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Integrin beta 5 (ITGB5) (NM_002213) Human Tagged ORF Clone

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SKU
RC200731
ITGB5 (Myc-DDK-tagged)-Human integrin, beta 5 (ITGB5)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,097.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Integrin beta 5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200731 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCGGGCCCCGGCGCCGCTGTACGCCTGCCTCCTGGGGCTCTGCGCGCTCCTGCCCCGGCTCGCAG
GTCTCAACATATGCACTAGTGGAAGTGCCACCTCATGTGAAGAATGTCTGCTAATCCACCCAAAATGTGC
CTGGTGCTCCAAAGAGGACTTCGGAAGCCCACGGTCCATCACCTCTCGGTGTGATCTGAGGGCAAACCTT
GTCAAAAATGGCTGTGGAGGTGAGATAGAGAGCCCAGCCAGCAGCTTCCATGTCCTGAGGAGCCTGCCCC
TCAGCAGCAAGGGTTCGGGCTCTGCAGGCTGGGACGTCATTCAGATGACACCACAGGAGATTGCCGTGAA
CCTCCGGCCCGGTGACAAGACCACCTTCCAGCTACAGGTTCGCCAGGTGGAGGACTATCCTGTGGACCTG
TACTACCTGATGGACCTCTCCCTGTCCATGAAGGATGACTTGGACAATATCCGGAGCCTGGGCACCAAAC
TCGCGGAGGAGATGAGGAAGCTCACCAGCAACTTCCGGTTGGGATTTGGGTCTTTTGTTGATAAGGACAT
CTCTCCTTTCTCCTACACGGCACCGAGGTACCAGACCAATCCGTGCATTGGTTACAAGTTGTTTCCAAAT
TGCGTCCCCTCCTTTGGGTTCCGCCATCTGCTGCCTCTCACAGACAGAGTGGACAGCTTCAATGAGGAAG
TTCGGAAACAGAGGGTGTCCCGGAACCGAGATGCCCCTGAGGGGGGCTTTGATGCAGTACTCCAGGCAGC
CGTCTGCAAGGAGAAGATTGGCTGGCGAAAGGATGCACTGCATTTGCTGGTGTTCACAACAGATGATGTG
CCCCACATCGCATTGGATGGAAAATTGGGAGGCCTGGTGCAGCCACACGATGGCCAGTGCCACCTGAACG
AGGCCAACGAGTACACTGCATCCAACCAGATGGACTATCCATCCCTTGCCTTGCTTGGAGAGAAATTGGC
AGAGAACAACATCAACCTCATCTTTGCAGTGACAAAAAACCATTATATGCTGTACAAGAATTTTACAGCC
CTGATACCTGGAACAACGGTGGAGATTTTAGATGGAGACTCCAAAAATATTATTCAACTGATTATTAATG
CATACAATAGTATCCGGTCTAAAGTGGAGTTGTCAGTCTGGGATCAGCCTGAGGATCTTAATCTCTTCTT
TACTGCTACCTGCCAAGATGGGGTATCCTATCCTGGTCAGAGGAAGTGTGAGGGTCTGAAGATTGGGGAC
ACGGCATCTTTTGAAGTATCATTGGAGGCCCGAAGCTGTCCCAGCAGACACACGGAGCATGTGTTTGCCC
TGCGGCCGGTGGGATTCCGGGACAGCCTGGAGGTGGGGGTCACCTACAACTGCACGTGCGGCTGCAGCGT
GGGGCTGGAACCCAACAGTGCCAGGTGCAACGGGAGCGGGACCTATGTCTGCGGCCTGTGTGAGTGCAGC
CCCGGCTACCTGGGCACCAGGTGCGAGTGCCAGGATGGGGAGAACCAGAGCGTGTACCAGAACCTGTGCC
GGGAGGCAGAGGGCAAGCCACTGTGCAGCGGGCGTGGGGACTGCAGCTGCAACCAGTGCTCCTGCTTCGA
GAGCGAGTTCGGCAAGATCTATGGGCCTTTCTGTGAGTGCGACAACTTCTCCTGTGCCAGGAACAAGGGA
GTCCTCTGCTCAGGCCATGGCGAGTGTCACTGCGGGGAATGCAAGTGCCATGCAGGTTACATCGGGGACA
ACTGTAACTGCTCGACAGACATCAGCACATGCCGGGGCAGAGATGGCCAGATCTGCAGCGAGCGTGGGCA
CTGTCTCTGTGGGCAGTGCCAATGCACGGAGCCGGGGGCCTTTGGGGAGATGTGTGAGAAGTGCCCCACC
TGCCCGGATGCATGCAGCACCAAGAGAGATTGCGTCGAGTGCCTGCTGCTCCACTCTGGGAAACCTGACA
ACCAGACCTGCCACAGCCTATGCAGGGATGAGGTGATCACATGGGTGGACACCATCGTGAAAGATGACCA
GGAGGCTGTGCTATGTTTCTACAAAACCGCCAAGGACTGCGTCATGATGTTCACCTATGTGGAGCTCCCC
AGTGGGAAGTCCAACCTGACCGTCCTCAGGGAGCCAGAGTGTGGAAACACCCCCAACGCCATGACCATCC
TCCTGGCTGTGGTCGGTAGCATCCTCCTTGTTGGGCTTGCACTCCTGGCTATCTGGAAGCTGCTTGTCAC
CATCCACGACCGGAGGGAGTTTGCAAAGTTTCAGAGCGAGCGATCCAGGGCCCGCTATGAAATGGCTTCA
AATCCATTATACAGAAAGCCTATCTCCACGCACACTGTGGACTTCACCTTCAACAAGTTCAACAAATCCT
ACAATGGCACTGTGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200731 protein sequence
Red=Cloning site Green=Tags(s)

