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LSP1 (NM_002339) Human Untagged Clone

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SKU
SC118682
LSP1 (untagged)-Human lymphocyte-specific protein 1 (LSP1), transcript variant 1
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol LSP1
Synonyms pp52; WP34
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_002339, the custom clone sequence may differ by one or more nucleotides


ATGGCGGAGGCTTCGAGTGACCCGGGTGCCGAGGAGCGGGAAGAGTTGCTGGGGCCCACTGCTCAGTGGA
GCGTGGAGGACGAGGAGGAGGCCGTCCACGAGCAATGCCAGCATGAGAGAGACAGGCAGCTTCAGGCCCA
GGACGAGGAGGGAGGCGGCCATGTCCCCGAGCGGCCGAAGCAGGAGATGCTCCTCAGCCTGAAGCCCTCG
GAGGCCCCTGAACTGGATGAGGACGAGGGCTTTGGCGACTGGTCCCAGAGGCCAGAGCAGCGGCAGCAGC
ACGAGGGGGCGCAGGGCGCCTTGGACAGCGGAGAGCCCCCCCAGTGCAGGAGTCCTGAGGGGGAGCAAGA
GGACAGGCCCGGCCTGCATGCCTACGAAAAGGAGGACAGTGATGAAGTCCACCTGGAGGAGTTGAGTCTG
AGCAAGGAGGGGCCAGGCCCAGAGGACACTGTCCAGGACAACCTGGGGGCCGCAGGGGCTGAGGAGGAAC
AGGAGGAGCACCAGAAATGTCAGCAGCCCAGGACACCCAGCCCCTTGGTCTTGGAGGGGACCATCGAACA
GAGCTCGCCTCCCCTGAGCCCTACCACCAAACTCATCGACAGGACCGAGTCCCTAAACCGCTCCATAGAG
AAGAGTAACAGTGTGAAGAAATCCCAGCCAGACTTGCCCATCTCCAAGATTGATCAGTGGCTGGAACAAT
ACACCCAGGCCATCGAGACCGCTGGCCGGACCCCCAAGCTAGCCCGCCAGGCCTCCATAGAGCTGCCCAG
CATGGCTGTGGCCAGTACCAAGAGTCGGTGGGAGACGGGTGAGGTACAGGCTCAGTCTGCGGCCAAGACT
CCGTCCTGCAAGGATATTGTGGCTGGAGACATGAGCAAGAAAAGCCTCTGGGAGCAGAAGGGAGGCTCCA
AGACCTCATCAACAATTAAGAGCACCCCATCTGGGAAGAGGTATAAGTTTGTGGCCACCGGGCATGGGAA
GTATGAGAAGGTGCTTGTGGAAGGGGGCCCGGCTCCCTAG

