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ANKZF1 (NM_018089) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG201054
ANKZF1 (tGFP-tagged) - Human ankyrin repeat and zinc finger domain containing 1 (ANKZF1), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $865.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ANKZF1
Synonyms Vms1; ZNF744
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201054 representing NM_018089
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCCGGCTCCAGATGCAGCCCCGGCTCCTGCGTCGATCTCCCTGTTTGACCTCAGCGCGGATGCTC
CGGTCTTTCAGGGCCTGAGCCTGGTGAGCCACGCGCCTGGGGAGGCTCTGGCCCGGGCTCCGCGTACTTC
CTGTTCAGGCTCAGGGGAGAGAGAAAGCCCAGAAAGAAAGCTACTCCAGGGTCCTATGGATATTTCAGAG
AAGTTATTTTGTTCAACTTGTGACCAGACCTTCCAGAACCACCAAGAACAGAGGGAACATTATAAGCTTG
ACTGGCATCGGTTTAACCTAAAGCAACGTCTCAAGGACAAGCCTCTCCTGTCTGCCCTGGACTTTGAAAA
GCAGAGCTCCACAGGAGATCTTTCCAGCATCTCGGGATCAGAAGACTCAGACTCAGCCAGTGAGGAGGAC
TTGCAGACACTGGATCGGGAGAGGGCTACATTTGAGAAGTTGAGCCGACCCCCAGGCTTTTACCCTCATC
GAGTTCTTTTCCAGAATGCCCAGGGCCAGTTTCTTTATGCCTACCGCTGTGTCCTAGGCCCTCATCAGGA
TCCCCCAGAAGAGGCAGAACTGCTGCTACAGAACCTGCAAAGTAGAGGTCCCAGAGACTGCGTGGTGCTC
ATGGCTGCAGCTGGGCACTTTGCTGGTGCTATATTTCAAGGAAGAGAAGTGGTGACACACAAAACTTTTC
ACCGCTATACGGTTCGGGCCAAGCGGGGCACAGCCCAGGGGCTTCGGGATGCCCGAGGTGGGCCATCACA
CTCTGCTGGAGCCAACCTGAGGCGCTACAATGAAGCCACACTATATAAGGATGTTCGTGACCTGCTGGCA
GGGCCAAGCTGGGCTAAGGCGCTGGAGGAGGCTGGTACAATACTGTTGCGTGCTCCCCGCTCTGGCCGGT
CTTTGTTCTTTGGAGGCAAGGGAGCACCCCTGCAAAGGGGGGATCCCCGACTTTGGGATATCCCCCTCGC
CACCCGCAGACCCACCTTCCAAGAGCTACAGCGTGTGCTCCATAAGCTGACCACTTTGCATGTCTATGAA
GAAGACCCTCGGGAAGCAGTCAGACTGCACTCACCTCAGACACACTGGAAAACAGTAAGAGAGGAGAGAA
AGAAGCCTACTGAGGAAGAAATAAGAAAGATCTGCAGGGATGAAAAGGAAGCGCTGGGGCAGAATGAGGA
ATCTCCCAAACAGGGTTCAGGGTCGGAGGGAGAAGATGGCTTTCAGGTAGAGTTGGAGCTAGTGGAGTTG
ACTGTGGGGACTCTGGATCTTTGTGAGTCTGAAGTATTGCCCAAGCGGAGGAGGAGAAAAAGGAATAAGA
AGGAGAAAAGCCGAGACCAGGAGGCTGGGGCACATCGGACTCTTCTCCAGCAAACTCAAGAAGAGGAGCC
TTCCACACAGTCATCCCAGGCAGTTGCTGCCCCCTTGGGCCCTTTGCTGGATGAGGCCAAAGCCCCTGGT
CAGCCAGAGCTCTGGAATGCACTGCTTGCTGCTTGCCGAGCTGGAGATGTTGGAGTGCTAAAGCTGCAGC
TAGCTCCCAGCCCTGCAGACCCTAGAGTTCTGTCTCTGCTCAGTGCCCCCTTGGGCTCCGGTGGCTTTAC
TCTCCTGCATGCAGCAGCTGCAGCTGGAAGAGGCTCAGTGGTTCGTCTGCTGCTGGAAGCAGGTGCTGAC
CCCACTGTGCAGGACTCTCGGGCCCGGCCACCTTATACTGTTGCGGCTGACAAATCAACACGTAATGAGT
TCCGAAGGTTCATGGAGAAGAATCCAGATGCCTACGATTACAACAAGGCTCAGGTGCCAGGACCATTGAC
ACCAGAAATGGAGGCACGGCAGGCTACACGGAAAAGGGAGCAGAAGGCAGCCCGGCGGCAACGGGAGGAA
CAGCAGCAGAGGCAGCAGGAGCAGGAGGAGCGTGAACGAGAAGAGCAGCGGCGATTTGCCGCCCTCAGTG
ACCGAGAGAAGAGAGCTCTGGCTGCAGAGCGCCGACTCGCTGCCCAGTTGGGAGCCCCTACCTCTCCAAT
CCCTGACTCTGCAATCGTCAATACTCGACGCTGCTGGAGTTGTGGGGCATCCCTCCAAGGCCTGACTCCC
TTTCACTACCTCGACTTCTCTTTCTGCTCCACACGTTGCCTCCAGGATCATCGCCGTCAGGCAGGGAGGC
CCTCTTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201054 representing NM_018089
Red=Cloning site Green=Tags(s)

