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ASF1A (NM_014034) Human Tagged ORF Clone

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SKU
RC200324
ASF1A (Myc-DDK-tagged)-Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ASF1A
Synonyms CGI-98; CIA; HSPC146
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200324 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAAGGTTCAGGTGAACAATGTAGTGGTGCTGGATAACCCTTCTCCTTTCTACAACCCGTTCCAGT
TCGAGATCACCTTCGAGTGCATCGAGGACCTGTCTGAAGACTTGGAATGGAAAATTATCTATGTGGGCTC
TGCAGAAAGTGAAGAATACGATCAAGTTTTAGACTCTGTTTTAGTGGGTCCTGTTCCCGCAGGAAGGCAT
ATGTTTGTATTTCAGGCTGATGCACCTAATCCAGGACTCATTCCAGATGCAGATGCAGTAGGCGTAACTG
TTGTGCTAATTACTTGTACCTATCGAGGACAAGAATTTATTAGAGTTGGCTATTATGTAAATAATGAATA
TACTGAGACAGAATTAAGGGAAAATCCACCAGTAAAACCAGACTTTTCTAAGCTTCAAAGGAATATTTTG
GCATCTAATCCCAGGGTCACAAGATTCCACATTAATTGGGAAGATAACACAGAAAAACTGGAAGATGCAG
AGAGCAGTAATCCAAATCTACAGTCACTTCTTTCAACAGATGCATTACCTTCAGCATCAAAGGGATGGTC
CACATCAGAAAACTCACTAAATGTCATGTTAGAATCCCACATGGACTGCATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200324 protein sequence
Red=Cloning site Green=Tags(s)

MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRH
MFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNIL
ASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014034
ORF Size 612 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014034.3
RefSeq Size 2525 bp
RefSeq ORF 615 bp
Locus ID 25842
UniProt ID Q9Y294
Cytogenetics 6q22.31
Domains Anti-silence
MW 23 kDa
Summary This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. provided by RefSeq, Jul 2008
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"NM_014034" in other vectors (6)
SKU Description Size Price
RC200324L1 Lenti ORF clone of Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A), Myc-DDK-tagged 10 ug
$600.00
RC200324L2 Lenti ORF clone of Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A), mGFP tagged 10 ug
$600.00
RC200324L3 Lenti ORF clone of Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A), Myc-DDK-tagged 10 ug
$600.00
RC200324L4 Lenti ORF clone of Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A), mGFP tagged 10 ug
$600.00
RG200324 ASF1A (tGFP-tagged) - Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A) 10 ug
$489.00 $500.00
SC321540 ASF1A (untagged)-Human ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A) 10 ug
$300.00

Plasmid Map for RC200324

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200324
Circular map for RC200324

file-alt Citations

Proteasomal-dependent CHK1 degradation leads to DNA damage accumulation in ALS cellular model systems
Modafferi, Silenzi, Garbelli et al
Cell Death Dis (2026)
AI Summary: OriGene’s Tagged ORF Clone (#RC200324) was used for transient overexpression experiments to restore CHK1 and ASF1A protein levels, enabling the study of their roles in reducing DNA damage in ALS cellular models.
Snippet: n Tagged ORF Clone (ORIGENE, #RC200324) or 1.5 µg of EV were transfected in each well. For sorting, cells were seeded in one 60 mm dish per condition. Transfection reactions were scaled up doubling every volume. In this case, pCS2plus -mCerulean
Proteasomal-dependent CHK1 degradation leads to DNA damage accumulation in ALS cellular model systems
Francia, Modafferi, Scarian et al
Research Square (2025)
AI Summary: OriGene’s ASF1A Human Tagged ORF Clone (#RC200324) was used for transient overexpression experiments to restore ASF1A protein levels and assess their impact on DNA damage accumulation in ALS cellular models.
Snippet: n empty vector (EV)) were transfected in each well. For overexpression of FUS P525L and ASF1A, 0.5 µg of FUS P525L mVenus, 0.5 µg of FUS P525L mCerulean and 1.5 µg of ASF1A Human Tagged ORF Clone (ORIGENE, #RC200324) or 1.5 µg of EV were transfected in each well. For sorting, cells were seeded in one 60 mm dish per condition. Transfection reactions were scaled up doubling every volume. In this
Showing 1-2 of 2 papers.
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