LDL Receptor (LDLR) (NM_000527) Human Tagged ORF Clone
SKU
RC200006
LDLR (Myc-DDK-tagged)-Human low density lipoprotein receptor (LDLR), transcript variant 1
$1,181.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | LDL Receptor |
| Synonyms | FH; FHC; FHCL1; LDLCQ2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000527 |
| ORF Size | 2580 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000527.2, NP_000518.1 |
| RefSeq Size | 5175 bp |
| RefSeq ORF | 2583 bp |
| Locus ID | 3949 |
| UniProt ID | P01130 |
| Cytogenetics | 19p13.2 |
| Domains | EGF, EGF_CA, ldl_recept_a, ldl_recept_b |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Endocytosis |
| MW | 95.38 kDa |
| Summary | The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.provided by RefSeq, Sep 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000527" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC200006L1 | Lenti ORF clone of Human low density lipoprotein receptor (LDLR), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,481.00
|
|
| RC200006L2 | Lenti ORF clone of Human low density lipoprotein receptor (LDLR), transcript variant 1, mGFP tagged | 10 ug |
$1,481.00
|
|
| RC200006L3 | Lenti ORF clone of Human low density lipoprotein receptor (LDLR), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,481.00
|
|
| RC200006L4 | Lenti ORF clone of Human low density lipoprotein receptor (LDLR), transcript variant 1, mGFP tagged | 10 ug |
$1,481.00
|
|
| RG200006 | LDLR (tGFP-tagged) - Human low density lipoprotein receptor (LDLR), transcript variant 1 | 10 ug |
$1,381.00
|
|
| SC110941 | LDLR (untagged)-Human low density lipoprotein receptor (LDLR), transcript variant 1 | 10 ug |
$1,182.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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