OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Ephb4 (NM_001159571) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR227085
Ephb4 (Myc-DDK-tagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $912.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Ephb4 or NM_001159571"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ephb4
Synonyms AI042935; Htk; MDK2; Myk1; Tyro11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR227085 representing NM_001159571
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTCCGAGCGCTGCTGTGCTGGGCTTCCCTCGCCACTGCTTTAGAAGAGACCCTGTTGAACACAA
AACTGGAAACGGCGGATCTGAAATGGGTGACTTACCCTCAGGCAGAGGGCCAGTGGGAGGAGCTAAGCGG
CCTGGATGAGGAACAGCACAGCGTCCGCACCTATGAGGTGTGCGACATGAAGCGTCCAGGGGGCCAGGCT
CACTGGCTGCGCACTGGCTGGGTCCCAAGGCGAGGTGCTGTCCACGTGTATGCCACGATACGCTTCACCA
TGATGGAATGCCTGTCCCTGCCGAGGGCCAGTCGCTCCTGCAAGGAGACATTCACTGTCTTCTATTACGA
GAGCGAAGCTGATACGGCCACGGCCCATACGCCCGCCTGGATGGAGAACCCCTACATCAAGGTGGACACA
GTGGCCGCAGAACATCTGACTCGGAAGCGCCCTGGAGCTGAAGCCACAGGGAAAGTTAATATCAAGACGC
TGCGCCTGGGTCCTCTCAGCAAAGCTGGCTTCTACCTGGCTTTCCAGGACCAAGGAGCCTGCATGGCTCT
GCTCTCCCTGCATCTCTTTTACAAGAAGTGCTCCTGGCTGATCACGAACTTGACCTACTTCCCCGAGACG
GTGCCTCGGGAGCTCGTGGTGCCGGTGGCAGGTAGCTGCGTGGCCAACGCGGTCCCTACCGCCAACCCCA
GCCCCAGCCTCTACTGCCGGGAAGATGGTCAATGGGCTGAGCAGCAGGTCACGGGCTGCAGCTGCGCGCC
AGGGTACGAGGCTGCGGAAAGCAACAAAGTATGCAGAGCCTGTGGCCAGGGAACCTTCAAGCCCCAAATA
GGAGACGAGTCCTGCCTGCCATGCCCAGCCAACAGCCACTCGAATAACATTGGGTCTCCTGTCTGCCTGT
GTCGAATTGGGTATTACCGGGCCCGCTCAGACCCCCGGAGTTCACCTTGCACTACCCCACCCTCTGCTCC
AAGAAGCGTGGTTCACCATTTGAATGGTTCCACCCTGCGCCTGGAATGGAGTGCTCCCCTTGAGTCCGGA
GGCCGAGAGGACCTCACTTATGCTGTACGCTGCCGAGAGTGCCGTCCTGGGGGTTCCTGCTTGCCCTGTG
GGGGCGACATGACCTTCGACCCCGGTCCTCGAGACCTGGTTGAGCCCTGGGTGGCAATCCGAGGGCTGCG
TCCTGATGTCACCTATACCTTTGAGGTTGCTGCTTTGAATGGTGTGTCTACCTTAGCCACTGGACCACCT
CCTTTTGAGCCTGTCAATGTCACCACTGACCGTGAGGGGCGCATTGGGTTTCTTTCTCCTGCAGTGCCTC
CTGCAGTGTCTGACATCCGAGTGACTCGGTCGTCACCCAGCAGCTTGATCCTGTCATGGGCTATCCCCAG
AGCACCCAGTGGGGCCGTGCTGGACTACGAGGTCAAGTATCATGAGAAGGGCGCAGAGGGCCCCAGCAGT
GTTCGTTTCCTGAAGACATCAGAAAACCGAGCTGAGCTCCGGGGGCTGAAGCGGGGAGCCAGCTATCTGG
TCCAGGTACGCGCACGGTCCGAGGCTGGCTACGGTCCCTTCGGCCAGGAGCATCACAGTCAGACTCAACT
GGATGAGAGCGAGAGCTGGCGGGAGCAGCTGGCCCTGATTGCAGGCACTGCGGTTGTGGGTGTGGTCCTG
GTCCTGGTGGTCGTCATCATTGCAGTTCTCTGCCTCAGGAAGCAGAGCAATGGGAGGGAAGTTGAGTACT
CGGATAAGCATGGGCAGTATCTCATCGGGCACGGTACCAAGGTCTACATTGATCCTTTTACTTACGAAGA
CCCTAATGAGGCAGTGAGGGAATTTGCCAAAGAGATCGATGTCTCCTATGTCAAGATTGAAGAGGTAATT
GGTGCAGGTGAGTTCGGCGAGGTGTGCCGGGGTCGGCTGAAGGCACCAGGGAAAAAGGAGAGCTGTGTGG
CCATCAAGACTCTGAAGGGTGGCTACACCGAGCGCCAGAGGCGTGAGTTCCTGAGCGAGGCCTCCATCAT
GGGCCAGTTCGAGCATCCCAACATCATCCGCCTCGAGGGCGTGGTCACCAACAGTGTGCCGGTTATGATC
CTCACGGAATTCATGGAGAACGGAGCCCTGGACTCCTTCCTGCGGCTGAACGACGGGCAGTTCACAGTCA
TCCAGCTGGTGGGCATGCTGAGGGGCATCGCCTCGGGCATGCGGTACCTGGCTGAAATGAGCTATGTCCA
CCGAGACCTGGCTGCTCGGAACATCTTGGTCAACAGTAACCTGGTCTGCAAGGTGTCCGACTTTGGCCTC
TCCAGATTCTTGGAGGAGAACTCCTCTGATCCCACCTACACAAGTTCCCTGGGAGGCAAGATTCCCATCC
GATGGACCGCCCCTGAAGCCATTGCCTTCAGGAAGTTCACCTCTGCCAGTGATGCCTGGAGCTATGGGAT
CGTCATGTGGGAGGTCATGTCTTTTGGGGAACGGCCATACTGGGACATGAGCAACCAGGATGTGATCAAT
GCCATTGAACAGGACTACCGGCTGCCTCCTCCTCCAGACTGCCCCACCTCCCTCCACCAGCTCATGCTGG
ATTGTTGGCAGAAGGACCGGAATGCCCGGCCCCGCTTTCCCCAGGTGGTCAGCGCTCTGGACAAGATGAT
CCGGAATCCCGCTAGCCTCAAAATCGTGGCCAGGGAGAATGGCGGGGCCTCACATCCACTCTTGGACCAA
CGGCAGCCTCACTATTCTGCTTTCGGTTCTGTGGGCGAGTGGCTTCGAGCCATCAAGATGGGAAGATACG
AGGAAAGTTTTGCAGCGGCTGGATTCGGCTCCTTTGAGGTGGTCAGTCAGATCTCTGCCGAGGACCTTCT
CCGAATTGGAGTCACTCTGGCAGGACACCAGAAGAAAATCTTGGCCAGTGTGCAGCATATGAAGTCCCAA
GCTAAGCCAGGAGCCCCTGGTGGGACAGGGGGACCAGCCCAGCAGTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR227085 representing NM_001159571
Red=Cloning site Green=Tags(s)

