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Ppargc1b (NM_133249) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG226760
Ppargc1b (tGFP-tagged) - Mouse peroxisome proliferative activated receptor gamma coactivator 1 beta (Ppargc1b), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,084.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppargc1b
Synonyms 4631412G21Rik; Perc
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG226760 representing NM_133249
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGGAACGACTGCGGCGCGCTGCTGGATGAAGAGCTCTCGTCCTTCTTCCTCAACTATCTCTCTG
ACACGCAGGGTGGGGACTCTGGAGAGGAACAGCTGTGTGCTGACTTGCCAGAGCTTGACCTCTCCCAGCT
GGACGCCAGTGACTTTGACTCAGCCACGTGCTTTGGGGAGCTGCAGTGGTGCCCGGAGACCTCAGAGACA
GAGCCCAGCCAGTACAGCCCCGATGACTCCGAGCTCTTCCAGATTGACAGTGAGAATGAAGCTCTCTTGG
CTGCGCTTACGAAGACCCTGGATGACATCCCCGAAGACGATGTGGGGCTGGCTGCCTTCCCAGAACTGGA
TGAAGGCGACACACCATCCTGCACCCCAGCCTCACCTGCCCCCTTATCTGCACCCCCCAGCCCCACCCTG
GAGAGGCTTCTGTCCCCAGCGTCTGACGTGGACGAGCTTTCACTGCTACAGAAGCTCCTCCTGGCCACAT
CCTCCCCAACAGCAAGCTCTGACGCTCTGAAGGACGGGGCCACCTGGTCCCAGACCAGCCTCAGTTCCAG
AAGTCAGCGGCCTTGTGTCAAGGTGGATGGCACCCAGGATAAGAAGACCCCCACACTGCGGGCTCAGAGC
CGGCCTTGTACGGAACTGCATAAGCACCTCACTTCGGTGCTGCCCTGTCCCAGAGTGAAAGCCTGCTCCC
CAGCTCCGCACCCGAGCCCTCGGCTCCTCTCCAAAGAGGAGGAGGAGGAGGTGGGGGAGGATTGCCCAAG
CCCTTGGCCGACTCCAGCCTCGCCCCAAGACTCCCTAGCACAGGACACGGCCAGCCCCGACAGTGCCCAG
GCTCCCGAGGAGGATGTGAGGGCCATGGTACAGCTCATTCGCTACATGCATACCTACTGCCTGCCTCAGA
GGAAGCTGCCCCAACGGGCCCCAGAGCCAATCCCCCAGGCCTGCAGCAGCCTCTCCAGGCAGGTTCAACC
CCGATCCCGGCATCCCCCCAAAGCCTTCTGGACTGAGTTCTCTATCCTAAGGGAACTTCTGGCCCAAGAT
ATCCTCTGTGATGTTAGCAAGCCCTACCGCCTGGCCATACCTGTCTATGCTTCCCTCACACCTCAGTCCA
GGCCCAGGCCCCCCAAGGACAGTCAGGCCTCCCCTGCCTACTCTGCCATGGCAGAAGAGGTGAGAATCAC
TGCTTCCCCCAAGAGCACCGGGCCTAGACCCAGCCTGCGTCCTCTGAGGCTGGAGGTGAAACGGGATGTT
AACAAGCCTACAAGGCAAAAGCGGGAGGAAGATGAGGAGGAGGAGGAGGAAGAAGAAGAAGAGGAAGAAG
AAAAAGAAGAGGAAGAAGAGGAGTGGGGCAGGAAGAGACCAGGTCGTGGCCTGCCATGGACCAAACTAGG
GAGGAAGATGGACAGCTCCGTGTGCCCCGTGCGGCGCTCCAGGAGACTGAATCCAGAGCTGGGTCCCTGG
CTGACATTCACTGATGAGCCCTTAGGTGCTCTGCCCTCGATGTGCCTGGATACAGAGACCCACAACCTGG
AGGAAGACCTGGGCAGCCTCACAGACAGTAGTCAAGGCCGGCAGCTCCCCCAGGGATCCCAGATCCCCGC
CCTGGAAAGCCCCTGTGAGAGTGGGTGCGGAGACACAGATGAAGATCCAAGCTGCCCACAGCCCACTTCC
AGAGACTCCTCCAGGTGCCTCATGCTGGCCTTGTCACAAAGCGACTCTCTTGGCAAGAAGAGCTTTGAGG
AGTCCCTGACGGTGGAGCTTTGCGGCACGGCAGGACTCACGCCACCCACCACACCTCCATACAAGCCAAT
GGAGGAGGACCCCTTCAAGCCAGACACCAAGCTCAGCCCAGGCCAAGACACAGCTCCCAGCCTTCCCTCC
CCCGAGGCTCTTCCGCTCACAGCCACCCCAGGAGCTTCCCACAAGCTGCCCAAGAGGCACCCAGAGCGAA
GCGAGCTCCTGTCCCATTTGCAGCATGCCACAACCCAACCAGTCTCACAGGCTGGCCAGAAGCGCCCCTT
CTCCTGCTCCTTTGGAGACCACGACTACTGCCAGGTGCTCAGGCCAGAGGCTGCCCTGCAGAGGAAGGTG
CTGCGGTCCTGGGAGCCAATCGGGGTCCACCTTGAAGACTTGGCCCAGCAGGGTGCCCCTCTGCCAACGG
AAACAAAGGCCCCTAGGAGGGAGGCAAACCAGAACTGTGACCCTACCCACAAGGACAGCATGCAGCTAAG
AGACCATGAGATCCGTGCCAGTCTCACAAAGCACTTTGGGCTGCTGGAGACTGCTCTGGAAGGTGAAGAC
CTGGCGTCCTGTAAAAGCCCGGAGTATGACACCGTATTTGAGGACAGCAGCAGCAGCAGTGGCGAGAGTA
GCTTCCTGCTTGAGGAGGAGGAGGAAGAGGAGGAGGGAGGGGAAGAGGACGATGAAGGAGAGGACTCAGG
GGTCAGCCCTCCCTGCTCTGATCACTGCCCCTACCAGAGCCCACCCAGTAAGGCCAGTCGGCAGCTCTGC
TCCCGAAGCCGCTCCAGTTCCGGCTCCTCGTCCTGCAGCTCCTGGTCACCAGCCACCCGGAAGAACTTCA
GACGTGAGAGCAGAGGGCCCTGTTCAGATGGAACCCCAAGCGTCCGGCATGCCAGGAAGCGGCGGGAAAA
GGCCATCGGTGAAGGCCGTGTGGTATACATTCGAAATCTCTCCAGTGACATGAGCTCTCGGGAACTAAAG
AAGCGCTTTGAGGTGTTCGGTGAGATTGTAGAGTGCCAGGTGCTGACGAGAAGTAAAAGAGGCCAGAAGC
ACGGTTTTATCACCTTCCGGTGTTCAGAGCACGCTGCCCTGTCCGTGAGGAACGGCGCCACCCTGAGAAA
GCGCAATGAGCCCTCCTTCCACCTGAGCTATGGAGGGCTCCGGCACTTCCGTTGGCCCAGATACACTGAC
TATGATCCCACATCTGAGGAGTCCCTTCCCTCATCTGGGAAAAGCAAGTACGAAGCCATGGATTTTGACA
GCTTACTGAAAGAGGCCCAGCAGAGCCTGCAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG226760 representing NM_133249
Red=Cloning site Green=Tags(s)

