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Ephb4 (NM_001159571) Mouse Untagged Clone

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SKU
MC223038
Ephb4 (untagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 1, (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $1,004.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ephb4
Synonyms AI042935; Htk; MDK2; Myk1; Tyro11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC223038 representing NM_001159571
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTCCGAGCGCTGCTGTGCTGGGCTTCCCTCGCCACTGCTTTAGAAGAGACCCTGTTGAACACAA
AACTGGAAACGGCGGATCTGAAATGGGTGACTTACCCTCAGGCAGAGGGCCAGTGGGAGGAGCTAAGCGG
CCTGGATGAGGAACAGCACAGCGTCCGCACCTATGAGGTGTGCGACATGAAGCGTCCAGGGGGCCAGGCT
CACTGGCTGCGCACTGGCTGGGTCCCAAGGCGAGGTGCTGTCCACGTGTATGCCACGATACGCTTCACCA
TGATGGAATGCCTGTCCCTGCCGAGGGCCAGTCGCTCCTGCAAGGAGACATTCACTGTCTTCTATTACGA
GAGCGAAGCTGATACGGCCACGGCCCATACGCCCGCCTGGATGGAGAACCCCTACATCAAGGTGGACACA
GTGGCCGCAGAACATCTGACTCGGAAGCGCCCTGGAGCTGAAGCCACAGGGAAAGTTAATATCAAGACGC
TGCGCCTGGGTCCTCTCAGCAAAGCTGGCTTCTACCTGGCTTTCCAGGACCAAGGAGCCTGCATGGCTCT
GCTCTCCCTGCATCTCTTTTACAAGAAGTGCTCCTGGCTGATCACGAACTTGACCTACTTCCCCGAGACG
GTGCCTCGGGAGCTCGTGGTGCCGGTGGCAGGTAGCTGCGTGGCCAACGCGGTCCCTACCGCCAACCCCA
GCCCCAGCCTCTACTGCCGGGAAGATGGTCAATGGGCTGAGCAGCAGGTCACGGGCTGCAGCTGCGCGCC
AGGGTACGAGGCTGCGGAAAGCAACAAAGTATGCAGAGCCTGTGGCCAGGGAACCTTCAAGCCCCAAATA
GGAGACGAGTCCTGCCTGCCATGCCCAGCCAACAGCCACTCGAATAACATTGGGTCTCCTGTCTGCCTGT
GTCGAATTGGGTATTACCGGGCCCGCTCAGACCCCCGGAGTTCACCTTGCACTACCCCACCCTCTGCTCC
AAGAAGCGTGGTTCACCATTTGAATGGTTCCACCCTGCGCCTGGAATGGAGTGCTCCCCTTGAGTCCGGA
GGCCGAGAGGACCTCACTTATGCTGTACGCTGCCGAGAGTGCCGTCCTGGGGGTTCCTGCTTGCCCTGTG
GGGGCGACATGACCTTCGACCCCGGTCCTCGAGACCTGGTTGAGCCCTGGGTGGCAATCCGAGGGCTGCG
TCCTGATGTCACCTATACCTTTGAGGTTGCTGCTTTGAATGGTGTGTCTACCTTAGCCACTGGACCACCT
CCTTTTGAGCCTGTCAATGTCACCACTGACCGTGAGGGGCGCATTGGGTTTCTTTCTCCTGCAGTGCCTC
CTGCAGTGTCTGACATCCGAGTGACTCGGTCGTCACCCAGCAGCTTGATCCTGTCATGGGCTATCCCCAG
AGCACCCAGTGGGGCCGTGCTGGACTACGAGGTCAAGTATCATGAGAAGGGCGCAGAGGGCCCCAGCAGT
GTTCGTTTCCTGAAGACATCAGAAAACCGAGCTGAGCTCCGGGGGCTGAAGCGGGGAGCCAGCTATCTGG
TCCAGGTACGCGCACGGTCCGAGGCTGGCTACGGTCCCTTCGGCCAGGAGCATCACAGTCAGACTCAACT
GGATGAGAGCGAGAGCTGGCGGGAGCAGCTGGCCCTGATTGCAGGCACTGCGGTTGTGGGTGTGGTCCTG
GTCCTGGTGGTCGTCATCATTGCAGTTCTCTGCCTCAGGAAGCAGAGCAATGGGAGGGAAGTTGAGTACT