MSPAPDAAPAPASISLFDLSADAPVFQGLSLVSHAPGEALARAPRTSCSGSGERESPERKLLQGPMDISE
KLFCSTCDQTFQNHQEQREHYKLDWHRFNLKQRLKDKPLLSALDFEKQSSTGDLSSISGSEDSDSASEED
LQTLDRERATFEKLSRPPGFYPHRVLFQNAQGQFLYAYRCVLGPHQDPPEEAELLLQNLQSRGPRDCVVL
MAAAGHFAGAIFQGREVVTHKTFHRYTVRAKRGTAQGLRDARGGPSHSAGANLRRYNEATLYKDVRDLLA
GPSWAKALEEAGTILLRAPRSGRSLFFGGKGAPLQRGDPRLWDIPLATRRPTFQELQRVLHKLTTLHVYE
EDPREAVRLHSPQTHWKTVREERKKPTEEEIRKICRDEKEALGQNEESPKQGSGSEGEDGFQVELELVEL
TVGTLDLCESEVLPKRRRRKRNKKEKSRDQEAGAHRTLLQQTQEEEPSTQSSQAVAAPLGPLLDEAKAPG
QPELWNALLAACRAGDVGVLKLQLAPSPADPRVLSLLSAPLGSGGFTLLHAAAAAGRGSVVRLLLEAGAD
PTVQDSRARPPYTVAADKSTRNEFRRFMEKNPDAYDYNKAQVPGPLTPEMEARQATRKREQKAARRQREE
QQQRQQEQEEREREEQRRFAALSDREKRALAAERRLAAQLGAPTSPIPDSAIVNTRRCWSCGASLQGLTP
FHYLDFSFCSTRCLQDHRRQAGRPSS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018089
ORF Size 2178 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018089.3
RefSeq Size 2594 bp
RefSeq ORF 2181 bp
Locus ID 55139
UniProt ID Q9H8Y5
Cytogenetics 2q35
Summary Plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress (PubMed:28302725). Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway (By similarity).UniProtKB/Swiss-Prot Function
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"NM_018089" in other vectors (5)
SKU Description Size Price
RC201054 ANKZF1 (Myc-DDK-tagged)-Human ankyrin repeat and zinc finger domain containing 1 (ANKZF1), transcript variant 1 10 ug
$665.00
RC201054L3 Lenti ORF clone of Human ankyrin repeat and zinc finger domain containing 1 (ANKZF1), transcript variant 1, Myc-DDK-tagged 10 ug
$965.00
RC201054L4 Lenti ORF clone of Human ankyrin repeat and zinc finger domain containing 1 (ANKZF1), transcript variant 1, mGFP tagged 10 ug
$965.00
SC318211 ANKZF1 (untagged)-Human ankyrin repeat and zinc finger domain containing 1 (ANKZF1), transcript variant 1 10 ug
$732.00
SC319498 ANKZF1 (untagged)-Human ankyrin repeat and zinc finger domain containing 1 (ANKZF1), transcript variant 1 10 ug
$665.00

Plasmid Map for RG201054

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG201054
Circular map for RG201054

file-alt Citations

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