MAGNDCGALLDEELSSFFLNYLSDTQGGDSGEEQLCADLPELDLSQLDASDFDSATCFGELQWCPETSET
EPSQYSPDDSELFQIDSENEALLAALTKTLDDIPEDDVGLAAFPELDEGDTPSCTPASPAPLSAPPSPTL
ERLLSPASDVDELSLLQKLLLATSSPTASSDALKDGATWSQTSLSSRSQRPCVKVDGTQDKKTPTLRAQS
RPCTELHKHLTSVLPCPRVKACSPAPHPSPRLLSKEEEEEVGEDCPSPWPTPASPQDSLAQDTASPDSAQ
APEEDVRAMVQLIRYMHTYCLPQRKLPQRAPEPIPQACSSLSRQVQPRSRHPPKAFWTEFSILRELLAQD
ILCDVSKPYRLAIPVYASLTPQSRPRPPKDSQASPAYSAMAEEVRITASPKSTGPRPSLRPLRLEVKRDV
NKPTRQKREEDEEEEEEEEEEEEEKEEEEEEWGRKRPGRGLPWTKLGRKMDSSVCPVRRSRRLNPELGPW
LTFTDEPLGALPSMCLDTETHNLEEDLGSLTDSSQGRQLPQGSQIPALESPCESGCGDTDEDPSCPQPTS
RDSSRCLMLALSQSDSLGKKSFEESLTVELCGTAGLTPPTTPPYKPMEEDPFKPDTKLSPGQDTAPSLPS
PEALPLTATPGASHKLPKRHPERSELLSHLQHATTQPVSQAGQKRPFSCSFGDHDYCQVLRPEAALQRKV
LRSWEPIGVHLEDLAQQGAPLPTETKAPRREANQNCDPTHKDSMQLRDHEIRASLTKHFGLLETALEGED
LASCKSPEYDTVFEDSSSSSGESSFLLEEEEEEEEGGEEDDEGEDSGVSPPCSDHCPYQSPPSKASRQLC
SRSRSSSGSSSCSSWSPATRKNFRRESRGPCSDGTPSVRHARKRREKAIGEGRVVYIRNLSSDMSSRELK
KRFEVFGEIVECQVLTRSKRGQKHGFITFRCSEHAALSVRNGATLRKRNEPSFHLSYGGLRHFRWPRYTD
YDPTSEESLPSSGKSKYEAMDFDSLLKEAQQSLH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133249
ORF Size 3042 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133249.2, NP_573512.1
RefSeq Size 3656 bp
RefSeq ORF 3045 bp
Locus ID 170826
UniProt ID Q8VHJ7
Cytogenetics 18 E1
Summary Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcriptional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be part of the pathways regulating the elevation of gluconeogenesis, beta-oxidation of fatty acids and ketogenesis during fasting. Stimulates SREBP-mediated lipogenic gene expression in the liver. Induces energy expenditure and antagonizes obesity when overexpressed. Induces also the expression of mitochondrial genes involved in oxidative metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133249" in other vectors (4)
SKU Description Size Price
MC223214 Ppargc1b (untagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b), (10ug) 10 ug
$972.00
MR226760 Ppargc1b (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b) 10 ug
$589.00 $884.00
MR226760L3 Lenti ORF clone of Ppargc1b (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b) 10 ug
$1,184.00
MR226760L4 Lenti ORF clone of Ppargc1b (mGFP-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 beta (Ppargc1b) 10 ug
$1,184.00

Plasmid Map for MG226760

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG226760
Circular map for MG226760

file-alt Citations

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