5' Read Nucleotide Sequence
>OriGene 5' read for NM_002339 unedited
ACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGGCTGCTACCCATGACGGG
TTGGGGAGGTATTGGTGCGAGCTGAGGTGGTGCTTGCGGAGGCCCACTGCTCAGTGGAGC
GTGGAGGACGAGGAGGAGGCCGTCCACGAGCAATGCCAGCATGAGAGAGACAGGCAGCTT
CAGGCCCAGGACGAGGAGGGAGGCGGCCATGTCCCCGAGCGGCCGAAGCAGGAGATGCTC
CTCAGCCTGAAGCCCTCGGAGGCCCCTGAACTGGATGAGGACGAGGGCTTTGGCGACTGG
TCCCAGAGGCCAGAGCAGCGGCAGCAGCACGAGGGGGCGCAGGGCGCCTTGGACAGCGGA
GAGCCCCCCCAGTGCAGGAGTCCTGAGGGGGAGCAAGAGGACAGGCCCGGCCTGCATGCC
TACGAAAAGGAGGACAGTGATGAAGTCCACCTGGAGGAGTTGAGTCTGAGCAAGGAGGGG
CCAGGCCCAGAGGACACTGTCCAGGACAACCTGNGGGCCGCAGGGGCTGAGGAGGAACAG
GAGGAGCACCAGAAATGTCAGCAGCCCAGGACACCCAGCCNCTTGGTCTTGGAGGGGACC
ATCGAACAGAGCTCGCCTCCCCTGAGCCCTACCACCAAACTCATCGACAGGACCGAGTCC
CTAAACCGCTCCATAGAGAAGAGTAACAGTGTGAAGAAATCCCAGCCAGACTTGCCCATC
TNNCAGATTGATCAGTGGCTGGAACAATACANCCCAGCCATCGAGACCGCTGGCCGGACC
CCCAGCTAGCCCGCCAGGCCTCATAGAGCTGCCAGCATGGCTGTGGCCGTACCANNAGTC
GTGGAGACGGNTGAGGTACAGCTANTCTGNGCAAAACTCGTCTGCAGGTATTGTGGCTGA
GACATGACAGAAAGCTTGGACAGAGGNAGCTCAGACTCTACATANACCCTCTGGAAAGGG
TTAGT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_002339 unedited
GAACCGCGNGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGAGAAACACAGGGA
GCTTTATTATACAAAATGGCGGGGTGGGGGGCGGCAAGCAGCGGGGGACGCAGCATCAAG
CATCCTGCATGGCCGTTATCAGCCCTTGACCTGCAGTTTCCCCTTGGATCTGGGGGGGTG
ACCCACCCTCTCTGCACAGGCTGTGCCTCAACCTCCTAACTTCCTAAAAGGCACTTGGCC
TCTCCAGGGGTAAGTCCCTTTGGCCAATGATCAGGAGTTTCTTTCCTCCCCCAAGTAACA
AGAAGCGGAGCTGGGTGGGGAGGGCGGGGGCGGTGGTCATAGAGTGGAAGGGCAGAGAGG
CTAAAGGGTCCAGGAGCAAAAGGAGGTGGCAGCGGATGGCATCAAAGAGGCGAGGGTAGG
TCATGCTGGCAACAGGAAGCAACTTCTTATCCAGGGCCGGGGGGCGGGTGTCTGGCTGGA
ATCTCCCCTGGGTACATGGAGGGTGCCAGCCGGCTGGACCTGCAAACCCAGGAAGCCAGA
TGGGACCCCTAGGGAACCGGGCCCCCTTCCACAGCACCTTCTCATACCTTCCATGTCCCG
GGGGCCACAAACTTATAACCTCTTCCCAAACGGGGCGCCCTTACTGGTCGATGAGGTTTT
TGGAACCCCCCTTTTGCTCACAAAAGCCTTTTCTTGCTCATGTCTCCAACCCCCATATCC
CTTGCAAGACCGGAGTTTTTGTCCGTAAACATGTACCCTGTACCTTACACCGTTCTCACA
CCTAACTCTTTGTATCTGGTCCACCACCATGCCCTGGCCATCTTTCTATGAAAGGCTCCG
GTCGGGTTAATCTCCGACGTGTCACGCGTACAGCCCCATCACACGGCCACGTTCCGCTAT
TCTCCCACCCCACCCGATATAATCTCTGCCACAGTCTCCATCCCCGAGCTTTCTATTTCT
TACTACACTGCATCTCTTTTTTACGATACCCCC
Restriction Sites NotI-NotI |
ACCN NM_002339
Insert Size 1610 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002339.2, NP_002330.1
RefSeq Size 1756 bp
RefSeq ORF 1020 bp
Locus ID 4046
UniProt ID P33241
Cytogenetics 11p15.5
Summary This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) contains an alternate exon and lacks two exons in the 5' coding region compared to variant 5. The resulting protein (isoform 1) has a distinct N-terminus and is shorter than isoform 3.
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Documents
Resources
"NM_002339" in other vectors (6)
SKU Description Size Price
RC200712 LSP1 (Myc-DDK-tagged)-Human lymphocyte-specific protein 1 (LSP1), transcript variant 1 10 ug
$457.00
RC200712L1 Lenti ORF clone of Human lymphocyte-specific protein 1 (LSP1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC200712L2 Lenti ORF clone of Human lymphocyte-specific protein 1 (LSP1), transcript variant 1, mGFP tagged 10 ug
$757.00
RC200712L3 Lenti ORF clone of Human lymphocyte-specific protein 1 (LSP1), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC200712L4 Lenti ORF clone of Human lymphocyte-specific protein 1 (LSP1), transcript variant 1, mGFP tagged 10 ug
$757.00
RG200712 LSP1 (tGFP-tagged) - Human lymphocyte-specific protein 1 (LSP1), transcript variant 1 10 ug
$489.00 $657.00

file-alt Citations

Lymphocyte-specific protein 1 inhibits the growth of hepatocellular carcinoma by suppressing ERK1/2 phosphorylation
Zhang, Wang, Liu et al
FEBS Open Bio (2016) 6 (12), 1227-1237 DOI: 10.1002/2211-5463.12139
Cited by 16
AI Summary: OriGene’s human LSP1 cDNA clone (RC202541) was used in this study to overexpress LSP1 in hepatocellular carcinoma cell lines, enabling the authors to investigate its role in suppressing tumor growth and ERK1/2 phosphorylation.
FigureFigureFigure
LSP1 inhibits the growth of hepatocellular carcinoma by suppressing ERK1/2 phosphorylation
Zhang, Wang, Liu et al
FEBS Open Bio (2016)
AI Summary: OriGene contributed to the study by providing the pcDNA/LSP1 plasmid (sc118682), which was used for cell transfection experiments to investigate the role of LSP1 in hepatocellular carcinoma growth.
Snippet: software (BD Biosciences). Cell transfection The pcDNA/LSP1 and Control werepurchased from Origene Biotechnology(sc118682,Origene biotechnology, Beijing, China).Theday before transfection, 5 10 6 Hep3B or HepG2 cells were seeded in 100 mm dishes. ... Cite SaveSaving
Showing 1-2 of 2 papers.
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