MELRALLCWASLATALEETLLNTKLETADLKWVTYPQAEGQWEELSGLDEEQHSVRTYEVCDMKRPGGQA
HWLRTGWVPRRGAVHVYATIRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDT
VAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCSWLITNLTYFPET
VPRELVVPVAGSCVANAVPTANPSPSLYCREDGQWAEQQVTGCSCAPGYEAAESNKVCRACGQGTFKPQI
GDESCLPCPANSHSNNIGSPVCLCRIGYYRARSDPRSSPCTTPPSAPRSVVHHLNGSTLRLEWSAPLESG
GREDLTYAVRCRECRPGGSCLPCGGDMTFDPGPRDLVEPWVAIRGLRPDVTYTFEVAALNGVSTLATGPP
PFEPVNVTTDREGRIGFLSPAVPPAVSDIRVTRSSPSSLILSWAIPRAPSGAVLDYEVKYHEKGAEGPSS
VRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESESWREQLALIAGTAVVGVVL
VLVVVIIAVLCLRKQSNGREVEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVI
GAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSVPVMI
LTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGL
SRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVIN
AIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQ
RQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFEVVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQ
AKPGAPGGTGGPAQQF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001159571
ORF Size 2988 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001159571.1, NP_001153043.1
RefSeq Size 4361 bp
RefSeq ORF 2991 bp
Locus ID 13846
Cytogenetics 5 G2
MW 110.2 kDa
Summary Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_001159571" in other vectors (4)
SKU Description Size Price
MC223038 Ephb4 (untagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 1, (10ug) 10 ug
$1,004.00
MG227085 Ephb4 (tGFP-tagged) - Mouse Eph receptor B4 (Ephb4) transcript variant 1, (10ug) 10 ug
$789.00 $1,112.00
MR227085L3 Lenti ORF clone of Ephb4 (Myc-DDK-tagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 1 10 ug
$1,212.00
MR227085L4 Lenti ORF clone of Ephb4 (mGFP-tagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 1 10 ug
$1,212.00

Plasmid Map for MR227085

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR227085
Circular map for MR227085

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products