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pCMV6-Entry2 Mammalian Expression Vector

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SKU
PS100063
pCMV6-Entry2, mammalian vector with C-terminal Myc-DDK tag, 10ug
  $670.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag Myc-DDK
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • pCMV6-Entry2 vector is the secondary entry vector for TrueORF clones, This vector is used when the insert is difficult to be cloned into the primary entry vector, pCMV6-Entry.
  • pCMV6-Entry2 vector exhibits the same functional properties of pCMV6-Entry, including kanamycin-resistance in E.coli, Neomycin-resistance in mammalian cells, protein expression driven by CMV. The multiple cloning sites are also identical to pCMV6-Entry.
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal Myc-DDK tags. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich).
  • Such clones are the best for detection and purification of the transgene using anti-Myc or anti-DDK antibodies.
  • Serve as the entry vector in the PrecisionShuttle system to transfer the ORF sequence into any destination vectors for other tagging options or other expression platforms.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Ferroptosis is induced by lenvatinib through fibroblast growth factor receptor-4 inhibition in hepatocellular carcinoma
Iseda, Itoh, Toshida et al
Cancer Sci (2022) 113 (7), 2272-2287 DOI: 10.1111/cas.15378
Cited by 113
AI Summary: In this study, OriGene’s Nrf2 plasmid (cat. SC116283, NM_006164) was used to overexpress Nrf2 in hepatocellular carcinoma cells, and OriGene’s empty vector (cat. PS100063, pCMV6‐Entry Vector) served as a control, enabling the authors to assess the role of Nrf2 in lenvatinib-induced ferroptosis.
Snippet: d‐derived 2‐like 2 overexpressing cells and nuclear factor erythroid‐derived 2‐like 2‐ or fibroblast growth factor receptor‐4‐suppressed cells An Nrf2 plasmid (cat. SC116283, NM_006164;Origene) was transfected into HCC cells using a jetPRIME kit (ct. 114, Polyplus Transfection). An empty vector (cat. PS100063, pCMV6‐Entry Vector;Origene) was also transfected into HCC cells as a c
FigureFigureFigure
Showing 1-1 of 1 papers.
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