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MCCC1 (NM_020166) Human Untagged Clone

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SKU
SC113201
MCCC1 (untagged)-Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein
  $665.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MCCC1
Synonyms MCC-B; MCCA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC113201 sequence for NM_020166 edited (data generated by NextGen Sequencing)
ATGGCGGCGGCCTCTGCGGTGTCGGTGCTGCTGGTGGCGGCGGAGAGGAACCGGTGGCAT
CGTCTCCCGAGCCTGCTCCTGCCGCCGAGGACATGGGTGTGGAGGCAAAGAACCATGAAG
TACACAACAGCCACAGGAAGAAACATTACCAAGGTCCTCATTGCAAACAGAGGAGAAATT
GCCTGCAGGGTGATGCGCACAGCCAAAAAACTGGGTGTACAGACTGTGGCGGTTTATAGT
GAGGCTGACAGAAATTCCATGCATGTAGATATGGCAGATGAAGCATATTCCATCGGCCCC
GCTCCCTCCCAGCAGAGCTACCTATCTATGGAGAAAATCATTCAAGTGGCCAAGACCTCT
GCTGCACAGGCTATCCATCCAGGATGCGGTTTTCTTTCAGAAAACATGGAATTTGCTGAA
CTTTGTAAGCAAGAAGGAATTATTTTTATAGGCCCTCCTCCATCTGCAATTAGAGACATG
GGTATAAAGAGCACATCCAAATCCATAATGGCTGCTGCTGGAGTACCTGTTGTGGAGGGT
TATCATGGTGAGGACCAATCAGACCAGTGCCTGAAGGAACACGCCAGGAGAATTGGCTAT
CCTGTCATGATTAAAGCCGTCCGGGGTGGAGGAGGAAAAGGAATGAGGATTGTTAGATCA
GAACAAGAATTTCAAGAACAGTTAGAGTCAGCACGGAGAGAAGCTAAGAAGTCTTTCAAT
GATGATGCTATGCTGATCGAGAAGTTTGTAGACACACCGAGGCATGTAGAAGTCCAGGTG
TTTGGTGATCACCATGGCAATGCTGTGTACTTGTTTGAAAGAGACTGTAGTGTGCAGAGG
CGACATCAGAAGATCATTGAGGAGGCCCCAGCGCCTGGTATTAAATCTGAAGTAAGAAAA
AAGCTGGGAGAAGCTGCAGTCAGAGCTGCTAAAGCTGTAAATTATGTTGGAGCAGGGACT
GTGGAGTTTATTATGGACTCAAAACATAATTTCTGTTTCATGGAGATGAATACAAGGCTG
CAAGTGGAACATCCTGTTACTGAGATGATCACAGGAACTGACTTGGTGGAGTGGCAGCTT
AGAATTGCAGCAGGAGAGAAGATTCCTTTGAGCCAGGAAGAAATAACTCTGCAGGGCCAT
GCCTTCGAAGCTAGAATATATGCAGAAGATCCTAGCAATAACTTCATGCCTGTGGCAGGC
CCATTAGTGCACCTCTCTACTCCTCGAGCAGACCCTTCCACCAGGATTGAAACTGGAGTA
CGGCAAGGAGACGAAGTTTCCGTGCATTATGACCCCATGATTGCGAAGCTGGTCGTGTGG
GCAGCAGATCGCCAGGCGGCATTGACAAAACTGAGGTACAGCCTTCGTCAGTACAATATT
GTTGGACTGCCCACCAACATTGACTTCTTACTCAACCTGTCTGGCCACCCAGAGTTTGAA
GCTGGGAACGTGCACACTGATTTCATCCCTCAACACCACAAACAGTTGTTGCTCAGTCGG
AAGGCTGCAGCCAAAGAGTCTTTATGCCAGGCAGCCCTGGGTCTCATCCTCAAGGAGAAA
GCCATGACCGACACTTTCACTCTTCAGGCACATGATCAATTCTCTCCATTTTCGTCTAGC
AGTGGAAGAAGACTGAATATCTCGTATACCAGAAACATGACTCTTAAAGATGGTAAAAAC
AATGTAGCCATAGCTGTAACGTATAACCATGATGGGTCTTATAGCATGCAGATTGAAGAT
AAAACTTTCCAAGTCCTTGGTAATCTTTACAGCGAGGGAGACTGCACTTACCTGAAATGT
TCTGTTAATGGAGTTGCTAGTAAAGCGAAGCTGATTATCCTGGAAAACACTATTTACCTA
TTTTCCAAGGAAGGAAGTATTGAGATTGACATTCCAGTCCCCAAATACTTATCTTCTGTG
AGCTCACAAGAAACTCAGGGCGGCCCCTTAGCTCCTATGACTGGAACCATTGAAAAGGTG
TTTGTCAAAGCTGGAGACAAAGTGAAAGCGGGAGATTCCCTCATGGTTATGATCGCCATG
AAGATGGAGCATACCATAAAGTCTCCAAAGGATGGCACAGTAAAGAAAGTGTTCTACAGA
