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Ppargc1b (NM_133249) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR226760
Ppargc1b (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $884.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppargc1b
Synonyms 4631412G21Rik; Perc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226760 representing NM_133249
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATT
CGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC

ATGGCGGGGAACGACTGCGGCGCGCTGCTGGATGAAGAGCTCTCGTCCTTCTTCCTCAAC
TATCTCTCTGACACGCAGGGTGGGGACTCTGGAGAGGAACAGCTGTGTGCTGACTTGCCA
GAGCTTGACCTCTCCCAGCTGGACGCCAGTGACTTTGACTCAGCCACGTGCTTTGGGGAG
CTGCAGTGGTGCCCGGAGACCTCAGAGACAGAGCCCAGCCAGTACAGCCCCGATGACTCC
GAGCTCTTCCAGATTGACAGTGAGAATGAAGCTCTCTTGGCTGCGCTTACGAAGACCCTG
GATGACATCCCCGAAGACGATGTGGGGCTGGCTGCCTTCCCAGAACTGGATGAAGGCGAC
ACACCATCCTGCACCCCAGCCTCACCTGCCCCCTTATCTGCACCCCCCAGCCCCACCCTG
GAGAGGCTTCTGTCCCCAGCGTCTGACGTGGACGAGCTTTCACTGCTACAGAAGCTCCTC
CTGGCCACATCCTCCCCAACAGCAAGCTCTGACGCTCTGAAGGACGGGGCCACCTGGTCC
CAGACCAGCCTCAGTTCCAGAAGTCAGCGGCCTTGTGTCAAGGTGGATGGCACCCAGGAT
AAGAAGACCCCCACACTGCGGGCTCAGAGCCGGCCTTGTACGGAACTGCATAAGCACCTC
ACTTCGGTGCTGCCCTGTCCCAGAGTGAAAGCCTGCTCCCCAGCTCCGCACCCGAGCCCT
CGGCTCCTCTCCAAAGAGGAGGAGGAGGAGGTGGGGGAGGATTGCCCAAGCCCTTGGCCG
ACTCCAGCCTCGCCCCAAGACTCCCTAGCACAGGACACGGCCAGCCCCGACAGTGCCCAG
GCTCCCGAGGAGGATGTGAGGGCCATGGTACAGCTCATTCGCTACATGCATACCTACTGC
CTGCCTCAGAGGAAGCTGCCCCAACGGGCCCCAGAGCCAATCCCCCAGGCCTGCAGCAGC
CTCTCCAGGCAGGTTCAACCCCGATCCCGGCATCCCCCCAAAGCCTTCTGGACTGAGTTC
TCTATCCTAAGGGAACTTCTGGCCCAAGATATCCTCTGTGATGTTAGCAAGCCCTACCGC
CTGGCCATACCTGTCTATGCTTCCCTCACACCTCAGTCCAGGCCCAGGCCCCCCAAGGAC
AGTCAGGCCTCCCCTGCCTACTCTGCCATGGCAGAAGAGGTGAGAATCACTGCTTCCCCC
AAGAGCACCGGGCCTAGACCCAGCCTGCGTCCTCTGAGGCTGGAGGTGAAACGGGATGTT
AACAAGCCTACAAGGCAAAAGCGGGAGGAAGATGAGGAGGAGGAGGAGGAAGAAGAAGAA
GAGGAAGAAGAAAAAGAAGAGGAAGAAGAGGAGTGGGGCAGGAAGAGACCAGGTCGTGGC
CTGCCATGGACCAAACTAGGGAGGAAGATGGACAGCTCCGTGTGCCCCGTGCGGCGCTCC
AGGAGACTGAATCCAGAGCTGGGTCCCTGGCTGACATTCACTGATGAGCCCTTAGGTGCT
CTGCCCTCGATGTGCCTGGATACAGAGACCCACAACCTGGAGGAAGACCTGGGCAGCCTC
ACAGACAGTAGTCAAGGCCGGCAGCTCCCCCAGGGATCCCAGATCCCCGCCCTGGAAAGC
CCCTGTGAGAGTGGGTGCGGAGACACAGATGAAGATCCAAGCTGCCCACAGCCCACTTCC
AGAGACTCCTCCAGGTGCCTCATGCTGGCCTTGTCACAAAGCGACTCTCTTGGCAAGAAG
AGCTTTGAGGAGTCCCTGACGGTGGAGCTTTGCGGCACGGCAGGACTCACGCCACCCACC
ACACCTCCATACAAGCCAATGGAGGAGGACCCCTTCAAGCCAGACACCAAGCTCAGCCCA
GGCCAAGACACAGCTCCCAGCCTTCCCTCCCCCGAGGCTCTTCCGCTCACAGCCACCCCA
GGAGCTTCCCACAAGCTGCCCAAGAGGCACCCAGAGCGAAGCGAGCTCCTGTCCCATTTG
CAGCATGCCACAACCCAACCAGTCTCACAGGCTGGCCAGAAGCGCCCCTTCTCCTGCTCC
TTTGGAGACCACGACTACTGCCAGGTGCTCAGGCCAGAGGCTGCCCTGCAGAGGAAGGTG
CTGCGGTCCTGGGAGCCAATCGGGGTCCACCTTGAAGACTTGGCCCAGCAGGGTGCCCCT
CTGCCAACGGAAACAAAGGCCCCTAGGAGGGAGGCAAACCAGAACTGTGACCCTACCCAC
AAGGACAGCATGCAGCTAAGAGACCATGAGATCCGTGCCAGTCTCACAAAGCACTTTGGG
CTGCTGGAGACTGCTCTGGAAGGTGAAGACCTGGCGTCCTGTAAAAGCCCGGAGTATGAC
ACCGTATTTGAGGACAGCAGCAGCAGCAGTGGCGAGAGTAGCTTCCTGCTTGAGGAGGAG
GAGGAAGAGGAGGAGGGAGGGGAAGAGGACGATGAAGGAGAGGACTCAGGGGTCAGCCCT
CCCTGCTCTGATCACTGCCCCTACCAGAGCCCACCCAGTAAGGCCAGTCGGCAGCTCTGC
TCCCGAAGCCGCTCCAGTTCCGGCTCCTCGTCCTGCAGCTCCTGGTCACCAGCCACCCGG
AAGAACTTCAGACGTGAGAGCAGAGGGCCCTGTTCAGATGGAACCCCAAGCGTCCGGCAT
GCCAGGAAGCGGCGGGAAAAGGCCATCGGTGAAGGCCGTGTGGTATACATTCGAAATCTC
TCCAGTGACATGAGCTCTCGGGAACTAAAGAAGCGCTTTGAGGTGTTCGGTGAGATTGTA
GAGTGCCAGGTGCTGACGAGAAGTAAAAGAGGCCAGAAGCACGGTTTTATCACCTTCCGG
TGTTCAGAGCACGCTGCCCTGTCCGTGAGGAACGGCGCCACCCTGAGAAAGCGCAATGAG
CCCTCCTTCCACCTGAGCTATGGAGGGCTCCGGCACTTCCGTTGGCCCAGATACACTGAC
TATGATCCCACATCTGAGGAGTCCCTTCCCTCATCTGGGAAAAGCAAGTACGAAGCCATG
GATTTTGACAGCTTACTGAAAGAGGCCCAGCAGAGCCTGCAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGAT
GACGACGATAAGGTTTAAA
Protein Sequence
>MR226760 protein sequence
Blue=ORF Red=Cloning site Green=Tag(s)

