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LDL Receptor (LDLR) (NM_000527) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC200006L2
Lenti ORF clone of Human low density lipoprotein receptor (LDLR), transcript variant 1, mGFP tagged
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,481.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LDL Receptor
Synonyms FH; FHC; FHCL1; LDLCQ2
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC200006).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000527
ORF Size 2580 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000527.2, NP_000518.1
RefSeq Size 5175 bp
RefSeq ORF 2583 bp
Locus ID 3949
UniProt ID P01130
Cytogenetics 19p13.2
Domains EGF, EGF_CA, ldl_recept_a, ldl_recept_b
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Endocytosis
MW 95.38 kDa
Summary The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.provided by RefSeq, Sep 2010
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"NM_000527" in other vectors (6)
SKU Description Size Price
RC200006 LDLR (Myc-DDK-tagged)-Human low density lipoprotein receptor (LDLR), transcript variant 1 10 ug
$1,181.00
RC200006L1 Lenti ORF clone of Human low density lipoprotein receptor (LDLR), transcript variant 1, Myc-DDK-tagged 10 ug
$1,481.00
RC200006L3 Lenti ORF clone of Human low density lipoprotein receptor (LDLR), transcript variant 1, Myc-DDK-tagged 10 ug
$1,481.00
RC200006L4 Lenti ORF clone of Human low density lipoprotein receptor (LDLR), transcript variant 1, mGFP tagged 10 ug
$1,481.00
RG200006 LDLR (tGFP-tagged) - Human low density lipoprotein receptor (LDLR), transcript variant 1 10 ug
$1,381.00
SC110941 LDLR (untagged)-Human low density lipoprotein receptor (LDLR), transcript variant 1 10 ug
$1,182.00

Plasmid Map for RC200006L2

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC200006L2
Circular map for RC200006L2

file-alt Citations

Targeting lipid droplet lysophosphatidylcholine for cisplatin chemotherapy
Chen, Ma, Cheng et al
J. Cell. Mol. Med. (2020)
Cited by 17
AI Summary: OriGene’s pLenti‐C‐mGFP‐LDLR (RC200006L2) plasmid was used to generate LDLR overexpression cell lines, enabling the study of LDLR’s role in lipid metabolism and cisplatin resistance in cancer cells.
Snippet: LDLR knockdown cells or overexpression clone cells were engineered by the stable transfection of human LDLR cDNA (pLenti‐C‐mGFP‐LDLR, RC200006L2;OriGENE) or pLKO.1‐shLDLR (targeting sequence: 5′‐GGG CGA CAG ATG CGA AAG AAA)10 and then;- LDLR knockdown cells or overexpression clone cells were engineered by the stable transfection of human LDLR cDNA (pLenti‐C‐mGFP‐LDLR, RC200006L2
Low-Density Lipoprotein Receptor-Mediated Lipidome-Transcriptome Reprogramming Impulses to Cisplatin Insensitivity
Chang, Wang, Cheng et al
bioRxiv (2018)
Snippet: LDLR knockdown or overexpression clones were engineered by the stable transfection of human LDLR cDNA (pLenti-C-mGFP-LDLR, RC200006L2;OriGENE, Rockville, MD, USA) or pLKO.1-shLDLR (targeting sequence shown in Expanded Table 6) and then selected after exposure to puromycin (10 μM) for a period of time. The pLKO-shLuciferase, shLDLR, shFAM83B (Expanded Table 6) plasmids were obtained from the National RNAi Core Facility Platform (Institute of Molecular Biology/Genome Research Center, Academia Sinica
Showing 1-2 of 2 papers.
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