GAAGGTGCTCAGGCCAACAGACACACTCCTTTAGTCGAGTTTGAGGAGGAAGAATCAGAC
AAAAGGGAATCGGAATAA

Clone variation with respect to NM_020166.3
396 c=>t;1391 a=>c
5' Read Nucleotide Sequence
>OriGene 5' read for NM_020166 unedited
GGTTGTTCAGNNATATTTGTNAATACGCACTTCACTATAGNNGGCGGCCGCGCAATTCGG
CACGAGGGCCCGCGGGGGTCACGAGGCTTCGTAGTGGAGGAACGGGTTTGGCGTGTGGGA
CGCAGCTGCCTCTGTACTGGGGAGTCACGGAGTGGCCGGGCTCCAGGGACATGGCGGCGG
CCTCTGCGGTGTCGGTGCTGCTGGTGGCGGCGGAGAGGAACCGGTGGCATCGTCTCCCGA
GCCTGCTCCTGCCGCCGAGGACATGGGTGTGGAGGCAAAGAACCATGAAGTACACAACAG
CCACAGGAAGAAACATTACCAAGGTCCTCATTGCAAACAGAGGAGAAATTGCCTGCAGGG
TGATGCGCACAGCCAAAAAACTGGGTGTACAGACTGTGGCGGTTTATAGTGAGGCTGACA
GAAATTCCATGCATGTAGATATGGCAGATGAAGCATATTCCATCGGCCCCGCTCCCTCCC
AGCAGAGCTACCTATCTATGGAGAAAATCATTCAAGTGGCCAAGACCTCTGCTGCACAGG
CTATCCATCCAGGATGCGGTTTTCTTTCAGAAAACATGGAATTTGCTGAACTTTGTAAGC
AAGAAGGAATTATTTTTATAGGCCCTCCTCCATCTGCAATTAGAGACATGGGTATAAAGA
GCACATCCAAATCCATAATGGCTGCTGCTGGAGTACCTGTTGTGGCACGGTTATCATGGT
GAGGACCAATCAGACCAGTGCCTGGAGGAACACGCCAGNAGAATTGGCTATCCTGTCATG
ATTAAAGCCGCCCGGGCTGGGAGGAAGGAAAATGATTGGACGCATTGGTTCCACCCCGAA
ACAAGAATTTCTCAGAACCCGTTCACAGCCACGCACGGAAAGGAGCTTCAAAAACCTCTT
TCAACGGATGACGCCTATGCCTGATTCGAAAAC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_020166 unedited
GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCAAAAAAAAAAATTCACTTTTTTTATTTT
AAAATTTTTGGAAAAAATTAGGGCCCCAAAGGCTTGATTTTCCAATTTAAAGGGGGTTCA
CCTTAACCTTCCAATTTTTTAAAAAAACTGTTTTTTATAAAACCCCCAAAAAAGCGGGGG
GCCTTTCTTTTTTTTGGGGGAAAAAAAAAACCTTTTTTAACGGGCCTTTTCTTTGGGGGA
GTTAATCCCAATCCCCTTTGGGCGGATTTTTCCTCCCAAAACTCAATTAAAGGAGGGTGT
TTTTTGGCCTAACCCCCTTTTTTGAAAAACATTTTTTTTACTGGGCCTTCTTTTGAAAAC
TTTAGGGGAGCCCCCATTTTCGGGGGGATCTTAACCTTGGGGGAATCTCCCGTTTTCATT
TTGTCCCCAGTTTTAAAAAACACCTTTTAAAGGGTTCCAACCATAGAACCTAAGGGGCCC
CCCTGATTTTTTTGGGACCCCACAAAAAATAATTTTTGGGGGACGGGAATGCCAATCCCA
ATACTTCCTTCCTTGAAAAAAGGAAAATAGGGTTTCCCAGAAAAAATCACTTTCTTTTTA
CTACCAACTCCTTTAAAAAAACATTTAGGGTAAGGGCAGCCCCCCTCGCTGAAAAAATAC
CAAGGCCTTGGAAAGTTTTATCTCAATTTGCTTGCTATAAAACCCCCAGGGTTATCCCTA
CACCTATGGCTACTTGTTTTCCACATTTTTAAAAAACATGTTTCGGAGTAACAAGATATT
AATTTTTTTTCACTGTGTAACAAAATGGGAAAATTTTCTTTTGTCCCGAAAAAGGAAAGT
TCCGCCTTGGTT
Restriction Sites NotI-NotI |
ACCN NM_020166
Insert Size 2620 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020166.2, NP_064551.2
RefSeq Size 2528 bp
RefSeq ORF 2178 bp
Locus ID 56922
UniProt ID Q96RQ3
Cytogenetics 3q27.1
Domains Biotin_carb_C, biotin_lipoyl, CPSase_L_chain, CPSase_L_D2
Protein Families Druggable Genome
Protein Pathways leucine and isoleucine degradation, Metabolic pathways, Valine
Summary This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) encodes the longer isoform (1).
