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Tle4 (NM_001302947) Mouse Untagged Clone

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SKU
MC228913
Tle4 (untagged) - Mouse transducin-like enhancer of split 4, homolog of Drosophila E(spl) (Tle4), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $781.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tle4
Synonyms 5730411M05Rik; AA792082; Bce-1; Bce1; ESTM13; ESTM14; grg-4; Grg4; X83332; X83333
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC228913 representing NM_001302947
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTCGCGACCTGAGCAAGATGTACCCGCAGACGCGCCACCCGGCACCGCATCAGCCTGCTCAACCCT
TCAAATTTACAATTTCAGAATCCTGTGATCGGATTAAGGAAGAGTTTCAGTTTTTACAGGCTCAATACCA
CAGTCTGAAGCTGGAATGTGAGAAGCTCGCCAGCGAGAAGACAGAGATGCAGCGGCATTATGTCATGTAT
TATGAAATGTCCTATGGGTTGAACATAGAAATGCACAAGCAGGCAGAGATTGTTAAACGACTAAATGCTA
TCTGTGCACAGGTCATTCCTTTCCTGTCCCAAGAGCACCAGCAACAAGTGGTGCAGGCTGTGGAACGGGC
CAAGCAGGTGACCATGGCAGAACTGAACGCCATCATTGGGCAGCAACAACTCCAAGCTCAGCATTTATCA
CATGGACATGGTCTGCCTGTGCCTCTGACACCACACCCTTCAGGGCTTCAGCCCCCAGCCATCCCACCTA
TCGGTAGCAGTGCAGGACTTCTGGCCCTCTCCAGTGCACTAGGAGGTCAGTCTCACCTCCCAATTAAGGA
TGAGAAGAAGCACCATGACAATGATCACCAAAGAGACAGAGACTCCATCAAGAGCTCTTCCGTATCCCCA
TCAGCCAGTTTCCGGGGTTCTGAGAAACATAGGAACTCTACAGACTATTCCTCAGAGAGCAAAAAGCAGA
AAACAGAAGAAAAAGAAATTGCAGCTCGCTATGACAGTGATGGTGAGAAGAGTGATGACAACTTGGTAGT
TGATGTGTCCAATGAGGATCCATCTTCCCCCCGAGGAAGCCCAGCACATTCCCCAAGGGAGAATGGCCTG
GACAAGACACGACTCCTCAAGAAAGATGCCCCCATCAGCCCCGCTTCTGTTGCATCTTCCAGCAGTACTC
CTTCCTCCAAATCCAAAGAGCTTAGCCTTAATGAAAAGTCTACTACTCCTGTTTCAAAGTCCAATACCCC
TACTCCACGAACTGATGCACCTACCCCTGGCAGTAATTCTACTCCTGGACTGAGGCCTGTACCTGGAAAG
CCACCAGGTGTGGACCCCTTAGCATCAAGCCTGAGGACCCCGATGGCTGTACCTTGTCCATATCCAACCC
CCTTTGGGATCGTGCCCCATGCTGGGATGAACGGAGAGCTGACCAGCCCCGGAGCCGCCTATGCTGGGCT
ACACAACATCTCCCCCCAGATGAGTGCAGCTGCTGCTGCAGCTGCGGCAGCAGCAGCCTATGGAAGATCA
CCTGTGGTGGGATTTGATCCACACCATCACATGCGAGTGCCAGCAATACCTCCAAATCTAACAGGCATTC
CAGGAGGAAAACCAGCATACTCCTTTCACGTCAGTGCCGATGGCCAGATGCAGCCTGTCCCTTTCCCCCC
TGATGCCCTCATTGGACCAGGGATCCCCCGACACGCTCGACAGATCAACACCCTCAACCATGGGGAGGTG
GTGTGTGCAGTGACCATCAGCAACCCCACAAGGCACGTGTACACAGGTGGCAAGGGCTGTGTTAAGGTCT
GGGACATCAGCCACCCTGGCAACAAGAGCCCAGTCTCTCAGCTGGACTGTCTGAACAGGGATAACTACAT
CCGTTCCTGCAGATTGCTCCCTGATGGTCGCACCTTAATTGTTGGAGGGGAAGCCAGCACACTGTCCATC
TGGGACCTGGCAGCTCCAACTCCACGCATCAAGGCAGAGCTGACATCCTCAGCCCCTGCCTGCTATGCTC
TGGCCATCAGCCCCGACTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGTAACATCGCAGTGTGGGATCT
GCACAACCAGACTCTGGTGAGGCAATTCCAGGGGCACACAGATGGAGCCAGCTGTATTGACATTTCTAAT
GATGGCACCAAGCTCTGGACAGGTGGTTTGGACAACACTGTGAGGTCCTGGGACCTGCGTGAAGGGCGGC
AGCTGCAGCAACATGACTTCACCTCTCAGATCTTTTCATTGGGCTATTGCCCAACTGGAGAGTGGCTTGC
AGTGGGGATGGAGAATAGCAATGTGGAAGTATTGCATGTCACCAAACCAGACAAATACCAGTTGCATCTT
CATGAGAGCTGTGTGCTGTCACTCAAGTTTGCCCACTGTGGCAAATGGTTTGTAAGCACTGGAAAGGACA
ACCTTCTGAATGCTTGGAGGACGCCTTATGGGGCCAGCATATTCCAGTCCAAAGAATCCTCATCGGTGCT
TAGCTGTGACATCTCTGTGGATGACAAGTACATTGTCACTGGCTCTGGGGACAAGAAAGCTACGGTTTAT
GAAGTTATTTATTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001302947
Insert Size 2325 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001302947.1, NP_001289876.1
RefSeq Size 4758 bp
RefSeq ORF 2325 bp
Locus ID 21888
Cytogenetics 19 9.11 cM
Summary Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
Resources
"NM_001302947" in other vectors (1)
SKU Description Size Price
MR231124 Tle4 (myc-DDK-tagged) - Mouse transducin-like enhancer of split 4, homolog of Drosophila E(spl) (Tle4), transcript variant 1 10 ug
$589.00 $709.00

file-alt Citations

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