Tle4 (NM_001302947) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR231124
Tle4 (myc-DDK-tagged) - Mouse transducin-like enhancer of split 4, homolog of Drosophila E(spl) (Tle4), transcript variant 1
$589.00
$709.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Tle4 |
| Synonyms | 5730411M05Rik; AA792082; Bce-1; Bce1; ESTM13; ESTM14; grg-4; Grg4; X83332; X83333 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001302947 |
| ORF Size | 2322 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001302947.1, NP_001289876.1 |
| RefSeq Size | 4758 bp |
| RefSeq ORF | 2325 bp |
| Locus ID | 21888 |
| Cytogenetics | 19 9.11 cM |
| MW | 84.4 kDa |
| Summary | Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001302947" in other vectors (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC228913 | Tle4 (untagged) - Mouse transducin-like enhancer of split 4, homolog of Drosophila E(spl) (Tle4), transcript variant 1 | 10 ug |
$781.00
|
|
Citations
Transcriptional repression by FEZF2 restricts alternative identities of cortical projection neurons
Tsyporin, Tastad, Ma et al
Cell Rep (2021) 35 (12), 109269 DOI: 10.1016/j.celrep.2021.109269
Tsyporin, Tastad, Ma et al
Cell Rep (2021) 35 (12), 109269 DOI: 10.1016/j.celrep.2021.109269
Cited by 53
AI Summary: In this study, the OriGene product pCMV-Tle4-Myc-DDK tag (OriGene #MR231124) was used to investigate the role of TLE4 in FEZF2-mediated transcriptional repression that regulates cortical projection neuron subtype identities.
Snippet: a Cruz pCAG-Fezf2-EnR This Paper N/A pCAG-Fezf2-VP16 This Paper N/A pCAG-EGFP Matsuda and Cepko, 2004 Addgene #11150 pCMV-Tle4-Myc-DDK tag This paper Origene #MR231124 Software and algorithms FIJI Schindelin et al., 2012 https://imagej.net/Fiji Adobe Illustrator Adobe https://www.adobe.com/products/illustrator.html
Showing 1-1 of 1 papers.
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