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Documents
Resources
"NM_020166" in other vectors (4)
SKU Description Size Price
RC200909 MCCC1 (Myc-DDK-tagged)-Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein 10 ug
$589.00 $664.00
RC200909L3 Lenti ORF clone of Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$964.00
RC200909L4 Lenti ORF clone of Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$964.00
RG200909 MCCC1 (tGFP-tagged) - Human methylcrotonoyl-CoA carboxylase 1 (alpha) (MCCC1), nuclear gene encoding mitochondrial protein 10 ug
$864.00

file-alt Citations

Leucine regulates autophagy via acetylation of the mTORC1 component raptor
Son, Park, Stamatakou et al
Nat Commun (2020) 11 (1), 3148 DOI: 10.1038/s41467-020-16886-2
Cited by 125
AI Summary: OriGene’s pCMV6-XL5-MCCC1 plasmid (#SC113201) was used in this study to investigate the molecular mechanisms by which leucine regulates autophagy via mTORC1 component raptor acetylation.
Snippet: fection. The following DNA or siRNA/shRNA constructs were also used in this work: empty pEGFP (Clontech), pEGFP-α-synuclein A53T and pcDNA3.1-myc-6XHis (Invitrogen);pCMV6-XL5-MCCC1 (#SC113201) from Origene;pcDNA3.1-EP300-6XHis (#23252), pRK5-HA-YFP-raptor (#73385), pRK5-HA-raptor (#8513), pcDNA4-BECN1-Flag (#24388) from Addgene. Huntington’s disease gene exon 1 fragment with 74 poly-Q repeats
FigureFigureFigure
Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A
Son, Park, Lee et al
Cell Metab. (2018)
AI Summary: In this paper, the OriGene product pCMV6-XL5-MCCC1 (#SC113201) was used as a recombinant DNA construct to study the role of leucine metabolism in regulating mTORC1 activity.
Snippet: c ctacagggccaccgtg-3’ This paper N/A For siRNA sequences see Table S1 N/A N/A ------------------------- Recombinant DNA ------------------------- pEFGP Clontech pCMV6-XL5-MCCC1 Origene #SC113201 pcDNA3.1-EP300 Addgene #23252 pcDNA3.1-EP300(HAT-) Addgene #23254 peYFP-C1-mTOR Addgene #73384 pRK5-HA-YFP-Raptor Addgene #73385 pRK5-HA-YFP-PRAS40 Addg;... c ctacagggccaccgtg-3’ This paper N/A For siRNA sequences see Table S1 N/A N/A ------------------------- Recombinant DNA ------------------------- pEFGP Clontech pCMV6-XL5-MCCC1 Origene #SC113201 pcDNA3.1-EP300 Addgene #23252 pcDNA3.1-EP300(HAT-) Addgene #23254 peYFP-C1-mTOR Addgene #73384 pRK5-HA-YFP-Raptor Addgene #73385 pRK5-HA-YFP-PRAS40 Addg;;For siRNA sequences see Table S1, N/A, N/A. … pEFGP, Clontech, pCMV6-XL5-MCCC1, Origene, #SC113201. …
Selectivity in post-translational biotin addition to five human carboxylases
Ingaramo, Beckett
J. Biol. Chem. (2012) 287 (3), 1813-22 DOI: 10.1074/jbc.M111.275982
AI Summary: OriGene contributed the cDNA for subunit A of methylcrotonyl-CoA carboxylase, which was used to amplify the MCC BCCP fragment for biotinylation studies in this paper.
Snippet: The SUMO fusion plasmid used for expression of the 67-amino acid-long C-terminal region of PCC carboxylase was described previously (8). This minimal ... The cDNA for subunit A of methyl crotonyl CoA carboxylase (Origene, Inc.) was used to amplify the MCC BCCP fragment.
Showing 1-3 of 3 papers.
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