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MACROD1 (NM_014067) Human Tagged ORF Clone

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SKU
RC200992
MACROD1 (Myc-DDK-tagged)-Human MACRO domain containing 1 (MACROD1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MACROD1
Synonyms LRP16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200992 representing NM_014067
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCTACAGAGCCGACTGTCCGGCCGCCTGGCACAGCTGCGCGCGGCGGGGCAGCTGCTCGTCCCCC
CGCGCCCCCGGCCCGGACACTTGGCGGGTGCCACGAGGACCCGCAGCAGCACGTGCGGTCCCCCGGCGTT
CCTGGGCGTGTTCGGCCGCCGTGCGCGGACCTCGGCGGGAGTTGGGGCGTGGGGGGCGGCGGCGGTGGGG
CGGACAGCCGGGGTGCGCACTTGGGCCCCCCTGGCCATGGCGGCGAAGGTGGACCTGAGCACCTCCACCG
ACTGGAAGGAGGCGAAATCCTTTCTGAAGGGCCTGAGTGACAAGCAGCGGGAGGAACATTACTTCTGCAA
GGACTTTGTCAGGCTGAAGAAGATCCCGACATGGAAGGAGATGGCGAAAGGGGTGGCTGTGAAGGTGGAG
GAGCCCAGGTATAAAAAGGACAAGCAGCTCAATGAGAAAATCTCCCTGCTCCGCAGCGACATCACCAAGC
TGGAGGTGGACGCCATCGTCAACGCCGCCAACAGCTCCCTGCTCGGAGGCGGTGGCGTGGACGGCTGCAT
TCATCGGGCCGCCGGCCCCCTGCTTACCGACGAGTGCCGGACCCTGCAGAGCTGTAAGACTGGCAAGGCC
AAGATCACCGGCGGCTATCGGCTCCCGGCCAAGTACGTCATCCACACAGTGGGGCCCATCGCCTACGGGG
AGCCCAGCGCCAGCCAGGCTGCCGAGCTCCGCAGCTGCTACCTGAGCAGTCTGGACCTGCTGCTGGAGCA
CCGGCTCCGCTCGGTGGCGTTCCCCTGCATCTCCACCGGCGTGTTTGGCTACCCCTGTGAGGCGGCCGCC
GAGATCGTGCTGGCCACGCTGCGAGAGTGGCTGGAGCAGCACAAGGACAAGGTGGACCGGCTGATCATCT
GCGTGTTCCTCGAGAAGGACGAGGACATCTACCGGAGCCGGCTCCCCCACTACTTCCCCGTGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200992 representing NM_014067
Red=Cloning site Green=Tags(s)

