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DHFR (NM_000791) Human Tagged ORF Clone

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SKU
RC200089
DHFR (Myc-DDK-tagged)-Human dihydrofolate reductase (DHFR)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DHFR
Synonyms DHFRP1; DYR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC200089 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTGGTTCGCTAAACTGCATCGTCGCTGTGTCCCAGAACATGGGCATCGGCAAGAACGGGGACCTGC
CCTGGCCACCGCTCAGGAATGAATTCAGATATTTCCAGAGAATGACCACAACCTCTTCAGTAGAAGGTAA
ACAGAATCTGGTGATTATGGGTAAGAAGACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGGT
AGAATTAATTTAGTTCTCAGCAGAGAACTCAAGGAACCTCCACAAGGAGCTCATTTTCTTTCCAGAAGTC
TAGATGATGCCTTAAAACTTACTGAACAACCAGAATTAGCAAATAAAGTAGACATGGTCTGGATAGTTGG
TGGCAGTTCTGTTTATAAGGAAGCCATGAATCACCCAGGCCATCTTAAACTATTTGTGACAAGGATCATG
CAAGACTTTGAAAGTGACACGTTTTTTCCAGAAATTGATTTGGAGAAATATAAACTTCTGCCAGAATACC
CAGGTGTTCTCTCTGATGTCCAGGAGGAGAAAGGCATTAAGTACAAATTTGAAGTATATGAGAAGAATGA
T

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC200089 protein sequence
Red=Cloning site Green=Tags(s)

MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKG
RINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIM
QDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000791
ORF Size 561 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000791.4
RefSeq Size 3932 bp
RefSeq ORF 564 bp
Locus ID 1719
UniProt ID P00374
Cytogenetics 5q14.1
Domains DiHfolate_red
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Folate biosynthesis, Metabolic pathways, One carbon pool by folate
MW 21.5 kDa
Summary Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Mar 2014
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"NM_000791" in other vectors (6)
SKU Description Size Price
RC200089L1 Lenti ORF clone of Human dihydrofolate reductase (DHFR), Myc-DDK-tagged 10 ug
$600.00
RC200089L2 Lenti ORF clone of Human dihydrofolate reductase (DHFR), mGFP tagged 10 ug
$600.00
RC200089L3 Lenti ORF clone of Human dihydrofolate reductase (DHFR), Myc-DDK-tagged 10 ug
$600.00
RC200089L4 Lenti ORF clone of Human dihydrofolate reductase (DHFR), mGFP tagged 10 ug
$600.00
RG200089 DHFR (tGFP-tagged) - Human dihydrofolate reductase (DHFR) 10 ug
$489.00 $500.00
SC321334 DHFR (untagged)-Human dihydrofolate reductase (DHFR) 10 ug
$300.00

Plasmid Map for RC200089

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200089
Circular map for RC200089

file-alt Citations

DHFR/TYMS are positive regulators of glioma cell growth and modulate chemo-sensitivity to temozolomide
Zhao, Tan, Dai et al
Eur. J. Pharmacol. (2019) 863, 172665 DOI: 10.1016/j.ejphar.2019.172665
AI Summary: OriGene products, including wild type DHFR and TYMS expression plasmids, DHFR and TYMS siRNA, and AMPKa shRNA, were used in this study to manipulate gene expression and investigate the roles of these genes in glioma cell growth and sensitivity to temozolomide.
Snippet: 2.5. Plasmids and transfection. The plasmids that express wild type DHFR or TYMS, DHFR, TYMS siRNA and AMPKa shRNA (all were purchased from Origene) were transfected into cells by Lipofectamine (2000) (Invitrogen) according to the manufacturer's protocol;
NADPH production by the oxidative pentose-phosphate pathway supports folate metabolism
Chen, Zhang, Hoshino et al
Nat Metab (2019) 1, 404-415
AI Summary: OriGene Technologies contributed to this study by providing the Human DHFR cDNA (product RC200089), which was used for recombinant expression experiments to assess the role of DHFR in folate metabolism.
Snippet: LentiCRISPR v2 (52961) and GeCKOv2 in lentiCRISPRv2 (1000000048), and plasmid containing E.coli DHFR sequence (20214) were purchased from Addgene (Cambridge, MA). cDNA of G6PD (MHS6278-202826068) was from Dharmacon Inc. cDNA of Human DHFR (RC200089) was purchased from OriGene Technologies. All primers were synthesized by IDT (Coralvi, IA). Standard laboratory chemicals were from Sigma
FigureFigure
Showing 1-2 of 2 papers.
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