Epha2 (NM_010139) Mouse Tagged ORF Clone

SKU MR226151

Epha2 (Myc-DDK-tagged) - Mouse Eph receptor A2 (Epha2)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Epha2
• Synonyms: AW545284; Eck; Myk2; Sek-2; Sek2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226151 representing NM_010139; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGCTCCGGGCAGTCGGTTTCTGCCTGGCGCTGCTGTGGGGTTGCGCGCTGGCGGCCGCGGCGGCAC; AGGGAAAGGAAGTTGTTTTGTTGGACTTCGCAGCAATGAAGGGAGAGCTCGGCTGGCTCACGCACCCCTA; TGGCAAAGGGTGGGACCTGATGCAGAACATCATGGACGACATGCCTATCTACATGTACTCGGTGTGCAAC; GTGGTATCCGGCGACCAGGACAACTGGCTCCGCACCAACTGGGTGTACCGGGAGGAGGCCGAGCGCATCT; TTATTGAGCTCAAGTTCACGGTGCGAGACTGTAACAGCTTCCCGGGTGGCGCCAGCTCATGCAAAGAGAC; CTTCAACCTCTACTATGCAGAGTCAGATGTGGACTATGGCACCAACTTCCAGAAGCGCCAGTTCACCAAG; ATTGACACCATCGCCCCTGACGAGATCACGGTCAGCAGTGACTTCGAGGCTCGCAATGTCAAGCTGAACG; TAGAGGAGCGCATGGTGGGGCCCCTTACCCGGAAGGGCTTCTACCTGGCCTTCCAGGACATCGGCGCCTG; CGTGGCGCTGCTCTCCGTTCGCGTCTACTACAAGAAGTGTCCCGAGATGCTGCAGAGCTTGGCTCGCTTC; CCCGAGACCATTGCTGTCGCTGTCTCCGATACACAACCCCTGGCCACGGTGGCCGGTACCTGCGTGGACC; ATGCCGTGGTGCCTTATGGGGGCGAGGGGCCTCTCATGCACTGCACGGTGGATGGCGAGTGGCTGGTGCC; CATCGGGCAGTGCCTGTGCCAGGAAGGCTACGAGAAGGTCGAGGATGCCTGCCGAGCCTGTTCTCCAGGA; TTCTTCAAGTCTGAGGCATCTGAGAGCCCTTGCCTGGAGTGTCCAGAGCATACCCTGCCATCCACAGAGG; GTGCCACCTCCTGCCAGTGTGAAGAAGGCTATTTCAGGGCACCTGAGGACCCACTGTCCATGTCTTGCAC; ACGTCCACCCTCTGCCCCCAACTACCTCACGGCCATTGGCATGGGTGCCAAAGTAGAACTGCGTTGGACA; GCTCCCAAGGACACTGGTGGCCGCCAGGACATTGTCTACAGTGTCACTTGCGAACAGTGCTGGCCAGAGT; CTGGCGAGTGTGGGCCCTGTGAGGCGAGCGTGCGCTATTCAGAACCTCCTCACGCCCTGACCCGCACGAG; TGTGACAGTCAGTGACCTGGAGCCCCACATGAACTATACCTTCGCTGTCGAAGCACGCAATGGTGTCTCA; GGCCTGGTGACTAGCCGAAGCTTCCGGACTGCCAGCGTCAGTATTAACCAAACAGAGCCCCCCAAAGTGA; GGCTGGAGGACCGAAGCACCACCTCCCTGAGTGTCACCTGGAGCATCCCGGTGTCACAGCAGAGCCGTGT; GTGGAAGTACGAAGTCACCTACCGCAAGAAGGGGGATGCCAACAGCTATAATGTGCGCCGCACGGAAGGC; TTCTCCGTGACCCTGGATGACCTTGCTCCGGATACCACGTACCTGGTGCAGGTGCAGGCGCTGACGCAGG; AGGGCCAGGGAGCCGGCAGCAAAGTGCACGAGTTCCAGACACTGTCCACGGAAGGATCTGCCAACATGGC; GGTGATCGGCGGTGTGGCTGTAGGTGTTGTTTTGCTTCTGGTACTGGCAGGAGTTGGCCTCTTCATCCAT; CGAAGGAGGAGGAACCTGCGGGCTCGCCAGTCCTCTGAGGATGTCCGTTTTTCCAAGTCAGAACAACTAA; AGCCCCTGAAGACCTATGTGGATCCTCACACTTACGAAGACCCCAACCAGGCTGTACTCAAGTTTACCAC; CGAGATCCACCCATCCTGTGTGGCAAGGCAGAAGGTCATTGGAGCAGGAGAGTTTGGAGAGGTCTATAAA; GGGACGCTGAAGGCATCCTCGGGGAAGAAGGAGATACCGGTGGCCATCAAGACACTGAAAGCGGGCTACA; CTGAGAAGCAGCGGGTGGACTTCCTGAGCGAGGCCAGCATCATGGGCCAGTTTAGCCACCACAATATCAT; CCGCCTGGAGGGCGTGGTCTCTAAATACAAACCCATGATGATTATCACAGAGTACATGGAGAATGGAGCG; CTAGACAAGTTCCTTAGGGAGAAGGATGGTGAGTTCAGTGTACTTCAGTTGGTGGGCATGCTGAGGGGTA; TCGCATCCGGCATGAAGTACCTGGCCAACATGAACTACGTGCACCGGGACCTGGCCGCCCGCAACATCCT; CGTCAACAGCAACCTGGTGTGCAAGGTGTCCGATTTTGGCCTGTCGCGTGTGCTGGAAGATGACCCCGAG; GCCACCTACACCACAAGTGGCGGCAAGATCCCTATTCGATGGACAGCCCCAGAGGCCATTTCCTACCGCA; AGTTCACCTCAGCCAGCGATGTGTGGAGCTACGGCATTGTCATGTGGGAAGTGATGACTTATGGCGAACG; GCCCTACTGGGAACTGTCAAACCACGAGGTCATGAAAGCCATCAACGACGGCTTCCGGCTCCCTACGCCC; ATGGACTGCCCTTCAGCCATTTACCAGCTCATGATGCAGTGCTGGCAGCAAGAGCGCTCCCGCCGACCCA; AGTTTGCCGACATCGTTAGCATCCTGGACAAGCTCATCCGAGCCCCCGACTCCCTCAAGACGCTGGCTGA; CTTTGATCCCCGAGTGTCCATCCGGCTGCCCAGCACCAGCGGCTCGGAGGGAGTCCCCTTCCGTACGGTG; TCCGAGTGGCTGGAGAGCATCAAGATGCAACAGTACACGGAACACTTCATGGTGGCTGGCTACACGGCCA; TCGAGAAGGTGGTACAGATGTCCAACGAAGACATCAAAAGGATCGGAGTGCGTCTTCCTGGCCACCAGAA; GCGTATTGCCTACAGCCTGCTGGGACTCAAGGACCAGGTCAACACAGTGGGGATTCCTATC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226151 representing NM_010139; Red=Cloning site Green=Tags(s); ; MELRAVGFCLALLWGCALAAAAAQGKEVVLLDFAAMKGELGWLTHPYGKGWDLMQNIMDDMPIYMYSVCN; VVSGDQDNWLRTNWVYREEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDVDYGTNFQKRQFTK; IDTIAPDEITVSSDFEARNVKLNVEERMVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPEMLQSLARF; PETIAVAVSDTQPLATVAGTCVDHAVVPYGGEGPLMHCTVDGEWLVPIGQCLCQEGYEKVEDACRACSPG; FFKSEASESPCLECPEHTLPSTEGATSCQCEEGYFRAPEDPLSMSCTRPPSAPNYLTAIGMGAKVELRWT; APKDTGGRQDIVYSVTCEQCWPESGECGPCEASVRYSEPPHALTRTSVTVSDLEPHMNYTFAVEARNGVS; GLVTSRSFRTASVSINQTEPPKVRLEDRSTTSLSVTWSIPVSQQSRVWKYEVTYRKKGDANSYNVRRTEG; FSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSTEGSANMAVIGGVAVGVVLLLVLAGVGLFIH; RRRRNLRARQSSEDVRFSKSEQLKPLKTYVDPHTYEDPNQAVLKFTTEIHPSCVARQKVIGAGEFGEVYK; GTLKASSGKKEIPVAIKTLKAGYTEKQRVDFLSEASIMGQFSHHNIIRLEGVVSKYKPMMIITEYMENGA; LDKFLREKDGEFSVLQLVGMLRGIASGMKYLANMNYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPE; ATYTTSGGKIPIRWTAPEAISYRKFTSASDVWSYGIVMWEVMTYGERPYWELSNHEVMKAINDGFRLPTP; MDCPSAIYQLMMQCWQQERSRRPKFADIVSILDKLIRAPDSLKTLADFDPRVSIRLPSTSGSEGVPFRTV; SEWLESIKMQQYTEHFMVAGYTAIEKVVQMSNEDIKRIGVRLPGHQKRIAYSLLGLKDQVNTVGIPI; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010139
• ORF Size: 2931 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_010139.3, NP_034269.2
• RefSeq Size: 3948 bp
• RefSeq ORF: 2934 bp
• Locus ID: 13836
• UniProt ID: Q03145
• Cytogenetics: 4 73.67 cM
• MW: 109.3 kDa
• Summary: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells (PubMed:29749928). Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.[UniProtKB/Swiss-Prot Function]