HSCB (NM_172002) Human Tagged ORF Clone

SKU RC200843

HSCB (Myc-DDK-tagged)-Human HscB iron-sulfur cluster co-chaperone homolog (E. coli) (HSCB)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: HSCB
• Synonyms: DNAJC20; HSC20; JAC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200843 representing NM_172002; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTGGCGGGGGAGAGCCGGGGCTTTGCTCCGGGTGTGGGGGTTTTGGCCGACAGGGGTTCCCAGAAGGA; GACCGCTAAGCTGCGATGCTGCGTCGCAGGCGGGAAGCAATTATCCCCGCTGTTGGAACTGCGGCGGCCC; ATGGGGCCCCGGGCGGGAGGACAGGTTCTTCTGCCCACAGTGCCGAGCGCTGCAGGCACCTGACCCCACT; CGAGACTACTTCAGCCTTATGGACTGCAACCGTTCCTTCAGAGTTGATACAGCGAAGCTCCAGCACAGGT; ACCAGCAACTGCAGCGTCTTGTCCACCCAGATTTCTTCAGCCAGAGGTCTCAGACTGAAAAGGACTTCTC; AGAGAAGCATTCGACCCTGGTGAATGATGCCTATAAGACCCTCCTGGCCCCCCTGAGCAGAGGACTGTAC; CTTCTAAAGCTCCATGGAATAGAGATTCCTGAAAGGACAGATTATGAAATGGACAGGCAATTCCTCATAG; AAATAATGGAAATCAATGAAAAACTCGCAGAAGCTGAAAGTGAAGCTGCCATGAAAGAGATTGAATCCAT; TGTCAAAGCTAAACAGAAAGAATTTACTGACAATGTGAGCAGTGCTTTTGAACAAGATGACTTTGAAGAA; GCCAAGGAAATTTTGACAAAGATGAGATACTTTTCAAATATAGAAGAAAAGATCAAGTTAAAGAAGATTC; CCCTT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200843 representing NM_172002; Red=Cloning site Green=Tags(s); ; MWRGRAGALLRVWGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCGGPWGPGREDRFFCPQCRALQAPDPT; RDYFSLMDCNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQTEKDFSEKHSTLVNDAYKTLLAPLSRGLY; LLKLHGIEIPERTDYEMDRQFLIEIMEINEKLAEAESEAAMKEIESIVKAKQKEFTDNVSSAFEQDDFEE; AKEILTKMRYFSNIEEKIKLKKIPL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172002
• ORF Size: 705 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_172002.5
• RefSeq Size: 1106 bp
• RefSeq ORF: 708 bp
• Locus ID: 150274
• UniProt ID: Q8IWL3
• Cytogenetics: 22q12.1
• MW: 27.2 kDa
• Summary: This gene encodes a DnaJ-type co-chaperone and member of the heat shock cognate B (HscB) family of proteins. The encoded protein plays a role in the synthesis of iron-sulfur clusters, protein cofactors that are involved in the redox reactions of mitochondrial electron transport and other processes. Cells in which this gene is knocked down exhibit reduced activity of iron-sulfur cluster-dependent enzymes including succinate dehydrogenase and aconitase. The encoded protein may stimulate the ATPase activity of the mitochondrial stress-70 protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]