USP10 (NM_005153) Human Tagged ORF Clone
$731.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | USP10 |
| Synonyms | UBPO |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005153 |
| ORF Size | 2394 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005153.1, NP_005144.1 |
| RefSeq Size | 3399 bp |
| RefSeq ORF | 2397 bp |
| Locus ID | 9100 |
| UniProt ID | Q14694 |
| Cytogenetics | 16q24.1 |
| Domains | UCH |
| Protein Families | Druggable Genome, Protease |
| MW | 87.2 kDa |
| Summary | Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene. provided by RefSeq, Jan 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005153" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200835L1 | Lenti ORF clone of Human ubiquitin specific peptidase 10 (USP10), Myc-DDK-tagged | 10 ug |
$1,031.00
|
|
| RC200835L2 | Lenti ORF clone of Human ubiquitin specific peptidase 10 (USP10), mGFP tagged | 10 ug |
$1,031.00
|
|
| RC200835L3 | Lenti ORF clone of Human ubiquitin specific peptidase 10 (USP10), Myc-DDK-tagged | 10 ug |
$1,031.00
|
|
| RC200835L4 | Lenti ORF clone of Human ubiquitin specific peptidase 10 (USP10), mGFP tagged | 10 ug |
$1,031.00
|
|
| RG200835 | USP10 (tGFP-tagged) - Human ubiquitin specific peptidase 10 (USP10) | 10 ug |
$789.00
$931.00
|
|
| SC111097 | USP10 (untagged)-Human ubiquitin specific peptidase 10 (USP10) | 10 ug |
$732.00
|
|
Citations
The deubiquitinase USP10 regulates KLF4 stability and suppresses lung tumorigenesis
Wang, Xia, Li et al
Cell Death Differ. (2019)
Wang, Xia, Li et al
Cell Death Differ. (2019)
AI Summary: In this study, OriGene products including the USP10 cDNA clone (RC208447) and KLF4 cDNA clone (RC204222) were used to overexpress and investigate the roles of USP10 and KLF4 in regulating protein stability and lung tumorigenesis.
Snippet: …Here, by screening ubiquitin-specific proteases that may interact with KLF4, we found ubiquitin-specific peptidase 10 (USP10) as a deubiquitinating enzyme for KLF4. Forced expression of USP10 remarkably increases KLF4 protein level by blocking the latter degradation, whereas the depletion of USP10 promotes KLF4 degradation and thus enhances tumorigenesis. Loss of USP10 in mice downregulates KLF4 expression and accelerates KrasG12D-driven lung adenocarcinoma initiation and progression. … Clinically, reduction of USP10 expression, concomitant with decreased KLF4 and TIMP3 abundance in carcinoma tissue, predicts poor prognosis of lung cancer patient. Taken together, our results demonstrate that USP10 is a critical regulator of KLF4, pinpointing USP10-KLF4-TIMP3 axis as a promising therapeutic target in lung cancer
TFAM is a novel mediator of immunogenic cancer cell death
Yang, Li, Zhu et al
Oncoimmunology (2018) 7 (6), e1431086 DOI: 10.1080/2162402X.2018.1431086
Yang, Li, Zhu et al
Oncoimmunology (2018) 7 (6), e1431086 DOI: 10.1080/2162402X.2018.1431086
AI Summary: OriGene products contributed to this study by providing the TFAM antibody (Cat# TA303519) and AGER antibody (Cat# TA326608), which were used to neutralize TFAM and AGER and thereby demonstrate their essential roles in spautin-1-induced immunogenic cancer cell death.
TFAM is a novel mediator of immunogenic cancer cell death
Yang, Li, Zhu et al
OncoImmunology (2018)
Yang, Li, Zhu et al
OncoImmunology (2018)
Showing 1-3 of 3 papers.
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