CDK2 (NM_001798) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CDK2 |
| Synonyms | CDKN2; p33(CDK2) |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001798 |
| ORF Size | 894 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001798.5 |
| RefSeq Size | 2301 bp |
| RefSeq ORF | 897 bp |
| Locus ID | 1017 |
| UniProt ID | P24941 |
| Cytogenetics | 12q13.2 |
| Domains | pkinase, S_TKc, TyrKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Cell cycle, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Small cell lung cancer |
| MW | 33.9 kDa |
| Summary | This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001798" in other vectors (8)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200494L1 | Lenti ORF clone of Human cyclin-dependent kinase 2 (CDK2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC200494L2 | Lenti ORF clone of Human cyclin-dependent kinase 2 (CDK2), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RC200494L3 | Lenti ORF clone of Human cyclin-dependent kinase 2 (CDK2), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC200494L4 | Lenti ORF clone of Human cyclin-dependent kinase 2 (CDK2), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RG200494 | CDK2 (tGFP-tagged) - Human cyclin-dependent kinase 2 (CDK2), transcript variant 1 | 10 ug |
$650.00
|
|
| SC109060 | CDK2 (untagged)-Human cyclin-dependent kinase 2 (CDK2), transcript variant 1 | 10 ug |
$300.00
|
|
| SC323390 | CDK2 (untagged)-Kinase deficient mutant (K33M) of Human cyclin-dependent kinase 2 (CDK2), transcript variant 1 | 10 ug |
$300.00
|
|
| SC323701 | CDK2 (untagged)-Kinase deficient mutant (K33M) of Human cyclin-dependent kinase 2 (CDK2), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
PAK4 phosphorylates cyclin-dependent kinase 2 to promote the G1/S transition during adipogenesis
Yu, Park, Jo et al
Exp Mol Med (2025) 57 (9), 2121-2132 DOI: 10.1038/s12276-025-01525-x
Yu, Park, Jo et al
Exp Mol Med (2025) 57 (9), 2121-2132 DOI: 10.1038/s12276-025-01525-x
Cited by 4
AI Summary: OriGene’s human CDK2 plasmid vector (#RC200494) was used to generate mutant constructs for investigating the phosphorylation of cyclin-dependent kinase 2 by PAK4 during adipogenesis in this study.
Snippet: y autoradiography. For Coomassie blue staining, the gel was stained for 1 h in protein staining buffer (#ab119211, Abcam). Mutagenesis Mutant constructs of the human CDK2 plasmid vector (#RC200494, Origene) were generated using a site-directed mutagenesis kit (#EZ004S, Enzynomics). Specifically, the CDK2S106A, CDK2S106D and CDK2T160A variants were created by introducing point mutations that subs
SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis
Guo, Lei, Chang et al
Signal Transduct Target Ther (2022) 7 (1), 400 DOI: 10.1038/s41392-022-01239-w
Guo, Lei, Chang et al
Signal Transduct Target Ther (2022) 7 (1), 400 DOI: 10.1038/s41392-022-01239-w
Cited by 26
AI Summary: OriGene's Myc-CDK2 plasmid (Cat# RC200494) was used to express CDK2 in cells, enabling the study of its interaction with SARS-CoV-2 nsp12 and its role in viral RNA synthesis in this research.
Snippet: as described in detail previously.26 Nsp12 mutant constructs, containing the combined T20A and T20E mutation were obtained using Fast Mutagenesis System (TransGen Biotech). Myc-CDK2 was obtained from Origene (Cat# RC200494) and pLVX-CCNA2-HA-IRES-Puro was purchased from ZOMANBIO Co., Ltd (Cat# ZK7080). Human Flag-CDK1 and EGFP-CyclinB were cloned into the pSG5 vector and EGFP-N1 vector in our lab
CDK2 kinase activity is a regulator of male germ cell fate
Singh, Patel, Palmer et al
Development (2019) 146 (21) DOI: 10.1242/dev.180273
Singh, Patel, Palmer et al
Development (2019) 146 (21) DOI: 10.1242/dev.180273
Cited by 23
AI Summary: In the paper "CDK2 kinase activity is a regulator of male germ cell fate," OriGene products, specifically the anti-DDX4 antibody (Cat# TA800872) and anti-STRA8 antibody (Cat# TA322486), were used for immunostaining to identify and analyze germ cell populations during the study of spermatogonial differentiation defects.
Showing 1-4 of 4 papers.
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