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ALG8 (NM_024079) Human Untagged Clone

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SKU
SC320615
ALG8 (untagged)-Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1
  $491.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ALG8
Synonyms CDG1H; PCLD3
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_024079.4 GATCCCGGATGTGGATCAAGTTGGTGGGAAGCGTGCGGTGCCGCAGCAATGGCGGCGCTC
ACAATTGCCACGGGTACTGGCAATTGGTTTTCGGCTTTGGCGCTCGGGGTGACTCTTCTC
AAATGCCTTCTCATCCCCACATACCATTCCACAGATTTTGAAGTACACCGAAACTGGCTT
GCTATCACTCACAGTTTGCCAATATCACAGTGGTATTATGAGGCAACTTCAGAGTGGACG
TTGGATTACCCCCCTTTCTTTGCATGGTTTGAGTATATCCTGTCACATGTTGCCAAATAT
TTTGATCAAGAAATGCTGAATGTCCATAATTTGAATTACTCCAGCTCAAGGACCTTACTT
TTCCAGAGATTTTCCGTCATCTTTATGGATGTACTCTTTGTGTATGCTGTCCGTGAGTGC
TGTAAATGCATTGATGGAAAAAAAGTGGGTAAAGAACTTACAGAAAAGCCAAAATTTATT
CTGTCGGTATTACTTCTGTGGAACTTCGGGTTATTAATTGTGGACCATATTCATTTTCAG
TACAATGGCTTTTTATTTGGATTAATGCTACTCTCCATTGCACGATTATTTCAGAAAAGG
CATATGGAAGGAGCATTTCTCTTTGCTGTTCTCCTACATTTCAAGCATATCTACCTCTAT
GTAGCACCAGCTTATGGTGTATATCTGCTGCGATCCTACTGTTTCACTGCAAATAAACCA
GATGGGTCTATTCGATGGAAGAGTTTCAGCTTTGTTCGTGTTATTTCCCTGGGACTGGTT
GTTTTCTTAGTTTCTGCTCTTTCATTGGGTCCTTTCCTGGCCTTGAATCAGCTGCCTCAA
GTCTTTTCCCGACTCTTTCCTTTCAAGAGGGGCCTCTGTCATGCATATTGGGCTCCAAAC
TTCTGGGCTTTGTACAATGCTTTGGACAAAGTGCTGTCTGTCATCGGTTTGAAATTGAAA
TTTCTTGATCCCAACAATATTCCCAAGGCCTCAATGACAAGTGGTTTGGTTCAGCAGTTC
CAACACACAGTCCTTCCCTCAGTGACTCCCTTGGCAACCCTCATCTGCACACTGATTGCC
ATATTGCCCTCTATTTTCTGTCTTTGGTTTAAACCCCAAGGGCCCAGAGGCTTTCTCCGA
TGTCTAACTCTTTGTGCCTTGAGCTCCTTTATGTTTGGGTGGCATGTTCATGAAAAAGCC
ATACTTCTAGCAATTCTCCCAATGAGCCTTTTGTCTGTGGGAAAAGCAGGAGACGCTTCG
ATTTTTCTGATTCTGACCACAACAGGACATTATTCCCTCTTTCCTCTGCTCTTCACTGCA
CCAGAACTTCCCATTAAAATCTTACTCATGTTACTATTCACCATATATAGTATTTCGTCA
CTGAAGACTTTATTCAGAAAAGAAAAACCTCTTTTTAATTGGATGGAAACTTTCTACCTG
CTTGGCCTGGGGCCTCTGGAAGTCTGCTGTGAATTTGTATTCCCTTTCACCTCCTGGAAG
GTGAAGTACCCCTTCATCCCTTTGTTACTAACCTCAGTGTATTGTGCAGTAGGCGTCACA
TATGCTTGGTTCAAACTGTATGTTTCAGTATTGATTGACTCTGCTATTGGCAAGACAAAG
AAACAATGAATAAAGGAACTGCTTAGAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_024079
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024079.4, NP_076984.2
RefSeq Size 1668 bp
RefSeq ORF 1581 bp
Locus ID 79053
UniProt ID Q9BVK2
Cytogenetics 11q14.1
Domains Alg6_Alg8
Protein Families Transmembrane
Protein Pathways Metabolic pathways, N-Glycan biosynthesis
Summary This gene encodes a member of the ALG6/ALG8 glucosyltransferase family. The encoded protein catalyzes the addition of the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation of proteins. Mutations in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ih). Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the shorter transcript, but encodes the longer isoform (a).
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Documents
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"NM_024079" in other vectors (7)
SKU Description Size Price
RC200914 ALG8 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1 10 ug
$490.00
RC200914L1 Lenti ORF clone of Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1, Myc-DDK-tagged 10 ug
$790.00
RC200914L2 Lenti ORF clone of Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1, mGFP tagged 10 ug
$790.00
RC200914L3 Lenti ORF clone of Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1, Myc-DDK-tagged 10 ug
$790.00
RC200914L4 Lenti ORF clone of Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1, mGFP tagged 10 ug
$790.00
RG200914 ALG8 (tGFP-tagged) - Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1 10 ug
$690.00
SC112269 ALG8 (untagged)-Human asparagine-linked glycosylation 8, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (ALG8), transcript variant 1 10 ug
$518.00

file-alt Citations

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