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USP10 (NM_005153) Human Untagged Clone

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SKU
SC111097
USP10 (untagged)-Human ubiquitin specific peptidase 10 (USP10)
  $732.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol USP10
Synonyms UBPO
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC111097 sequence for NM_005153 edited (data generated by NextGen Sequencing)
ATGGCCCTCCACAGCCCGCAGTATATTTTTGGAGATTTTAGCCCTGATGAATTCAATCAA
TTCTTTGTGACTCCTCGATCTTCAGTTGAGCTTCCTCCATACAGTGGAACAGTTCTGTGT
GGCACACAGGCTGTGGATAAACTACCTGATGGACAAGAATATCAGAGAATTGAGTTTGGT
GTCGATGAAGTCATTGAACCCAGTGACACTTTGCCGAGAACCCCCAGCTACAGTATTTCA
AGCACACTGAACCCTCAGGCCCCTGAATTTATTCTCGGTTGTACAGCTTCCAAAATAACC
CCTGATGGTATCACTAAAGAAGCAAGCTATGGCTCCATCGACTGCCAGTACCCAGGCTCT
GCCCTCGCTTTGGATGGAAGTTCTAATGTGGAGGCGGAAGTTTTGGAAAATGATGGTGTC
TCAGGTGGTCTTGGACAAAGGGAGCGTAAAAAGAAGAAAAAGCGGCCACCTGGATATTAC
AGCTATTTGAAAGATGGTGGCGATGATAGTATCTCCACAGAAGCCCTGGTCAATGGCCAT
GCCAATTCAGCAGTCCCGAACAGTGTCAGTGCAGAGGATGCAGAATTTATGGGTGACATG
CCCCCGTCAGTTACGCCCAGGACTTGTAACAGCCCCCAGAACTCCACAGACTCTGTCAGT
GACATTGTGCCTGATAGTCCTTTCCCCGGAGCACTCGGCAGTGACACCAGGACTGCAGGG
CAGCCAGAGGGGGGCCCCGGGGCTGATTTTGGTCAGTCCTGCTTCCCTGCAGAGGCTGGC
AGAGACACCCTGTCAAGGACAGCTGGGGCTCAGCCCTGCGTTGGTACCGATACTACTGAA
AACCTTGGAGTTGCTAATGGACAAATACTTGAATCCTCGGGTGAGGGCACAGCTACCAAC
GGGGTGGAGTTGCACACCACGGAAAGCATAGACTTGGACCCAACCAAACCCGAGAGTGCA
TCACCTCCTGCTGACGGCACGGGCTCTGCATCAGGCACCCTTCCTGTCAGCCAGCCCAAG
TCCTGGGCCAGCCTCTTTCATGATTCTAAGCCCTCTTCCTCCTCGCCGGTGGCCTATGTG
GAAACTAAGTATTCCCCTCCCGCCATATCTCCCCTGGTTTCTGAAAAGCAGGTTGAAGTC
AAAGAAGGGCTTGTTCCGGTGTCAGAGGATCCTGTAGCCATAAAGATTGCAGAGTTGCTG
GAGAATGTAACCCTAATCCATAAACCAGTGTCGTTGCAACCCCGTGGGCTGATCAATAAA
GGGAACTGGTGCTACATTAATGCTACACTGCAGGCATTGGTTGCTTGCCCGCCGATGTAC
CACCTGATGAAGTTCATTCCTCTGTATTCCAAAGTGCAAAGGCCTTGTACGTCAACACCC
ATGATAGACAGCTTTGTTCGGCTAATGAATGAGTTCACTAATATGCCAGTACCTCCAAAA
CCCCGACAAGCTCTTGGAGATAAAATCGTGAGGGATATTCGCCCTGGAGCTGCCTTTGAG
CCCACATATATTTACAGACTCCTGACAGTTAACAAGTCAAGCCTGTCTGAAAAGGGTCGA
CAGGAAGATGCTGAGGAATACTTAGGCTTCATTCTAAATGGACTTCATGAGGAAATGTTG
AACCTAAAGAAGCTTCTCTCACCAAGTAATGAAAAACTTACGATTTCCAACGGCCCCAAA
AACCACTCGGTCAATGAAGAAGAGCAGGAAGAACAAGGTGAAGGAAGCGAGGATGAATGG
GAACAAGTGGGCCCCCGGAACAAGACTTCCGTCACCCGCCAGGCGGATTTTGTTCAGACT
CCAATCACCGGCATTTTTGGTGGACACATCAGGTCTGTGGTTTACCAGCAGAGTTCAAAA
GAATCTGCCACTTTGCAGCCATTTTTCACGTTGCAGTTGGATATCCAGTCAGACAAGATA
CGCACAGTCCAGGATGCACTGGAGAGCTTGGTGGCAAGAGAATCTGTCCAAGGTTATACC
ACAAAAACCAAACAAGAGGTTGAGATAAGTCGAAGAGTGACTCTGGAAAAACTCCCTCCT
GTCCTCGTGCTGCACCTGAAACGATTCGTTTATGAGAAGACTGGTGGGTGCCAGAAGCTT
ATCAAAAATATTGAATATCCTGTGGACTTGGAAATTAGTAAAGAACTGCTTTCTCCAGGG
GTTAAAAATAAGAATTTTAAATGCCACCGAACCTATCGGCTGTTTGCAGTGGTCTACCAT
CACGGCAACAGTGCGACGGGCGGCCATTACACTACAGACGTCTTCCAGATCGGTCTGAAT
GGCTGGCTGCGCATCGATGACCAGACAGTCAAGGTGATCAACCAGTACCAGGTGGTGAAA
CCAACTGCTGAACGCACAGCCTACCTCCTGTATTACCGCCGAGTGGACCTGCTGTAA

