CEE (GET4) (NM_015949) Human Tagged ORF Clone
SKU
RC200220
GET4 (Myc-DDK-tagged)-Human golgi to ER traffic protein 4 homolog (S. cerevisiae) (GET4)
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CEE |
| Synonyms | C7orf20; CEE; CGI-20; TRC35 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_015949 |
| ORF Size | 981 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_015949.3 |
| RefSeq Size | 2106 bp |
| RefSeq ORF | 984 bp |
| Locus ID | 51608 |
| UniProt ID | Q7L5D6 |
| Cytogenetics | 7p22.3 |
| MW | 36.5 kDa |
| Summary | As part of a cytosolic protein quality control complex, the BAG6/BAT3 complex, maintains misfolded and hydrophobic patches-containing proteins in a soluble state and participates to their proper delivery to the endoplasmic reticulum or alternatively can promote their sorting to the proteasome where they undergo degradation (PubMed:20676083, PubMed:21636303, PubMed:21743475, PubMed:28104892). The BAG6/BAT3 complex is involved in the post-translational delivery of tail-anchored/type II transmembrane proteins to the endoplasmic reticulum membrane. Recruited to ribosomes, it interacts with the transmembrane region of newly synthesized tail-anchored proteins and together with SGTA and ASNA1 mediates their delivery to the endoplasmic reticulum (PubMed:20676083, PubMed:28104892, PubMed:25535373). Client proteins that cannot be properly delivered to the endoplasmic reticulum are ubiquitinated and sorted to the proteasome (PubMed:28104892). Similarly, the BAG6/BAT3 complex also functions as a sorting platform for proteins of the secretory pathway that are mislocalized to the cytosol either delivering them to the proteasome for degradation or to the endoplasmic reticulum (PubMed:21743475). The BAG6/BAT3 complex also plays a role in the endoplasmic reticulum-associated degradation (ERAD), a quality control mechanism that eliminates unwanted proteins of the endoplasmic reticulum through their retrotranslocation to the cytosol and their targeting to the proteasome. It maintains these retrotranslocated proteins in an unfolded yet soluble state condition in the cytosol to ensure their proper delivery to the proteasome (PubMed:21636303).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_015949" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200220L1 | Lenti ORF clone of Human golgi to ER traffic protein 4 homolog (S. cerevisiae) (GET4), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC200220L2 | Lenti ORF clone of Human golgi to ER traffic protein 4 homolog (S. cerevisiae) (GET4), mGFP tagged | 10 ug |
$600.00
|
|
| RC200220L3 | Lenti ORF clone of Human golgi to ER traffic protein 4 homolog (S. cerevisiae) (GET4), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC200220L4 | Lenti ORF clone of Human golgi to ER traffic protein 4 homolog (S. cerevisiae) (GET4), mGFP tagged | 10 ug |
$600.00
|
|
| RG200220 | GET4 (tGFP-tagged) - Human golgi to ER traffic protein 4 homolog (S. cerevisiae) (GET4) | 10 ug |
$500.00
|
|
| SC114540 | GET4 (untagged)-Human golgi to ER traffic protein 4 homolog (S. cerevisiae) (GET4) | 10 ug |
$300.00
|
|
Citations
BAG6 prevents the aggregation of neurodegeneration-associated fragments of TDP43
Kasu, Arva, Johnson et al
iScience (2022) 25 (5), 104273 DOI: 10.1016/j.isci.2022.104273
Kasu, Arva, Johnson et al
iScience (2022) 25 (5), 104273 DOI: 10.1016/j.isci.2022.104273
Cited by 28
AI Summary: In this study, OriGene’s pCMV6-Entry-based plasmids encoding myc-fTRC35 (Cat#RC200220) and myc-fUBL4A (Cat#RC208121) were used to investigate the roles of these proteins in the BAG6-mediated prevention of TDP43 fragment aggregation.
Snippet: ing Cre recombinase under the control of EF1α promoter Fenno et al., 2014 Addgene Cat#55636 p571: KanR;NeoR, pCMV6-Entry-based plasmid encoding myc-fTRC35 under the control of CMV promoter Origene Cat#RC200220 p572: KanR;NeoR, pCMV6-Entry-based plasmid encoding myc-fUBL4A under the control of CMV promoter Origene Cat#RC208121 p573: KanR;NeoR, pCMV6-Entry-based plasmid encodi
Showing 1-1 of 1 papers.
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