MAGNDCGALLDEELSSFFLNYLSDTQGGDSGEEQLCADLPELDLSQLDASDFDSATCFGE
LQWCPETSETEPSQYSPDDSELFQIDSENEALLAALTKTLDDIPEDDVGLAAFPELDEGD
TPSCTPASPAPLSAPPSPTLERLLSPASDVDELSLLQKLLLATSSPTASSDALKDGATWS
QTSLSSRSQRPCVKVDGTQDKKTPTLRAQSRPCTELHKHLTSVLPCPRVKACSPAPHPSP
RLLSKEEEEEVGEDCPSPWPTPASPQDSLAQDTASPDSAQAPEEDVRAMVQLIRYMHTYC
LPQRKLPQRAPEPIPQACSSLSRQVQPRSRHPPKAFWTEFSILRELLAQDILCDVSKPYR
LAIPVYASLTPQSRPRPPKDSQASPAYSAMAEEVRITASPKSTGPRPSLRPLRLEVKRDV
NKPTRQKREEDEEEEEEEEEEEEEKEEEEEEWGRKRPGRGLPWTKLGRKMDSSVCPVRRS
RRLNPELGPWLTFTDEPLGALPSMCLDTETHNLEEDLGSLTDSSQGRQLPQGSQIPALES
PCESGCGDTDEDPSCPQPTSRDSSRCLMLALSQSDSLGKKSFEESLTVELCGTAGLTPPT
TPPYKPMEEDPFKPDTKLSPGQDTAPSLPSPEALPLTATPGASHKLPKRHPERSELLSHL
QHATTQPVSQAGQKRPFSCSFGDHDYCQVLRPEAALQRKVLRSWEPIGVHLEDLAQQGAP
LPTETKAPRREANQNCDPTHKDSMQLRDHEIRASLTKHFGLLETALEGEDLASCKSPEYD
TVFEDSSSSSGESSFLLEEEEEEEEGGEEDDEGEDSGVSPPCSDHCPYQSPPSKASRQLC
SRSRSSSGSSSCSSWSPATRKNFRRESRGPCSDGTPSVRHARKRREKAIGEGRVVYIRNL
SSDMSSRELKKRFEVFGEIVECQVLTRSKRGQKHGFITFRCSEHAALSVRNGATLRKRNE
PSFHLSYGGLRHFRWPRYTDYDPTSEESLPSSGKSKYEAMDFDSLLKEAQQSLH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133249
ORF Size 3042 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133249.1, NM_133249.2, NP_573512.1
RefSeq Size 3656 bp
RefSeq ORF 3045 bp
Locus ID 170826
UniProt ID Q8VHJ7
Cytogenetics 18 E1
MW 112 kDa
Summary Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcriptional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be part of the pathways regulating the elevation of gluconeogenesis, beta-oxidation of fatty acids and ketogenesis during fasting. Stimulates SREBP-mediated lipogenic gene expression in the liver. Induces energy expenditure and antagonizes obesity when overexpressed. Induces also the expression of mitochondrial genes involved in oxidative metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133249" in other vectors (4)
SKU Description Size Price
MC223214 Ppargc1b (untagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b), (10ug) 10 ug
$972.00
MG226760 Ppargc1b (tGFP-tagged) - Mouse peroxisome proliferative activated receptor gamma coactivator 1 beta (Ppargc1b), (10ug) 10 ug
$1,084.00
MR226760L3 Lenti ORF clone of Ppargc1b (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b) 10 ug
$1,184.00
MR226760L4 Lenti ORF clone of Ppargc1b (mGFP-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b) 10 ug
$1,184.00

Plasmid Map for MR226760

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226760
Circular map for MR226760

file-alt Citations

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