MSLQSRLSGRLAQLRAAGQLLVPPRPRPGHLAGATRTRSSTCGPPAFLGVFGRRARTSAGVGAWGAAAVG
RTAGVRTWAPLAMAAKVDLSTSTDWKEAKSFLKGLSDKQREEHYFCKDFVRLKKIPTWKEMAKGVAVKVE
EPRYKKDKQLNEKISLLRSDITKLEVDAIVNAANSSLLGGGGVDGCIHRAAGPLLTDECRTLQSCKTGKA
KITGGYRLPAKYVIHTVGPIAYGEPSASQAAELRSCYLSSLDLLLEHRLRSVAFPCISTGVFGYPCEAAA
EIVLATLREWLEQHKDKVDRLIICVFLEKDEDIYRSRLPHYFPVA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014067
ORF Size 975 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014067.2, NP_054786.2
RefSeq Size 1225 bp
RefSeq ORF 978 bp
Locus ID 28992
UniProt ID Q9BQ69
Cytogenetics 11q13.1
MW 35.3 kDa
Summary Removes ADP-ribose from asparatate and glutamate residues in proteins bearing a single ADP-ribose moiety (PubMed:23474714, PubMed:23474712). Inactive towards proteins bearing poly-ADP-ribose (PubMed:23474714, PubMed:23474712). Deacetylates O-acetyl-ADP ribose, a signaling molecule generated by the deacetylation of acetylated lysine residues in histones and other proteins (PubMed:21257746). Plays a role in estrogen signaling (PubMed:17893710, PubMed:17914104, PubMed:19403568). Binds to androgen receptor (AR) and amplifies the transactivation function of AR in response to androgen (PubMed:19022849). May play an important role in carcinogenesis and/or progression of hormone-dependent cancers by feed-forward mechanism that activates ESR1 transactivation (PubMed:17893710, PubMed:17914104). Could be an ESR1 coactivator, providing a positive feedback regulatory loop for ESR1 signal transduction (PubMed:17914104). Could be involved in invasive growth by down-regulating CDH1 in endometrial cancer cells (PubMed:17893710). Enhances ESR1-mediated transcription activity (PubMed:17914104).UniProtKB/Swiss-Prot Function
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Documents
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"NM_014067" in other vectors (6)
SKU Description Size Price
RC200992L1 Lenti ORF clone of Human MACRO domain containing 1 (MACROD1), Myc-DDK-tagged 10 ug
$600.00
RC200992L2 Lenti ORF clone of Human MACRO domain containing 1 (MACROD1), mGFP tagged 10 ug
$600.00
RC200992L3 Lenti ORF clone of Human MACRO domain containing 1 (MACROD1), Myc-DDK-tagged 10 ug
$600.00
RC200992L4 Lenti ORF clone of Human MACRO domain containing 1 (MACROD1), mGFP tagged 10 ug
$600.00
RG200992 MACROD1 (tGFP-tagged) - Human MACRO domain containing 1 (MACROD1) 10 ug
$489.00 $500.00
SC304034 MACROD1 (untagged)-Human MACRO domain containing 1 (MACROD1) 10 ug
$300.00

Plasmid Map for RC200992

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200992
Circular map for RC200992

file-alt Citations

The ARH and Macrodomain Families of α-ADP-ribose-acceptor Hydrolases Catalyze α-NAD+ Hydrolysis
Stevens, Kato, Kasamatsu et al
ACS Chem. Biol. (2019)
AI Summary: OriGene Technologies provided human MacroD1, MacroD2, C6orf130, and PARG cDNA vectors, which were essential for expressing and studying the enzymatic activities of these proteins in the research.
Snippet: Human acroD1" style="font-weight: bold;">MacroD1, acroD2" style="font-weight: bold;">MacroD2, C6orf130, and PARG cDNA vectors were obtained from Origene Technologies. PARG catalytic domain (PARGCD)(58) was amplified from PARG cDNA vector with forward 5′-ac gcgatcgcATGAATGATTTAAATGCTAAACTACCTGGA-3′ and reverse 5′-ac acgcgtTCAGGTCCCTGTCCTTTGCCCTGAATGGTC-3′ primers
Loss of the Mono-ADP-Ribosyltransferase, TIPARP, Increases Sensitivity to Dioxin-Induced Steatohepatitis and Lethality
Ahmed, Bott, Gomez et al
J. Biol. Chem. (2015)
AI Summary: OriGene's pCMV6 Myc-DDK MacroD1 and pCMV6 Myc-DDK MacroD2 variant 1 plasmids were used as templates to amplify human MacroD1 and MacroD2 cDNAs, facilitating the study of their roles in dioxin-induced toxicity and TIPARP-mediated regulation.
Snippet: Human acroD1" style="font-weight: bold;">MacroD1 and acroD2" style="font-weight: bold;">MacroD2 cDNAs were amplified by PCR using pCMV6 Myc-DDK acroD1" style="font-weight: bold;">MacroD1 or pCMV6 Myc-DDK acroD2" style="font-weight: bold;">MacroD2 variant 1 as templates, respectively (Origene, Rockville, MD). The PCR primers for acroD1" style="font-weight: bold;">MacroD1 were forward primer 5`- ... Cite SaveSaving
Showing 1-2 of 2 papers.
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