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EXD2 (NM_018199) Human Tagged ORF Clone

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SKU
RC200127
EXD2 (Myc-DDK-tagged)-Human exonuclease 3'-5' domain containing 2 (EXD2), transcript variant 5
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EXD2
Synonyms C14orf114; EXDL2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200127 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCCCCAAGTGGCCTGTGTGTCTTGGTTCGCCTGCCCAAGCTAATCTGTGGAGGAAAAACACTAC
CAAGAACGTTATTGGATATTTTGGCAGATGGCACCATTTTGAAAGTTGGAGTGGGATGCTCAGAAGATGC
CAGCAAGCTTCTGCAGGATTATGGCCTCGTTGTTAGGGGGTGCCTGGACCTCCGATACCTAGCCATGCGG
CAGAGAAACAATTTGCTCTGTAATGGGCTTAGCCTGAAGTCCCTCGCTGAGACTGTTTTGAACTTTCCCC
TTGACAAGTCCCTTCTACTTCGTTGCAGCAACTGGGATGCTGAGACTCTCACAGAGGACCAGGTAATTTA
TGCTGCCAGGGATGCCCAGATTTCAGTGGCTCTCTTTCTTCATCTTCTTGGATACCCTTTCTCTAGGAAT
TCACCTGGAGAAAAAAACGATGACCACAGTAGCTGGAGAAAAGTCTTGGAAAAATGCCAGGGTGTGGTCG
ACATCCCATTTCGAAGCAAAGGAATGAGCAGATTGGGAGAAGAGGTTAATGGGGAAGCAACAGAATCTCA
GCAGAAGCCAAGAAATAAGAAGTCTAAGATGGATGGGATGGTGCCAGGCAACCACCAAGGGAGAGACCCC
AGAAAACATAAAAGAAAGCCTCTGGGGGTGGGCTATTCTGCCAGAAAATCACCTCTTTATGATAACTGCT
TTCTCCATGCTCCTGATGGACAGCCCCTCTGCACTTGTGATAGAAGAAAAGCTCAGTGGTACCTGGACAA
AGGCATTGGTGAGCTGGTGAGTGAAGAGCCCTTTGTGGTGAAGCTACGGTTTGAACCTGCAGGAAGGCCC
GAATCTCCTGGAGACTATTACTTGATGGTTAAAGAGAACCTGTGTGTAGTGTGTGGCAAGAGAGACTCCT
ACATTCGGAAGAACGTGATTCCACATGAGTACCGGAAGCACTTCCCCATCGAGATGAAGGACCACAACTC
CCACGATGTGCTGCTGCTCTGCACCTCCTGCCATGCCATTTCCAACTACTATGACAACCATCTGAAGCAG
CAGCTGGCCAAGGAGTTCCAGGCCCCCATCGGCTCTGAGGAGGGCTTGCGCCTGCTGGAAGATCCTGAGC
GCCGGCAGGTGCGTTCTGGGGCCAGGGCCCTGCTCAACGCGGAGAGCCTGCCTACTCAGCGAAAGGAGGA
GCTGCTGCAAGCACTCAGAGAGTTTTATAACACAGACGTGGTCACAGAGGAGATGCTTCAAGAGGCTGCC
AGCCTGGAGACCAGAATCTCCAATGAAAACTATGTTCCTCACGGGCTGAAGGTGGTGCAGTGTCACAGCC
AGGGTGGCCTGCGCTCCCTCATGCAGCTGGAGAGCCGCTGGCGTCAGCACTTCCTGGACTCCATGCAGCC
CAAGCACCTGCCCCAGCAGTGGTCAGTGGACCACAACCATCAGAAGCTGCTCCGGAAATTCGGGGAAGAT
CTTCCCATCCAGCTGTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200127 protein sequence
Red=Cloning site Green=Tags(s)

MASPSGLCVLVRLPKLICGGKTLPRTLLDILADGTILKVGVGCSEDASKLLQDYGLVVRGCLDLRYLAMR
QRNNLLCNGLSLKSLAETVLNFPLDKSLLLRCSNWDAETLTEDQVIYAARDAQISVALFLHLLGYPFSRN
SPGEKNDDHSSWRKVLEKCQGVVDIPFRSKGMSRLGEEVNGEATESQQKPRNKKSKMDGMVPGNHQGRDP
RKHKRKPLGVGYSARKSPLYDNCFLHAPDGQPLCTCDRRKAQWYLDKGIGELVSEEPFVVKLRFEPAGRP
ESPGDYYLMVKENLCVVCGKRDSYIRKNVIPHEYRKHFPIEMKDHNSHDVLLLCTSCHAISNYYDNHLKQ
QLAKEFQAPIGSEEGLRLLEDPERRQVRSGARALLNAESLPTQRKEELLQALREFYNTDVVTEEMLQEAA
SLETRISNENYVPHGLKVVQCHSQGGLRSLMQLESRWRQHFLDSMQPKHLPQQWSVDHNHQKLLRKFGED
LPIQLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018199
ORF Size 1488 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018199.3
RefSeq Size 4568 bp
RefSeq ORF 1491 bp
Locus ID 55218
UniProt ID Q9NVH0, Q9NVH0-2
Cytogenetics 14q24.1
Domains 3_5_exonuclease
MW 56.3 kDa
Summary Exonuclease required for double-strand breaks resection and efficient homologous recombination. Plays a key role in controlling the initial steps of chromosomal break repair, it is recruited to chromatin in a damage-dependent manner and functionally interacts with the MRN complex to accelerate resection through its 3'-5' exonuclease activity, which efficiently processes double-stranded DNA substrates containing nicks.UniProtKB/Swiss-Prot Function
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"NM_018199" in other vectors (6)
SKU Description Size Price
RC200127L1 Lenti ORF clone of Human exonuclease 3'-5' domain containing 2 (EXD2), transcript variant 5, Myc-DDK-tagged 10 ug
$757.00
RC200127L2 Lenti ORF clone of Human exonuclease 3'-5' domain containing 2 (EXD2), transcript variant 5, mGFP tagged 10 ug
$757.00
RC200127L3 Lenti ORF clone of Human exonuclease 3'-5' domain containing 2 (EXD2), transcript variant 5, Myc-DDK-tagged 10 ug
$757.00
RC200127L4 Lenti ORF clone of Human exonuclease 3'-5' domain containing 2 (EXD2), transcript variant 5, mGFP tagged 10 ug
$757.00
RG200127 EXD2 (tGFP-tagged) - Human exonuclease 3'-5' domain containing 2 (EXD2), transcript variant 5 10 ug
$489.00 $657.00
SC128176 EXD2 (untagged)-Human exonuclease 3'-5' domain containing 2 (EXD2), transcript variant 5 10 ug
$457.00

Plasmid Map for RC200127

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200127
Circular map for RC200127

file-alt Citations

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