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ALG8 (NM_024079) Human Tagged ORF Clone

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SKU
RC200914
ALG8 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $490.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ALG8
Synonyms CDG1H; PCLD3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200914 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCTCACAATTGCCACGGGTACTGGCAATTGGTTTTCGGCTTTGGCGCTCGGGGTGACTCTTC
TCAAATGCCTTCTCATCCCCACATACCATTCCACAGATTTTGAAGTACACCGAAACTGGCTTGCTATCAC
TCACAGTTTGCCAATATCACAGTGGTATTATGAGGCAACTTCAGAGTGGACGTTGGATTACCCCCCTTTC
TTTGCATGGTTTGAGTATATCCTGTCACATGTTGCCAAATATTTTGATCAAGAAATGCTGAATGTCCATA
ATTTGAATTACTCCAGCTCAAGGACCTTACTTTTCCAGAGATTTTCCGTCATCTTTATGGATGTACTCTT
TGTGTATGCTGTCCGTGAGTGCTGTAAATGCATTGATGGAAAAAAAGTGGGTAAAGAACTTACAGAAAAG
CCAAAATTTATTCTGTCGGTATTACTTCTGTGGAACTTCGGGTTATTAATTGTGGACCATATTCATTTTC
AGTACAATGGCTTTTTATTTGGATTAATGCTACTCTCCATTGCACGATTATTTCAGAAAAGGCATATGGA
AGGAGCATTTCTCTTTGCTGTTCTCCTACATTTCAAGCATATCTACCTCTATGTAGCACCAGCTTATGGT
GTATATCTGCTGCGATCCTACTGTTTCACTGCAAATAAACCAGATGGGTCTATTCGATGGAAGAGTTTCA
GCTTTGTTCGTGTTATTTCCCTGGGACTGGTTGTTTTCTTAGTTTCTGCTCTTTCATTGGGTCCTTTCCT
GGCCTTGAATCAGCTGCCTCAAGTCTTTTCCCGACTCTTTCCTTTCAAGAGGGGCCTCTGTCATGCATAT
TGGGCTCCAAACTTCTGGGCTTTGTACAATGCTTTGGACAAAGTGCTGTCTGTCATCGGTTTGAAATTGA
AATTTCTTGATCCCAACAATATTCCCAAGGCCTCAATGACAAGTGGTTTGGTTCAGCAGTTCCAACACAC
AGTCCTTCCCTCAGTGACTCCCTTGGCAACCCTCATCTGCACACTGATTGCCATATTGCCCTCTATTTTC
TGTCTTTGGTTTAAACCCCAAGGGCCCAGAGGCTTTCTCCGATGTCTAACTCTTTGTGCCTTGAGCTCCT
TTATGTTTGGGTGGCATGTTCATGAAAAAGCCATACTTCTAGCAATTCTCCCAATGAGCCTTTTGTCTGT
GGGAAAAGCAGGAGACGCTTCGATTTTTCTGATTCTGACCACAACAGGACATTATTCCCTCTTTCCTCTG
CTCTTCACTGCACCAGAACTTCCCATTAAAATCTTACTCATGTTACTATTCACCATATATAGTATTTCGT
CACTGAAGACTTTATTCAGAAAAGAAAAACCTCTTTTTAATTGGATGGAAACTTTCTACCTGCTTGGCCT
GGGGCCTCTGGAAGTCTGCTGTGAATTTGTATTCCCTTTCACCTCCTGGAAGGTGAAGTACCCCTTCATC
CCTTTGTTACTAACCTCAGTGTATTGTGCAGTAGGCGTCACATATGCTTGGTTCAAACTGTATGTTTCAG
TATTGATTGACTCTGCTATTGGCAAGACAAAGAAACAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200914 protein sequence
Red=Cloning site Green=Tags(s)

MAALTIATGTGNWFSALALGVTLLKCLLIPTYHSTDFEVHRNWLAITHSLPISQWYYEATSEWTLDYPPF
FAWFEYILSHVAKYFDQEMLNVHNLNYSSSRTLLFQRFSVIFMDVLFVYAVRECCKCIDGKKVGKELTEK
PKFILSVLLLWNFGLLIVDHIHFQYNGFLFGLMLLSIARLFQKRHMEGAFLFAVLLHFKHIYLYVAPAYG
VYLLRSYCFTANKPDGSIRWKSFSFVRVISLGLVVFLVSALSLGPFLALNQLPQVFSRLFPFKRGLCHAY
WAPNFWALYNALDKVLSVIGLKLKFLDPNNIPKASMTSGLVQQFQHTVLPSVTPLATLICTLIAILPSIF
CLWFKPQGPRGFLRCLTLCALSSFMFGWHVHEKAILLAILPMSLLSVGKAGDASIFLILTTTGHYSLFPL
LFTAPELPIKILLMLLFTIYSISSLKTLFRKEKPLFNWMETFYLLGLGPLEVCCEFVFPFTSWKVKYPFI
PLLLTSVYCAVGVTYAWFKLYVSVLIDSAIGKTKKQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024079
ORF Size 1578 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024079.2
RefSeq Size 1668 bp
RefSeq ORF 1581 bp
Locus ID 79053
UniProt ID Q9BVK2, A0A024R5K5
Cytogenetics 11q14.1
Domains Alg6_Alg8
Protein Families Transmembrane
Protein Pathways Metabolic pathways, N-Glycan biosynthesis
MW 60.1 kDa
Summary This gene encodes a member of the ALG6/ALG8 glucosyltransferase family. The encoded protein catalyzes the addition of the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation of proteins. Mutations in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ih). Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_024079" in other vectors (7)
SKU Description Size Price
RC200914L1 Lenti ORF clone of Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1, Myc-DDK-tagged 10 ug
$790.00
RC200914L2 Lenti ORF clone of Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1, mGFP tagged 10 ug
$790.00
RC200914L3 Lenti ORF clone of Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1, Myc-DDK-tagged 10 ug
$790.00
RC200914L4 Lenti ORF clone of Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1, mGFP tagged 10 ug
$790.00
RG200914 ALG8 (tGFP-tagged) - Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1 10 ug
$690.00
SC112269 ALG8 (untagged)-Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1 10 ug
$518.00
SC320615 ALG8 (untagged)-Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1 10 ug
$491.00

Plasmid Map for RC200914

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200914
Circular map for RC200914

file-alt Citations

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