Clone variation with respect to NM_005153.2
675 c=>t;1161 t=>g;1563 a=>g;2202 c=>g
5' Read Nucleotide Sequence
>OriGene 5' read for NM_005153 unedited
NNNAAGGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGAAG
AGGCGGCGGCGGGGGAAGCAGCGTGAGCAGCCGGAGGATCGCGGAGTCCCAATGAAACGG
GCAGCCATGGCCCTCCACAGCCCGCAGTATATTTTTGGAGATTTTAGCCCTGATGAATTC
AATCAATTCTTTGTGACTCCTCGATCTTCAGTTGAGCTTCCTCCATACAGTGGAACAGTT
CTGTGTGGCACACAGGCTGTGGATAAACTACCTGATGGACAAGAATATCAGAGAATTGAG
TTTGGTGTCGATGAAGTCATTGAACCCAGTGACACTTTGCCGAGAACCCCCAGCTACAGT
ATTTCAAGCACACTGAACCCTCAGGCCCCTGAATTTATTCTCGGTTGTACAGCTTCCAAA
ATAACCCCTGATGGTATCACTAAAGAAGCAAGCTATGGCTCCATCGACTGCCAGTACCCA
GGCTCTGCCCTCGCTTTGGATGGAAGTTCTAATGTGGAGGCGGAAGTTTTGGAAAATGAT
GGTGTCTCAGGTGGTCTTGGACAAGGGAGCGTAAAAAGAGAAAAAGCGGCCACCTGGATA
TTACAGCTATTTGAAAGATGGTGGCGATGATAGTATCTCCACAGAAGCCCTGGTCAATGG
CCATGCCAATTCAGCAGTCCCGAACAGTGTCAGTGCAGAGGATGCAGAATTTATGGGTGA
CATGCCCCCGTCAGTTACGCCCAGNACTTNGTACAGCCCCCAGAACTNCACAGACTCTGT
CAGTGACATTGTGCCTGATAGTCCTTTCCCCGGACACTCGGCAGTGACACCAGGACTGCA
GGNCAGCCAGAGGGGGGGCCCCGGNGCTGATNTTGGTCAGTCCTGCTTCCCTGCAGAGGC
TGGCAGAGACACCCTGT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005153 unedited
NNGGGTACGACTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTAAAACCAACTTTGGGATTTTTATTGAGGGGCGAC
AACTTTATACTCCTAGATATCACTAAACTGGGTACAATTAGGGACCCCGCATTAGAGAAA
AGCAGACCGCAGAATTAAACAGAGCAGACTGGAGGAGAGATCTTCATTATTTGCCCATTT
TTCATTATGTGTACCCAGAAGCCTGAATGCCATTTGAAATCTTTTAATGGCAATAAAGTT
ACAATCACCCATCTATGTAGACTAACATTTTAACTCCAAATATTTGATCTGCAATGGGTA
CCTAAGCAGTTTCTCTCAGTACAATTATTAAAATTTTTCCTGGTAGGAACCAGCAACCTA
TTTTTTAAGGTTATTTTTCTTTTGAAGGAAAAACTAATTCTTCTTTGATAACCGGCCCAT
TTTTATCATTTATCAAAAACTAAAGGGTAGGGAAAAAAAGTGTGATGGATTAAAAAATTT
CTTTTTTAAGGGAAGATAAAATTCATTTTCACAAATTTACCAATGTTGTTGCTGGTGCCA
GATTTATTCCACTAAGCCATGAGACTGGGGATCAAATCCACTTTCTTATCTCAAGAATCA
GCCTTATTTCAGAAAATGGGGTTTTGGGGATTTTCTAAATCAAGCAACAGTCTGTTTCAA
CCCAATGATTTTTGATTGGAAGTTAAAAGTCACAAAAACTGGGTTTGTGCACCAACCCCC
AGGGGATCTCTTTTTATTCTAGCCCATTTTTGACAAGGGGGAAAGAGTTCTCTCTCAAGA
ACCCCTTAAGAAAAGCCGGAAATGAATGGGAAGGGGGGGTCCCACAAACCGGGACCGGGG
AC
Restriction Sites NotI-NotI |
ACCN NM_005153
Insert Size 3500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005153.1, NP_005144.1
RefSeq Size 3009 bp
RefSeq ORF 2397 bp
Locus ID 9100
UniProt ID Q14694
Cytogenetics 16q24.1
Domains UCH
Protein Families Druggable Genome, Protease
Summary Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene. provided by RefSeq, Jan 2013
Transcript Variant: This variant (2) lacks an alternate exon in the 5' end compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.
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Documents
Resources
"NM_005153" in other vectors (6)
SKU Description Size Price
RC200835 USP10 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 10 (USP10) 10 ug
$731.00
RC200835L1 Lenti ORF clone of Human ubiquitin specific peptidase 10 (USP10), Myc-DDK-tagged 10 ug
$1,031.00
RC200835L2 Lenti ORF clone of Human ubiquitin specific peptidase 10 (USP10), mGFP tagged 10 ug
$1,031.00
RC200835L3 Lenti ORF clone of Human ubiquitin specific peptidase 10 (USP10), Myc-DDK-tagged 10 ug
$1,031.00
RC200835L4 Lenti ORF clone of Human ubiquitin specific peptidase 10 (USP10), mGFP tagged 10 ug
$1,031.00
RG200835 USP10 (tGFP-tagged) - Human ubiquitin specific peptidase 10 (USP10) 10 ug
$789.00 $931.00

file-alt Citations

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