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RABL4 (IFT27) (NM_006860) Human Tagged ORF Clone

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SKU
RC200215
IFT27 (Myc-DDK-tagged)-Human intraflagellar transport 27 homolog (Chlamydomonas) (IFT27), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RABL4
Synonyms BBS19; FAP156; RABL4; RAYL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200215 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAAGCTGGCAGCCAAATGCATCCTGGCAGGAGACCCAGCAGTGGGCAAGACCGCCCTGGCACAGA
TCTTCCGCAGTGATGGAGCCCATTTCCAGAAAAGCTACACCCTGACAACAGGAATGGATTTGGTGGTGAA
GACAGTGCCAGTTCCTGACACGGGAGACAGTGTGGAACTCTTCATTTTTGACTCTGCTGGCAAGGAGCTG
TTTTCGGAAATGCTGGATAAATTGTGGGAGAGTCCCAATGTCTTATGTCTCGTCTATGATGTGACCAATG
AAGAATCCTTCAACAACTGCAGCAAGTGGCTGGAGAAGGCTCGGTCACAGGCTCCAGGCATCTCTCTCCC
AGGTGTTTTAGTTGGGAACAAGACAGACCTGGCCGGCAGACGAGCAGTGGACTCAGCTGAGGCCCGGGCA
TGGGCGCTGGGCCAGGGCCTGGAATGTTTTGAAACATCCGTGAAAGAGATGGAAAACTTCGAAGCCCCTT
TCCACTGCCTTGCCAAGCAGTTCCACCAGCTGTACCGGGAGAAGGTGGAGGTTTTCCGGGCCCTGGCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200215 protein sequence
Red=Cloning site Green=Tags(s)

MVKLAAKCILAGDPAVGKTALAQIFRSDGAHFQKSYTLTTGMDLVVKTVPVPDTGDSVELFIFDSAGKEL
FSEMLDKLWESPNVLCLVYDVTNEESFNNCSKWLEKARSQAPGISLPGVLVGNKTDLAGRRAVDSAEARA
WALGQGLECFETSVKEMENFEAPFHCLAKQFHQLYREKVEVFRALA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006860
ORF Size 558 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006860.5
RefSeq Size 1101 bp
RefSeq ORF 558 bp
Locus ID 11020
UniProt ID Q9BW83
Cytogenetics 22q12.3
Domains RAB, ras, RAS, RHO
Protein Families Druggable Genome
MW 20.5 kDa
Summary This gene encodes a GTP-binding protein that is a core component of the intraflagellar transport complex B. Characterization of the similar Chlamydomonas protein indicates a function in cell cycle control. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Jan 2012
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"NM_006860" in other vectors (4)
SKU Description Size Price
RC200215L3 Lenti ORF clone of Human intraflagellar transport 27 homolog (Chlamydomonas) (IFT27), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC200215L4 Lenti ORF clone of Human intraflagellar transport 27 homolog (Chlamydomonas) (IFT27), transcript variant 2, mGFP tagged 10 ug
$600.00
RG200215 IFT27 (tGFP-tagged) - Human intraflagellar transport 27 homolog (Chlamydomonas) (IFT27), transcript variant 2 10 ug
$489.00 $500.00
SC320196 IFT27 (untagged)-Human intraflagellar transport 27 homolog (Chlamydomonas) (IFT27), transcript variant 2 10 ug
$300.00

Plasmid Map for RC200215

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200215
Circular map for RC200215

file-alt Citations

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