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Stat1 (NM_009283) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR227437
Stat1 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 1 (Stat1), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $1,029.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Stat1
Synonyms 2010005J02Rik; AA408197
Vector pCMV6-Entry2
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR227437 representing NM_009283
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCACAGTGGTTCGAGCTTCAGCAGCTGGACTCCAAGTTCCTGGAGCAGGTCCACCAGCTGTACGATG
ACAGTTTCCCCATGGAAATCAGACAGTACCTGGCCCAGTGGCTGGAAAAGCAAGACTGGGAGCACGCTGC
CTATGATGTCTCGTTTGCGACCATCCGCTTCCATGACCTCCTCTCACAGCTGGACGACCAGTACAGCCGC
TTTTCTCTGGAGAATAATTTCTTGTTGCAGCACAACATACGGAAAAGCAAGCGTAATCTCCAGGATAACT
TCCAAGAAGATCCCGTACAGATGTCCATGATCATCTACAACTGTCTGAAGGAAGAAAGGAAGATTTTGGA
AAATGCCCAAAGATTTAATCAGGCCCAGGAGGGAAATATTCAGAACACTGTGATGTTAGATAAACAGAAG
GAGCTGGACAGTAAAGTCAGAAATGTGAAGGATCAAGTCATGTGCATAGAGCAGGAAATCAAGACCCTAG
AAGAATTACAAGATGAATATGACTTTAAATGCAAAACCTCTCAGAACAGAGAAGGTGAAGCCAATGGTGT
GGCGAAGAGCGACCAAAAACAGGAACAGCTGCTGCTCCACAAGATGTTTTTAATGCTTGACAATAAGAGA
AAGGAGATAATTCACAAAATCAGAGAGTTGCTGAATTCCATCGAGCTCACTCAGAACACTCTGATTAATG
ACGAGCTCGTGGAGTGGAAGCGAAGGCAGCAGAGCGCCTGCATCGGGGGACCGCCCAACGCCTGCCTGGA
TCAGCTGCAAAGCTGGTTCACCATTGTTGCAGAGACCCTGCAGCAGATCCGTCAGCAGCTTAAAAAGCTG
GAGGAGTTGGAACAGAAATTCACCTATGAGCCCGACCCTATTACAAAAAACAAGCAGGTGTTGTCAGATC
GAACCTTCCTCCTCTTCCAGCAGCTCATTCAGAGCTCCTTCGTGGTAGAACGACAGCCGTGCATGCCCAC
TCACCCGCAGAGGCCCCTGGTCTTGAAGACTGGGGTACAGTTCACTGTCAAGCTGAGACTGTTGGTGAAA
TTGCAAGAGCTGAACTATAACTTGAAAGTGAAAGTCTCATTTGACAAAGATGTGAACGAGAAAAACACAG
TTAAAGGATTTCGGAAGTTCAACATCTTGGGTACGCACACAAAAGTGATGAACATGGAAGAATCCACCAA
CGGAAGTCTGGCAGCTGAGTTCCGACACCTGCAACTGAAGGAACAGAAAAACGCTGGGAACAGAACTAAT
GAGGGGCCTCTCATTGTCACCGAAGAACTTCACTCTCTTAGCTTTGAAACCCAGTTGTGCCAGCCAGGCT
TGGTGATTGACCTGGAGACCACCTCTCTTCCTGTCGTGGTGATCTCCAACGTCAGCCAGCTCCCCAGTGG
CTGGGCGTCTATCCTGTGGTACAACATGCTGGTGACAGAGCCCAGGAATCTCTCCTTCTTCCTGAACCCC
CCGTGCGCGTGGTGGTCCCAGCTCTCAGAGGTGTTGAGTTGGCAGTTTTCATCAGTCACCAAGAGAGGTC
TGAACGCAGACCAGCTGAGCATGCTGGGAGAGAAGCTGCTGGGCCCTAATGCTGGCCCTGATGGTCTTAT
TCCATGGACAAGGTTTTGTAAGGAAAATATTAATGATAAAAATTTCTCCTTCTGGCCTTGGATTGACACC
ATCCTAGAGCTCATTAAGAAGCACCTGCTGTGCCTCTGGAATGATGGGTGCATTATGGGCTTCATCAGCA
AGGAGCGAGAACGCGCTCTGCTCAAGGACCAGCAGCCAGGGACGTTCCTGCTTAGATTCAGTGAGAGCTC
CCGGGAAGGGGCCATCACATTCACATGGGTGGAACGGTCCCAGAACGGAGGTGAACCTGACTTCCATGCC
GTGGAGCCCTACACGAAAAAAGAACTTTCAGCTGTTACTTTCCCAGATATTATTCGCAACTACAAAGTCA
TGGCTGCCGAGAACATACCAGAGAATCCCCTGAAGTATCTGTACCCCAATATTGACAAAGACCACGCCTT
TGGGAAGTATTATTCCAGACCAAAGGAAGCACCAGAACCGATGGAGCTTGACGACCCTAAGCGAACTGGA
TACATCAAGACTGAGTTGATTTCTGTGTCTGAAGTCCACCCTTCTAGACTTCAGACCACAGACAACCTGC
TTCCCATGTCTCCAGAGGAGTTTGATGAGATGTCCCGGATAGTGGGCCCCGAATTTGACAGTATGATGAG
CACAGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR227437 representing NM_009283
Red=Cloning site Green=Tags(s)

MSQWFELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAAYDVSFATIRFHDLLSQLDDQYSR
FSLENNFLLQHNIRKSKRNLQDNFQEDPVQMSMIIYNCLKEERKILENAQRFNQAQEGNIQNTVMLDKQK
ELDSKVRNVKDQVMCIEQEIKTLEELQDEYDFKCKTSQNREGEANGVAKSDQKQEQLLLHKMFLMLDNKR
KEIIHKIRELLNSIELTQNTLINDELVEWKRRQQSACIGGPPNACLDQLQSWFTIVAETLQQIRQQLKKL
EELEQKFTYEPDPITKNKQVLSDRTFLLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVK
LQELNYNLKVKVSFDKDVNEKNTVKGFRKFNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQKNAGNRTN
EGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVTEPRNLSFFLNP
PCAWWSQLSEVLSWQFSSVTKRGLNADQLSMLGEKLLGPNAGPDGLIPWTRFCKENINDKNFSFWPWIDT
ILELIKKHLLCLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHA
VEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDDPKRTG
YIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEMSRIVGPEFDSMMSTV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_009283
ORF Size 2247 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009283.4
RefSeq Size 5217 bp
RefSeq ORF 2250 bp
Locus ID 20846
Cytogenetics 1 26.81 cM
MW 87.8 kDa
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"NM_009283" in other vectors (3)
SKU Description Size Price
MG227437 Stat1 (tGFP-tagged) - Mouse signal transducer and activator of transcription 1 (Stat1), (10ug) 10 ug
$789.00 $1,229.00
MR227437L3 Lenti ORF clone of Stat1 (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 1 (Stat1), transcript variant 2 10 ug
$1,329.00
MR227437L4 Lenti ORF clone of Stat1 (mGFP-tagged) - Mouse signal transducer and activator of transcription 1 (Stat1), transcript variant 2 10 ug
$1,329.00

file-alt Citations

Zika virus infection triggers caspase cleavage of STAT1
Shu, Ma, Zou et al
Microbiol Spectr (2023)
Cited by 5
AI Summary: OriGene’s Mouse Tagged ORF Clone (MR227437) was used as a source of murine STAT1 cDNA to study the cleavage resistance of mSTAT1 during Zika virus infection in this research.
Snippet: Zika virus (ZIKV) is an important pathogen that causes Guillain-Barre syndrome in adults and congenital Zika syndrome in newborns. Interferon (IFN) triggers intracellular Save;, coding regions of murine STAT1 were amplified from a cDNA clone (Mouse Tagged ORF Clone MR227437, OriGene), flanking the HA tag in the C-termini of t
NS5-independent Ablation of STAT2 by Zika virus to antagonize interferon signalling
Shu, Ma, Zhang et al
Emerg Microbes Infect (2021) 10 (1), 1609-1625 DOI: 10.1080/22221751.2021.1964384
Cited by 14
AI Summary: OriGene’s Mouse Tagged ORF Clones (MR227437 and MR225472) were used as templates to amplify STAT2 coding regions for constructing plasmids to study the role of STAT2 in Zika virus-mediated interferon signaling antagonism.
Snippet: To Generate the plasmids pTRIP-IRES-puro-mSTAT2-HA, coding regions of STAT2 were amplified from the cDNA clones (Mouse Tagged ORF Clone MR227437 and MR225472, OriGene), flanking with the HA tag in the C-termini of the ORFs, and then cloned into the
FigureFigureFigure
Showing 1-2 of 2 papers.
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