MPRAPAPLYACLLGLCALLPRLAGLNICTSGSATSCEECLLIHPKCAWCSKEDFGSPRSITSRCDLRANL
VKNGCGGEIESPASSFHVLRSLPLSSKGSGSAGWDVIQMTPQEIAVNLRPGDKTTFQLQVRQVEDYPVDL
YYLMDLSLSMKDDLDNIRSLGTKLAEEMRKLTSNFRLGFGSFVDKDISPFSYTAPRYQTNPCIGYKLFPN
CVPSFGFRHLLPLTDRVDSFNEEVRKQRVSRNRDAPEGGFDAVLQAAVCKEKIGWRKDALHLLVFTTDDV
PHIALDGKLGGLVQPHDGQCHLNEANEYTASNQMDYPSLALLGEKLAENNINLIFAVTKNHYMLYKNFTA
LIPGTTVEILDGDSKNIIQLIINAYNSIRSKVELSVWDQPEDLNLFFTATCQDGVSYPGQRKCEGLKIGD
TASFEVSLEARSCPSRHTEHVFALRPVGFRDSLEVGVTYNCTCGCSVGLEPNSARCNGSGTYVCGLCECS
PGYLGTRCECQDGENQSVYQNLCREAEGKPLCSGRGDCSCNQCSCFESEFGKIYGPFCECDNFSCARNKG
VLCSGHGECHCGECKCHAGYIGDNCNCSTDISTCRGRDGQICSERGHCLCGQCQCTEPGAFGEMCEKCPT
CPDACSTKRDCVECLLLHSGKPDNQTCHSLCRDEVITWVDTIVKDDQEAVLCFYKTAKDCVMMFTYVELP
SGKSNLTVLREPECGNTPNAMTILLAVVGSILLVGLALLAIWKLLVTIHDRREFAKFQSERSRARYEMAS
NPLYRKPISTHTVDFTFNKFNKSYNGTVD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002213
ORF Size 2397 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002213.3, NP_002204.2
RefSeq Size 3392 bp
RefSeq ORF 2400 bp
Locus ID 3693
UniProt ID P18084, L7RT22
Cytogenetics 3q21.2
Domains EGF, INB, PSI, VWA
Protein Families Druggable Genome, Transmembrane
Protein Pathways Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
MW 88.1 kDa
Summary This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. Integrins are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin. provided by RefSeq, Aug 2017
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Documents
Resources
"NM_002213" in other vectors (6)
SKU Description Size Price
RC200731L1 Lenti ORF clone of Human integrin, beta 5 (ITGB5), Myc-DDK-tagged 10 ug
$1,397.00
RC200731L2 Lenti ORF clone of Human integrin, beta 5 (ITGB5), mGFP tagged 10 ug
$1,397.00
RC200731L3 Lenti ORF clone of Human integrin, beta 5 (ITGB5), Myc-DDK-tagged 10 ug
$1,397.00
RC200731L4 Lenti ORF clone of Human integrin, beta 5 (ITGB5), mGFP tagged 10 ug
$1,397.00
RG200731 ITGB5 (tGFP-tagged) - Human integrin, beta 5 (ITGB5) 10 ug
$1,297.00
SC118751 ITGB5 (untagged)-Human integrin, beta 5 (ITGB5) 10 ug
$1,098.00

Plasmid Map for RC200731

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200731
Circular map for RC200731

file-alt Citations

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