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DHFR (NM_000791) Human Untagged Clone

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SKU
SC321334
DHFR (untagged)-Human dihydrofolate reductase (DHFR)
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DHFR
Synonyms DHFRP1; DYR
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_000791.3 CTTGACCGCGCGTTCTGCTGTAACGAGCGGGCTCGGAGGTCCTCCCGCTGCTGTCATGGT
TGGTTCGCTAAACTGCATCGTCGCTGTGTCCCAGAACATGGGCATCGGCAAGAACGGGGA
CCTGCCCTGGCCACCGCTCAGGAATGAATTCAGATATTTCCAGAGAATGACCACAACCTC
TTCAGTAGAAGGTAAACAGAATCTGGTGATTATGGGTAAGAAGACCTGGTTCTCCATTCC
TGAGAAGAATCGACCTTTAAAGGGTAGAATTAATTTAGTTCTCAGCAGAGAACTCAAGGA
ACCTCCACAAGGAGCTCATTTTCTTTCCAGAAGTCTAGATGATGCCTTAAAACTTACTGA
ACAACCAGAATTAGCAAATAAAGTAGACATGGTCTGGATAGTTGGTGGCAGTTCTGTTTA
TAAGGAAGCCATGAATCACCCAGGCCATCTTAAACTATTTGTGACAAGGATCATGCAAGA
CTTTGAAAGTGACACGTTTTTTCCAGAAATTGATTTGGAGAAATATAAACTTCTGCCAGA
ATACCCAGGTGTTCTCTCTGATGTCCAGGAGGAGAAAGGCATTAAGTACAAATTTGAAGT
ATATGAGAAGAATGATTAATATGAAGGTGTTTTCTAGTTTAAGTTGTTCCCCCTCCCTCT
GAAAAAAGTATGTATTTTTACATTAGAAAAGGTTTTTTGTTGACTTTAGATCTATAATTA
TTTCTAAGCAACTAGTTTTTATTCCCCACTACTCTTGTCTCTATCAGATACCATTTATGA
GACATTCTTGCTATAACTAAGTGCTTCTCCAAGACCCCAACTGAGTCCCCAGCACCTGCT
ACAGTGAGCTGCCATTCCACACCCATCACATGTGGCACTCTTGCCAGTCCTTGACATTGT
CGGGCTTTTCACATGTTGGTAATATTTATTAAAGATGAAGATCCACATACCCTTCAACTG
AGCAGTTTCACTAGTGGAAATACCAAAAGCTTCCTACGTGTATATCCAGAGGTTTGTAGA
TAAATGTTGCCACCTTGTTTGTAACAGTGAAAAATTGAAAACAACCTGGAAGTCCAGTGA
TGGGAAAATGAGTATGTTTCTGTCTTAGATTGGGGAACCCAAAGCAGATTGCAAGACTGA
AATTTCAGTGAAAGCAGTGTATTTGCTAGGTCATACCAGAAATCATCAATTGAGGTACGG
AGAAACTGAACTGAGAAGGTAAGAAAAGCAATTTAAAGTCAGCGAGCAGGTTCTCATTGA
TAACAAGCTCCATACTGCTGAGATACAGGGAAATGGAGGGGGGAAAGCTGGAGTATTGAT
CCCGCCCCCCTCCTTGGTTGTCAGCTCCCTGTCCTGTGTGTGGGCGGAACATAGTCCAGC
TGCTCTATAGCAAGTCTCAGGTGTTTGCAGTAAGAAGCTGCTGGCATGCACGGGAACAGT
GAATGCCAAACACTTAAAGCAATTCGATGTTTAAGTATGTAAGTTCTTTTTTTTTTAGAC
AGCGTTTCGCTCTTGTTGCCCAGGCTGGCATGCAGTGGTGTGACCTCGGCTTACTGCAAC
CTCCGCCTTCCCAGATTCAAGCGATTCTCCTGCCTCAGGCTCCCAAGTAGCTAGGACCAG
GTGCGCGCCACCACGCCCGGCTAATTTTTGTATTTTGTATTTTTAGTAGAGATGGGGTTT
CACCATGTTGGTCAGGCTGGTCTCGAACTCGTGACCGCAAGCGATTCACCCACCTCAGCC
TCCCAAAGTGCTGGGATTACCGGCTTGAGCCACCACACCCGGCACATCTTCATTCTTTTT
ATGTAGTAAAAAGTATAAGGCCACACATGGTTTATTTGAAGTATTTTATAATTTAAAAAA
ATACAGAAGCAGGAAAACCAATTATAAGTTCAAGTGAGGGATGATGGTTGCTTGAACCAA
AGGGTTGCATGTAGTAAGAAATTGTGATTTAAGATATATTTTAAAGTTATAAGTAGCAGG
ATATTCTGATGGAGTTTGACTTTGGTTTTGGGCCCAGGGAGTTTCAGATGCCTTTGAGAA
ATGAATGAAGTAGAGAGAAAATAAAAGAAAAACCAGCCAGGCACAGTGGCTCACGCCTGT
AATCCCAGCGCTTTGGGAGGCTAAGGCAGGCAGATCACTTGAGACCAGCTTGGGCAACAT
