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XRCC3 (NM_005432) Human Tagged ORF Clone

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SKU
RC200326
XRCC3 (Myc-DDK-tagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol XRCC3
Synonyms CMM6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200326 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATTTGGATCTACTGGACCTGAATCCCAGAATTATTGCTGCAATTAAGAAAGCCAAACTGAAATCGG
TAAAGGAGGTTTTACACTTTTCTGGACCAGACTTGAAGAGACTGACCAACCTCTCCAGCCCCGAGGTCTG
GCACTTGCTGAGAACGGCCTCCTTACACTTGCGGGGAAGCAGCATCCTTACAGCACTGCAGCTGCACCAG
CAGAAGGAGCGGTTCCCCACGCAGCACCAGCGCCTGAGCCTGGGCTGCCCGGTGCTGGACGCGCTGCTCC
GCGGTGGCCTGCCCCTGGACGGCATCACTGAGCTGGCCGGACGCAGCTCGGCAGGGAAGACCCAGCTGGC
GCTGCAGCTCTGCCTGGCTGTGCAGTTCCCGCGGCAGCACGGAGGCCTGGAGGCTGGAGCCGTCTACATC
TGCACGGAAGACGCCTTCCCGCACAAGCGCCTGCAGCAGCTCATGGCCCAGCAGCCGCGGCTGCGCACTG
ACGTTCCAGGAGAGCTGCTTCAGAAGCTCCGATTTGGCAGCCAGATCTTCATCGAGCACGTGGCCGATGT
GGACACCTTGTTGGAGTGTGTGAATAAGAAGGTCCCCGTACTGCTGTCTCGGGGCATGGCTCGCCTGGTG
GTCATCGACTCGGTGGCAGCCCCATTCCGCTGTGAATTTGACAGCCAGGCCTCCGCCCCCAGGGCCAGGC
ATCTGCAGTCCCTGGGGGCCACGCTGCGTGAGCTGAGCAGTGCCTTCCAGAGCCCTGTGCTGTGCATCAA
CCAGGTGACAGAGGCCATGGAGGAGCAGGGCGCAGCACACGGGCCGCTGGGGTTCTGGGACGAACGTGTT
TCCCCAGCCCTTGGCATAACCTGGGCTAACCAGCTCCTGGTGAGACTGCTGGCTGACCGGCTCCGCGAGG
AAGAGGCTGCCCTCGGCTGCCCAGCCCGGACCCTGCGGGTGCTCTCTGCCCCCCACCTGCCCCCCTCCTC
CTGTTCCTACACGATCAGTGCCGAAGGGGTGCGAGGGACACCTGGGACCCAGTCCCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200326 protein sequence
Red=Cloning site Green=Tags(s)

MDLDLLDLNPRIIAAIKKAKLKSVKEVLHFSGPDLKRLTNLSSPEVWHLLRTASLHLRGSSILTALQLHQ
QKERFPTQHQRLSLGCPVLDALLRGGLPLDGITELAGRSSAGKTQLALQLCLAVQFPRQHGGLEAGAVYI
CTEDAFPHKRLQQLMAQQPRLRTDVPGELLQKLRFGSQIFIEHVADVDTLLECVNKKVPVLLSRGMARLV
VIDSVAAPFRCEFDSQASAPRARHLQSLGATLRELSSAFQSPVLCINQVTEAMEEQGAAHGPLGFWDERV
SPALGITWANQLLVRLLADRLREEEAALGCPARTLRVLSAPHLPPSSCSYTISAEGVRGTPGTQSH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005432
ORF Size 1038 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005432.4
RefSeq Size 2620 bp
RefSeq ORF 1041 bp
Locus ID 7517
UniProt ID O43542
Cytogenetics 14q32.33
Protein Families Druggable Genome
Protein Pathways Homologous recombination
MW 37.8 kDa
Summary This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Alternatively spliced transcript variants encoding the same protein have been identified. provided by RefSeq, Jul 2008
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"NM_005432" in other vectors (6)
SKU Description Size Price
RC200326L1 Lenti ORF clone of Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC200326L2 Lenti ORF clone of Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2, mGFP tagged 10 ug
$757.00
RC200326L3 Lenti ORF clone of Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC200326L4 Lenti ORF clone of Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2, mGFP tagged 10 ug
$757.00
RG200326 XRCC3 (tGFP-tagged) - Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2 10 ug
$489.00 $657.00
SC116733 XRCC3 (untagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 3 (XRCC3), transcript variant 2 10 ug
$457.00

Plasmid Map for RC200326

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200326
Circular map for RC200326

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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