ERH (NM_004450) Human Tagged ORF Clone
$150.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | ERH |
| Synonyms | DROER |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004450 |
| ORF Size | 312 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004450.3 |
| RefSeq Size | 801 bp |
| RefSeq ORF | 315 bp |
| Locus ID | 2079 |
| UniProt ID | P84090, A0A024R6D4 |
| Cytogenetics | 14q24.1 |
| Domains | ER |
| MW | 12.3 kDa |
| Summary | May have a role in the cell cycle.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004450" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200367L1 | Lenti ORF clone of Human enhancer of rudimentary homolog (Drosophila) (ERH), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC200367L2 | Lenti ORF clone of Human enhancer of rudimentary homolog (Drosophila) (ERH), mGFP tagged | 10 ug |
$450.00
|
|
| RC200367L3 | Lenti ORF clone of Human enhancer of rudimentary homolog (Drosophila) (ERH), Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC200367L4 | Lenti ORF clone of Human enhancer of rudimentary homolog (Drosophila) (ERH), mGFP tagged | 10 ug |
$450.00
|
|
| RG200367 | ERH (tGFP-tagged) - Human enhancer of rudimentary homolog (Drosophila) (ERH) | 10 ug |
$489.00
|
|
| SC110885 | ERH (untagged)-Human enhancer of rudimentary homolog (Drosophila) (ERH) | 10 ug |
$150.00
|
|
Citations
Apoptotic Effects of Anthocyanins from Vitis coignetiae Pulliat Are Enhanced by Augmented Enhancer of the Rudimentary Homolog (ERH) in Human Gastric Carcinoma MKN28 Cells
Park, Lee, Go et al
Int J Mol Sci (2021) 22 (6) DOI: 10.3390/ijms22063030
Park, Lee, Go et al
Int J Mol Sci (2021) 22 (6) DOI: 10.3390/ijms22063030
Cited by 8
AI Summary: In this study, OriGene’s ERH expression plasmid (RC210438) and ERH-specific siRNA (SR303009) were used to modulate ERH levels in MKN28 gastric cancer cells, enabling the authors to demonstrate that ERH augmentation enhances the apoptotic effects of anthocyanins from Vitis coignetiae Pulliat.
Apoptotic Effects of Anthocyanins from Vitis coignetiae Pulliat Are Enhanced by Augmented Enhancer of the Rudimentary Homolog (ERH) in Human Gastric Carcinoma MKN28 Cells
Park, Lee, Go et al
IJMS (2021) 22 (6), 3030 DOI: 10.3390/ijms22063030
Park, Lee, Go et al
IJMS (2021) 22 (6), 3030 DOI: 10.3390/ijms22063030
Cited by 8
AI Summary: OriGene’s Human ERH cDNA (RC200367) was used to overexpress ERH in gastric cancer cells, enabling the study of how ERH augmentation enhances the apoptotic effects of anthocyanins from Vitis coignetiae in this research.
Snippet: Human ERH cDNA was purchased by OriGene (RC200367). Cells were transfected with ERH-expressing plasmids using Lipofectamine 3000 (Invitrogen;Thermo Fisher Scientific, Inc) as per the manufacturer’s instructions
ERH overexpression is associated with decreased cell migration and invasion and a good prognosis in gastric cancer
Park, Park, Park et al
Transl Cancer Res (2020) 9 (9), 5281-5291 DOI: 10.21037/tcr-20-1498
Park, Park, Park et al
Transl Cancer Res (2020) 9 (9), 5281-5291 DOI: 10.21037/tcr-20-1498
Cited by 12
AI Summary: OriGene products contributed to this study by providing ERH cDNA clones and ERH-specific siRNAs, which were used to generate ERH-overexpressing and ERH-knockdown gastric cancer cell lines, enabling the functional analysis of ERH in cell migration and invasion.
Showing 1-3 of 3 papers.
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