GGCAAAGCCCCATCTCTACAAAAAACACAAAAATTAGCTGGGCATTGTGGCGCACACCTG
TATTCCCATCTAGTCAGGAAGCTGAGATGGAAGAATTAATTGAGCCCACGAGTTCAAGGC
TGCAGTGAGTCGTGATTGTGCCACTGCACTCCAGCCGGGGTGACAGAAGAGACCTTGTCT
CGAAAAGGAATCTGAAAACAATGGAACCATGCCTTCATAATTCTAGAAAGTTATTTTCAA
CTGATAAATCTATATTCACCCAAATAATCAAGGGTGAAGGTAAAATAATACATTTTTAGA
CAAGCAAAGACTCAGGGGTTACCTCCATGTGCCCTTTTTAGGGAAGCTGTTGGAGAAAAT
ACTCCAGCAAAATGAAGGAGTACACAAACCAGAGAATGACATGAATCCAGCAAATAGGAT
CCAACACAGGCAATATTCCAGCTATGGAGCTAGCTTTAAAAAGGAACAGTAAAAATATTA
ATCGGTTAGCTGGGTGGAATGGCCCATGCCTGTAGTCCCAGCTACTCAGGAGGCTCAGCA
GCAGGACGACTTGAGCCCAAGAGTTCCAGACCAGCCTGGCCACCTTAGTGAGATCCCTTC
TCTTAAAAATAATAACTTATTGCCAGATTTGGGGCATTTGGAAAGAAGTTCATTGAAGAT
AAAGCAAAAGTAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_000791
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000791.3, NP_000782.1
RefSeq Size 3932 bp
RefSeq ORF 564 bp
Locus ID 1719
UniProt ID P00374
Cytogenetics 5q14.1
Domains DiHfolate_red
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Folate biosynthesis, Metabolic pathways, One carbon pool by folate
Summary Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Mar 2014
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
Resources
"NM_000791" in other vectors (6)
SKU Description Size Price
RC200089 DHFR (Myc-DDK-tagged)-Human dihydrofolate reductase (DHFR) 10 ug
$300.00
RC200089L1 Lenti ORF clone of Human dihydrofolate reductase (DHFR), Myc-DDK-tagged 10 ug
$600.00
RC200089L2 Lenti ORF clone of Human dihydrofolate reductase (DHFR), mGFP tagged 10 ug
$600.00
RC200089L3 Lenti ORF clone of Human dihydrofolate reductase (DHFR), Myc-DDK-tagged 10 ug
$600.00
RC200089L4 Lenti ORF clone of Human dihydrofolate reductase (DHFR), mGFP tagged 10 ug
$600.00
RG200089 DHFR (tGFP-tagged) - Human dihydrofolate reductase (DHFR) 10 ug
$489.00 $500.00

file-alt Citations

Disparate Response to Methotrexate in Stem Versus Non-Stem Cells
Beane, Darling, Fonseca et al
Stem Cell Rev (2016)
Cited by 11
AI Summary: OriGene’s Human DHFR cDNA clone (SC321334) was used in this study to overexpress DHFR in cells, enabling the authors to investigate how increased DHFR levels affect cell response to methotrexate treatment.
Snippet: Human DHFR cDNA (ORIGENE clone SC321334, Ref Seq: NM_000791.3) was inserted into the multiple cloning site of pEmGFP-N1 in frame with EmGFP via PCR-based cloning using Q5 high fidelity polymerase (New England BioLabs Inc.). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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