ERH (NM_004450) Human Tagged ORF Clone

  • TrueORF®
SKU
RG200367
ERH (tGFP-tagged) - Human enhancer of rudimentary homolog (Drosophila) (ERH)
  $489.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ERH
Synonyms DROER
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG200367 representing NM_004450
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCACACCATTTTGCTGGTACAGCCTACCAAGAGGCCAGAAGGCAGAACTTATGCTGACTACGAAT
CTGTGAATGAATGCATGGAAGGTGTTTGTAAAATGTATGAAGAACATCTGAAAAGAATGAATCCCAACAG
TCCCTCTATCACATATGACATCAGTCAGTTGTTTGATTTCATCGATGATCTGGCAGACCTCAGCTGCCTG
GTTTACCGAGCTGATACCCAGACATACCAGCCTTATAACAAAGACTGGATTAAAGAGAAGATCTACGTGC
TCCTTCGTCGGCAGGCCCAACAGGCTGGGAAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG200367 representing NM_004450
Red=Cloning site Green=Tags(s)

MSHTILLVQPTKRPEGRTYADYESVNECMEGVCKMYEEHLKRMNPNSPSITYDISQLFDFIDDLADLSCL
VYRADTQTYQPYNKDWIKEKIYVLLRRQAQQAGK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004450
ORF Size 312 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004450.3
RefSeq Size 815 bp
RefSeq ORF 315 bp
Locus ID 2079
UniProt ID P84090
Cytogenetics 14q24.1
Domains ER
Summary May have a role in the cell cycle.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_004450" in other vectors (6)
SKU Description Size Price
RC200367 ERH (Myc-DDK-tagged)-Human enhancer of rudimentary homolog (Drosophila) (ERH) 10 ug
$150.00
RC200367L1 Lenti ORF clone of Human enhancer of rudimentary homolog (Drosophila) (ERH), Myc-DDK-tagged 10 ug
$450.00
RC200367L2 Lenti ORF clone of Human enhancer of rudimentary homolog (Drosophila) (ERH), mGFP tagged 10 ug
$450.00
RC200367L3 Lenti ORF clone of Human enhancer of rudimentary homolog (Drosophila) (ERH), Myc-DDK-tagged 10 ug
$450.00
RC200367L4 Lenti ORF clone of Human enhancer of rudimentary homolog (Drosophila) (ERH), mGFP tagged 10 ug
$450.00
SC110885 ERH (untagged)-Human enhancer of rudimentary homolog (Drosophila) (ERH) 10 ug
$150.00

Plasmid Map for RG200367

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Cloning scheme for RG200367

Circular map for RG200367

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