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MCCC1 (NM_020166) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC200909
MCCC1 (Myc-DDK-tagged)-Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $664.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MCCC1
Synonyms MCC-B; MCCA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200909 representing NM_020166
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCCTCTGCGGTGTCGGTGCTGCTGGTGGCGGCGGAGAGGAACCGGTGGCATCGTCTCCCGA
GCCTGCTCCTGCCGCCGAGGACATGGGTGTGGAGGCAAAGAACCATGAAGTACACAACAGCCACAGGAAG
AAACATTACCAAGGTCCTCATTGCAAACAGAGGAGAAATTGCCTGCAGGGTGATGCGCACAGCCAAAAAA
CTGGGTGTACAGACTGTGGCGGTTTATAGTGAGGCTGACAGAAATTCCATGCATGTAGATATGGCAGATG
AAGCATATTCCATCGGCCCCGCTCCCTCCCAGCAGAGCTACCTATCTATGGAGAAAATCATTCAAGTGGC
CAAGACCTCTGCTGCACAGGCTATCCATCCAGGATGCGGTTTTCTCTCAGAAAACATGGAATTTGCTGAA
CTTTGTAAGCAAGAAGGAATTATTTTTATAGGCCCTCCTCCATCTGCAATTAGAGACATGGGTATAAAGA
GCACATCCAAATCCATAATGGCTGCTGCTGGAGTACCTGTTGTGGAGGGTTATCATGGTGAGGACCAATC
AGACCAGTGCCTGAAGGAACACGCCAGGAGAATTGGCTATCCTGTCATGATTAAAGCCGTCCGGGGTGGA
GGAGGAAAAGGAATGAGGATTGTTAGATCAGAACAAGAATTTCAAGAACAGTTAGAGTCAGCACGGAGAG
AAGCTAAGAAGTCTTTCAATGATGATGCTATGCTGATCGAGAAGTTTGTAGACACACCGAGGCATGTAGA
AGTCCAGGTGTTTGGTGATCACCATGGCAATGCTGTGTACTTGTTTGAAAGAGACTGTAGTGTGCAGAGG
CGACATCAGAAGATCATTGAGGAGGCCCCAGCGCCTGGTATTAAATCTGAAGTAAGAAAAAAGCTGGGAG
AAGCTGCAGTCAGAGCTGCTAAAGCTGTAAATTATGTTGGAGCAGGGACTGTGGAGTTTATTATGGACTC
AAAACATAATTTCTGTTTCATGGAGATGAATACAAGGCTGCAAGTGGAACATCCTGTTACTGAGATGATC
ACAGGAACTGACTTGGTGGAGTGGCAGCTTAGAATTGCAGCAGGAGAGAAGATTCCTTTGAGCCAGGAAG
AAATAACTCTGCAGGGCCATGCCTTCGAAGCTAGAATATATGCAGAAGATCCTAGCAATAACTTCATGCC
TGTGGCAGGCCCATTAGTGCACCTCTCTACTCCTCGAGCAGACCCTTCCACCAGGATTGAAACTGGAGTA
CGGCAAGGAGACGAAGTTTCCGTGCATTATGACCCCATGATTGCGAAGCTGGTCGTGTGGGCAGCAGATC
GCCAGGCGGCATTGACAAAACTGAGGTACAGCCTTCGTCAGTACAATATTGTTGGACTGCACACCAACAT
TGACTTCTTACTCAACCTGTCTGGCCACCCAGAGTTTGAAGCTGGGAACGTGCACACTGATTTCATCCCT
CAACACCACAAACAGTTGTTGCTCAGTCGGAAGGCTGCAGCCAAAGAGTCTTTATGCCAGGCAGCCCTGG
GTCTCATCCTCAAGGAGAAAGCCATGACCGACACTTTCACTCTTCAGGCACATGATCAATTCTCTCCATT
TTCGTCTAGCAGTGGAAGAAGACTGAATATCTCGTATACCAGAAACATGACTCTTAAAGATGGTAAAAAC
AATGTAGCCATAGCTGTAACGTATAACCATGATGGGTCTTATAGCATGCAGATTGAAGATAAAACTTTCC
AAGTCCTTGGTAATCTTTACAGCGAGGGAGACTGCACTTACCTGAAATGTTCTGTTAATGGAGTTGCTAG
TAAAGCGAAGCTGATTATCCTGGAAAACACTATTTACCTATTTTCCAAGGAAGGAAGTATTGAGATTGAC
ATTCCAGTCCCCAAATACTTATCTTCTGTGAGCTCACAAGAAACTCAGGGCGGCCCCTTAGCTCCTATGA
CTGGAACCATTGAAAAGGTGTTTGTCAAAGCTGGAGACAAAGTGAAAGCGGGAGATTCCCTCATGGTTAT
GATCGCCATGAAGATGGAGCATACCATAAAGTCTCCAAAGGATGGCACAGTAAAGAAAGTGTTCTACAGA
GAAGGTGCTCAGGCCAACAGACACACTCCTTTAGTCGAGTTTGAGGAGGAAGAATCAGACAAAAGGGAAT
CGGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200909 representing NM_020166
Red=Cloning site Green=Tags(s)

MAAASAVSVLLVAAERNRWHRLPSLLLPPRTWVWRQRTMKYTTATGRNITKVLIANRGEIACRVMRTAKK
LGVQTVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLSMEKIIQVAKTSAAQAIHPGCGFLSENMEFAE
LCKQEGIIFIGPPPSAIRDMGIKSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARRIGYPVMIKAVRGG
GGKGMRIVRSEQEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQR
RHQKIIEEAPAPGIKSEVRKKLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFCFMEMNTRLQVEHPVTEMI
TGTDLVEWQLRIAAGEKIPLSQEEITLQGHAFEARIYAEDPSNNFMPVAGPLVHLSTPRADPSTRIETGV
RQGDEVSVHYDPMIAKLVVWAADRQAALTKLRYSLRQYNIVGLHTNIDFLLNLSGHPEFEAGNVHTDFIP
QHHKQLLLSRKAAAKESLCQAALGLILKEKAMTDTFTLQAHDQFSPFSSSSGRRLNISYTRNMTLKDGKN
NVAIAVTYNHDGSYSMQIEDKTFQVLGNLYSEGDCTYLKCSVNGVASKAKLIILENTIYLFSKEGSIEID
IPVPKYLSSVSSQETQGGPLAPMTGTIEKVFVKAGDKVKAGDSLMVMIAMKMEHTIKSPKDGTVKKVFYR
EGAQANRHTPLVEFEEEESDKRESE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020166
ORF Size 2175 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020166.5
RefSeq Size 2551 bp
RefSeq ORF 2178 bp
Locus ID 56922
UniProt ID Q96RQ3
Cytogenetics 3q27.1
Domains Biotin_carb_C, biotin_lipoyl, CPSase_L_chain, CPSase_L_D2
Protein Families Druggable Genome
Protein Pathways leucine and isoleucine degradation, Metabolic pathways, Valine
MW 75.6 kDa
Summary This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. provided by RefSeq, Jul 2008
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Documents
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"NM_020166" in other vectors (4)
SKU Description Size Price
RC200909L3 Lenti ORF clone of Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$964.00
RC200909L4 Lenti ORF clone of Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$964.00
RG200909 MCCC1 (tGFP-tagged) - Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein 10 ug
$864.00
SC113201 MCCC1 (untagged)-Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein 10 ug
$665.00

Plasmid Map for RC200909

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200909
Circular map for RC200909

file-alt Citations

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