Hdac2 (NM_008229) Mouse Tagged ORF Clone
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Hdac2 |
| Synonyms | D10Wsu179e; mRPD3; YAF1; Yy1bp |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008229 |
| ORF Size | 1464 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008229.2, NP_032255.2 |
| RefSeq Size | 2004 bp |
| RefSeq ORF | 1467 bp |
| Locus ID | 15182 |
| UniProt ID | P70288 |
| Cytogenetics | 10 B1 |
| MW | 55.8 kDa |
| Summary | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008229" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC216798 | Hdac2 (untagged) - Mouse histone deacetylase 2 (Hdac2), (10ug) | 10 ug |
$732.00
|
|
| MG226709 | Hdac2 (tGFP-tagged) - Mouse histone deacetylase 2 (Hdac2), (10ug) | 10 ug |
$886.00
|
|
| MR226709L1 | Lenti ORF clone of Hdac2 (Myc-DDK-tagged) - Mouse histone deacetylase 2 (Hdac2) | 10 ug |
$986.00
|
|
| MR226709L2 | Lenti ORF clone of Hdac2 (mGFP-tagged) - Mouse histone deacetylase 2 (Hdac2) | 10 ug |
$986.00
|
|
| MR226709L3 | Lenti ORF clone of Hdac2 (Myc-DDK-tagged) - Mouse histone deacetylase 2 (Hdac2) | 10 ug |
$986.00
|
|
| MR226709L4 | Lenti ORF clone of Hdac2 (mGFP-tagged) - Mouse histone deacetylase 2 (Hdac2) | 10 ug |
$986.00
|
|
Citations
Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung
Lüdtke, Wojahn, Kleppa et al
Respir Res (2021) 22 (1), 85 DOI: 10.1186/s12931-021-01679-y
Lüdtke, Wojahn, Kleppa et al
Respir Res (2021) 22 (1), 85 DOI: 10.1186/s12931-021-01679-y
Cited by 19
AI Summary: OriGene’s pCMV6-Entry.Cbx3-Myc-DDK plasmid (#MR224357) was used in this study to express full-length mouse CBX3, enabling the identification and validation of CBX3 as a TBX2-interacting protein in the developing lung.
Snippet: Heike Pöpperl, Institute for Biophysical Chemistry, Hannover Medical School, Germany);pd2EGFP-N1 (EGFP-expression vector) [35];pCMV6-Entry.Cbx3-Myc-DDK encoding full-length mouse CBX3 (#MR224357, OriGene, Rockville, Maryland, USA);pCMV-SPORT6.Hdac1 encoding full-length mouse HDAC1 (#4217199, Sourcebioscience, Nottingham, UK);pCMV6-Entry.Hdac2-Myc-DDK encoding full-length mouse HDAC2 (#MR2267
Acetylation is essential for nuclear heme oxygenase-1-enhanced tumor growth and invasiveness
Hsu, Chiang, Li et al
Oncogene (2017) 36 (49), 6805-6814 DOI: 10.1038/onc.2017.294
Hsu, Chiang, Li et al
Oncogene (2017) 36 (49), 6805-6814 DOI: 10.1038/onc.2017.294
Cited by 38
AI Summary: In this study, OriGene products including the Myc-DDK-tagged HO-1 expression plasmid (RC210307) and HO-1-specific antibody (TA500429) were used to overexpress and detect HO-1 protein, respectively, enabling the investigation of HO-1 acetylation and its role in cancer cell growth and invasiveness.
Showing 1-2 of 2 papers.
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