CGGATAAGCATGGGCAGTATCTCATCGGGCACGGTACCAAGGTCTACATTGATCCTTTTACTTACGAAGA
CCCTAATGAGGCAGTGAGGGAATTTGCCAAAGAGATCGATGTCTCCTATGTCAAGATTGAAGAGGTAATT
GGTGCAGGTGAGTTCGGCGAGGTGTGCCGGGGTCGGCTGAAGGCACCAGGGAAAAAGGAGAGCTGTGTGG
CCATCAAGACTCTGAAGGGTGGCTACACCGAGCGCCAGAGGCGTGAGTTCCTGAGCGAGGCCTCCATCAT
GGGCCAGTTCGAGCATCCCAACATCATCCGCCTCGAGGGCGTGGTCACCAACAGTGTGCCGGTTATGATC
CTCACGGAATTCATGGAGAACGGAGCCCTGGACTCCTTCCTGCGGCTGAACGACGGGCAGTTCACAGTCA
TCCAGCTGGTGGGCATGCTGAGGGGCATCGCCTCGGGCATGCGGTACCTGGCTGAAATGAGCTATGTCCA
CCGAGACCTGGCTGCTCGGAACATCTTGGTCAACAGTAACCTGGTCTGCAAGGTGTCCGACTTTGGCCTC
TCCAGATTCTTGGAGGAGAACTCCTCTGATCCCACCTACACAAGTTCCCTGGGAGGCAAGATTCCCATCC
GATGGACCGCCCCTGAAGCCATTGCCTTCAGGAAGTTCACCTCTGCCAGTGATGCCTGGAGCTATGGGAT
CGTCATGTGGGAGGTCATGTCTTTTGGGGAACGGCCATACTGGGACATGAGCAACCAGGATGTGATCAAT
GCCATTGAACAGGACTACCGGCTGCCTCCTCCTCCAGACTGCCCCACCTCCCTCCACCAGCTCATGCTGG
ATTGTTGGCAGAAGGACCGGAATGCCCGGCCCCGCTTTCCCCAGGTGGTCAGCGCTCTGGACAAGATGAT
CCGGAATCCCGCTAGCCTCAAAATCGTGGCCAGGGAGAATGGCGGGGCCTCACATCCACTCTTGGACCAA
CGGCAGCCTCACTATTCTGCTTTCGGTTCTGTGGGCGAGTGGCTTCGAGCCATCAAGATGGGAAGATACG
AGGAAAGTTTTGCAGCGGCTGGATTCGGCTCCTTTGAGGTGGTCAGTCAGATCTCTGCCGAGGACCTTCT
CCGAATTGGAGTCACTCTGGCAGGACACCAGAAGAAAATCTTGGCCAGTGTGCAGCATATGAAGTCCCAA
GCTAAGCCAGGAGCCCCTGGTGGGACAGGGGGACCAGCCCAGCAGTTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001159571
Insert Size 2991 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001159571.1, NP_001153043.1
RefSeq Size 4361 bp
RefSeq ORF 2991 bp
Locus ID 13846
Cytogenetics 5 G2
Summary Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).
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Documents
Resources
"NM_001159571" in other vectors (4)
SKU Description Size Price
MG227085 Ephb4 (tGFP-tagged) - Mouse Eph receptor B4 (Ephb4) transcript variant 1, (10ug) 10 ug
$789.00 $1,112.00
MR227085 Ephb4 (Myc-DDK-tagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 1 10 ug
$589.00 $912.00
MR227085L3 Lenti ORF clone of Ephb4 (Myc-DDK-tagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 1 10 ug
$1,212.00
MR227085L4 Lenti ORF clone of Ephb4 (mGFP-tagged) - Mouse Eph receptor B4 (Ephb4), transcript variant 1 10 ug
$1,212.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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