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LSP1 (NM_002339) Human Tagged ORF Clone

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SKU
RC200712
LSP1 (Myc-DDK-tagged)-Human lymphocyte-specific protein 1 (LSP1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "LSP1 or NM_002339"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LSP1
Synonyms pp52; WP34
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200712 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGCTTCGAGTGACCCGGGTGCTGAGGAGCGGGAAGAGTTGCTGGGGCCCACTGCTCAGTGGA
GCGTGGAGGACGAGGAGGAGGCCGTCCACGAGCAATGCCAGCATGAGAGAGACAGGCAGCTTCAGGCCCA
GGACGAGGAGGGAGGCGGCCATGTCCCCGAGCGGCCGAAGCAGGAGATGCTCCTCAGCCTGAAGCCCTCG
GAGGCCCCTGAACTGGATGAGGACGAGGGCTTTGGCGACTGGTCCCAGAGGCCAGAGCAGCGGCAGCAGC
ACGAGGGGGCGCAGGGCACCTTGGACAGCGGAGAGCCCCCCCAGTGCAGGAGTCCTGAGGGGGAGCAAGA
GGACAGGCCCGGCCTGCATGCCTACGAAAAGGAGGACAGTGATGAAGTCCACCTGGAGGAGTTGAGTCTG
AGCAAGGAGGGGCCAGGCCCAGAGGACACTGTCCAGGACAACCTGGGGGCCGCAGGGGCTGAGGAGGAAC
AGGAGGAGCACCAGAAATGTCAGCAGCCCAGGACACCCAGCCCCTTGGTCTTGGAGGGGACCATCGAACA
GAGCTCGCCTCCCCTGAGCCCTACCACCAAACTCATCGACAGGACCGAGTCCCTAAACCGCTCCATAGAG
AAGAGTAACAGTGTGAAGAAATCCCAGCCAGACTTGCCCATCTCCAAGATTGATCAGTGGCTGGAACAAT
ACACCCAGGCCATCGAGACCGCTGGCCGGACCCCCAAGCTAGCCCGCCAGGCCTCCATAGAGCTGCCCAG
CATGGCTGTGGCCAGTACCAAGAGTCGGTGGGAGACGGGTGAGGTACAGGCTCAGTCTGCGGCCAAGACT
CCGTCCTGCAAGGATATTGTGGCTGGAGACATGAGCAAGAAAAGCCTCTGGGAGCAGAAGGGAGGCTCCA
AGACCTCATCAACAATTAAGAGCACCCCATCTGGGAAGAGGTATAAGTTTGTGGCCACCGGGCATGGGAA
GTATGAGAAGGTGCTTGTGGAAGGGGGCCCGGCTCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200712 protein sequence
Red=Cloning site Green=Tags(s)

MAEASSDPGAEEREELLGPTAQWSVEDEEEAVHEQCQHERDRQLQAQDEEGGGHVPERPKQEMLLSLKPS
EAPELDEDEGFGDWSQRPEQRQQHEGAQGTLDSGEPPQCRSPEGEQEDRPGLHAYEKEDSDEVHLEELSL
SKEGPGPEDTVQDNLGAAGAEEEQEEHQKCQQPRTPSPLVLEGTIEQSSPPLSPTTKLIDRTESLNRSIE
KSNSVKKSQPDLPISKIDQWLEQYTQAIETAGRTPKLARQASIELPSMAVASTKSRWETGEVQAQSAAKT
PSCKDIVAGDMSKKSLWEQKGGSKTSSTIKSTPSGKRYKFVATGHGKYEKVLVEGGPAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002339
ORF Size 1017 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002339.3
RefSeq Size 1756 bp
RefSeq ORF 1020 bp
Locus ID 4046
UniProt ID P33241
Cytogenetics 11p15.5
MW 37.2 kDa
Summary This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jul 2008
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"NM_002339" in other vectors (6)
SKU Description Size Price
RC200712L1 Lenti ORF clone of Human lymphocyte-specific protein 1 (LSP1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC200712L2 Lenti ORF clone of Human lymphocyte-specific protein 1 (LSP1), transcript variant 1, mGFP tagged 10 ug
$757.00
RC200712L3 Lenti ORF clone of Human lymphocyte-specific protein 1 (LSP1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC200712L4 Lenti ORF clone of Human lymphocyte-specific protein 1 (LSP1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG200712 LSP1 (tGFP-tagged) - Human lymphocyte-specific protein 1 (LSP1), transcript variant 1 10 ug
$489.00 $657.00
SC118682 LSP1 (untagged)-Human lymphocyte-specific protein 1 (LSP1), transcript variant 1 10 ug
$457.00

Plasmid Map for RC200712

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200712
Circular map for RC200712

file-alt Citations

Fucose-specific DC-SIGN signalling directs T helper cell type-2 responses via IKKε- and CYLD-dependent Bcl3 activation
Gringhuis, Kaptein, Wevers et al
Nat Commun (2014) 5, 3898 DOI: 10.1038/ncomms4898
Cited by 157
AI Summary: OriGene contributed to this study by providing the pCMV6-Entry Myc-DDK-tagged LSP1 expression plasmid, which was used to investigate the role of LSP1 in DC-SIGN signaling and T helper cell type-2 immune responses.
Snippet: LSP1 expression plasmid pCMV6-Entry Myc-DDK-tagged ORF LSP1 was obtained from Origene. LSP1 mutants S204A and S252A were generated using QuikChange II XL site-directed mutagenesis kit (Stratagene) and mutations were verified by sequencing. LSP1 coding sequences were subcloned into lentiviral construct pRRL-cPPT-CMV-X2-PRE-SIN-IRES-eGFP53. … LSP1 mutant expressing cells were sorted based on GFP expression using a BD Influx cell sorter (BD)
Showing 1-1 of 1 papers.
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