfunction and proteostasis failure in this study.
Snippet: … MISTERMINATE Mechanistically Links Mitochondrial Dysfunction with Proteostasis Failure Zhihao Wu1, Ishaq Tantray1, Junghyun Lim1,2, Songjie Chen3, Yu Li1, Zoe Davis4, Cole Sitron4, Jason Dong1, Suzana Gispert5 View as HTML;pCMV6-FLAG-NOT4 and pCMV6- ANKZF1 was obtained from OriGene Inc (cat#: RC217418 and RC201054 TrueORF). …
Vms1 and ANKZF1 peptidyl-tRNA hydrolases release nascent chains from stalled ribosomes
Verma, Reichermeier, Burroughs et al
Nature (2018)
Verma, Reichermeier, Burroughs et al
Nature (2018)
AI Summary: OriGene’s wild-type ANKZF1 plasmid was used to express and study the role of ANKZF1 in HEK293 cells, enabling the investigation of its function in releasing nascent chains from stalled ribosomes.
Snippet: Plasmids were constructed that contained the cytomegalovirus (CMV) promoter followed by sequences encoding wild-type (Origene) or Q246L ANKZF1 (current study;confirmed by sequencing) tagged with a Flag–Myc epitope sequence at the 3′ end of the open reading frame. These constructs were transiently transfected into human embryonic kidney HEK293 cells (ATCC, tested negative for mycoplasma contamination). …
Showing 1-5 of 5 papers.
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