Epha2 (NM_010139) Mouse Untagged Clone

SKU MC222892

Epha2 (untagged) - Mouse Eph receptor A2 (Epha2), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Epha2
• Synonyms: AW545284; Eck; Myk2; Sek-2; Sek2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_010139, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGAGCTCCGGGCAGTCGGTTTCTGCCTGGCGCTGCTGTGGGGTTGCGCGCTGGCGGCCGCGGCGGCAC; AGGGAAAGGAAGTTGTTTTGTTGGACTTCGCAGCAATGAAGGGAGAGCTCGGCTGGCTCACGCACCCCTA; TGGCAAAGGGTGGGACCTGATGCAGAACATCATGGACGACATGCCTATCTACATGTACTCGGTGTGCAAC; GTGGTATCCGGCGACCAGGACAACTGGCTCCGCACCAACTGGGTGTACCGGGAGGAGGCCGAGCGCATCT; TTATTGAGCTCAAGTTCACGGTGCGAGACTGTAACAGCTTCCCGGGTGGCGCCAGCTCATGCAAAGAGAC; CTTCAACCTCTACTATGCAGAGTCAGATGTGGACTATGGCACCAACTTCCAGAAGCGCCAGTTCACCAAG; ATTGACACCATCGCCCCTGACGAGATCACGGTCAGCAGTGACTTCGAGGCTCGCAATGTCAAGCTGAACG; TAGAGGAGCGCATGGTGGGGCCCCTTACCCGGAAGGGCTTCTACCTGGCCTTCCAGGACATCGGCGCCTG; CGTGGCGCTGCTCTCCGTTCGCGTCTACTACAAGAAGTGTCCCGAGATGCTGCAGAGCTTGGCTCGCTTC; CCCGAGACCATTGCTGTCGCTGTCTCCGATACACAACCCCTGGCCACGGTGGCCGGTACCTGCGTGGACC; ATGCCGTGGTGCCTTATGGGGGCGAGGGGCCTCTCATGCACTGCACGGTGGATGGCGAGTGGCTGGTGCC; CATCGGGCAGTGCCTGTGCCAGGAAGGCTACGAGAAGGTCGAGGATGCCTGCCGAGCCTGTTCTCCAGGA; TTCTTCAAGTCTGAGGCATCTGAGAGCCCTTGCCTGGAGTGTCCAGAGCATACCCTGCCATCCACAGAGG; GTGCCACCTCCTGCCAGTGTGAAGAAGGCTATTTCAGGGCACCTGAGGACCCACTGTCCATGTCTTGCAC; ACGTCCACCCTCTGCCCCCAACTACCTCACGGCCATTGGCATGGGTGCCAAAGTAGAACTGCGTTGGACA; GCTCCCAAGGACACTGGTGGCCGCCAGGACATTGTCTACAGTGTCACTTGCGAACAGTGCTGGCCAGAGT; CTGGCGAGTGTGGGCCCTGTGAGGCGAGCGTGCGCTATTCAGAACCTCCTCACGCCCTGACCCGCACGAG; TGTGACAGTCAGTGACCTGGAGCCCCACATGAACTATACCTTCGCTGTCGAAGCACGCAATGGTGTCTCA; GGCCTGGTGACTAGCCGAAGCTTCCGGACTGCCAGCGTCAGTATTAACCAAACAGAGCCCCCCAAAGTGA; GGCTGGAGGACCGAAGCACCACCTCCCTGAGTGTCACCTGGAGCATCCCGGTGTCACAGCAGAGCCGTGT; GTGGAAGTACGAAGTCACCTACCGCAAGAAGGGGGATGCCAACAGCTATAATGTGCGCCGCACGGAAGGC; TTCTCCGTGACCCTGGATGACCTTGCTCCGGATACCACGTACCTGGTGCAGGTGCAGGCGCTGACGCAGG; AGGGCCAGGGAGCCGGCAGCAAAGTGCACGAGTTCCAGACACTGTCCACGGAAGGATCTGCCAACATGGC; GGTGATCGGCGGTGTGGCTGTAGGTGTTGTTTTGCTTCTGGTACTGGCAGGAGTTGGCCTCTTCATCCAT; CGAAGGAGGAGGAACCTGCGGGCTCGCCAGTCCTCTGAGGATGTCCGTTTTTCCAAGTCAGAACAACTAA; AGCCCCTGAAGACCTATGTGGATCCTCACACTTACGAAGACCCCAACCAGGCTGTACTCAAGTTTACCAC; CGAGATCCACCCATCCTGTGTGGCAAGGCAGAAGGTCATTGGAGCAGGAGAGTTTGGAGAGGTCTATAAA; GGGACGCTGAAGGCATCCTCGGGGAAGAAGGAGATACCGGTGGCCATCAAGACACTGAAAGCGGGCTACA; CTGAGAAGCAGCGGGTGGACTTCCTGAGCGAGGCCAGCATCATGGGCCAGTTTAGCCACCACAATATCAT; CCGCCTGGAGGGCGTGGTCTCTAAATACAAACCCATGATGATTATCACAGAGTACATGGAGAATGGAGCG; CTAGACAAGTTCCTTAGGGAGAAGGATGGTGAGTTCAGTGTACTTCAGTTGGTGGGCATGCTGAGGGGTA; TCGCATCCGGCATGAAGTACCTGGCCAACATGAACTACGTGCACCGGGACCTGGCCGCCCGCAACATCCT; CGTCAACAGCAACCTGGTGTGCAAGGTGTCCGATTTTGGCCTGTCGCGTGTGCTGGAAGATGACCCCGAG; GCCACCTACACCACAAGTGGCGGCAAGATCCCTATTCGATGGACAGCCCCAGAGGCCATTTCCTACCGCA; AGTTCACCTCAGCCAGCGATGTGTGGAGCTACGGCATTGTCATGTGGGAAGTGATGACTTATGGCGAACG; GCCCTACTGGGAACTGTCAAACCACGAGGTCATGAAAGCCATCAACGACGGCTTCCGGCTCCCTACGCCC; ATGGACTGCCCTTCAGCCATTTACCAGCTCATGATGCAGTGCTGGCAGCAAGAGCGCTCCCGCCGACCCA; AGTTTGCCGACATCGTTAGCATCCTGGACAAGCTCATCCGAGCCCCCGACTCCCTCAAGACGCTGGCTGA; CTTTGATCCCCGAGTGTCCATCCGGCTGCCCAGCACCAGCGGCTCGGAGGGAGTCCCCTTCCGTACGGTG; TCCGAGTGGCTGGAGAGCATCAAGATGCAACAGTACACGGAACACTTCATGGTGGCTGGCTACACGGCCA; TCGAGAAGGTGGTACAGATGTCCAACGAAGACATCAAAAGGATCGGAGTGCGTCTTCCTGGCCACCAGAA; GCGTATTGCCTACAGCCTGCTGGGACTCAAGGACCAGGTCAACACAGTGGGGATTCCTATCTGA; ;
• Restriction Sites: SgfI-MluI
• ACCN: NM_010139
• Insert Size: 2934 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC140960, AAI40961
• RefSeq Size: 3218 bp
• RefSeq ORF: 2934 bp
• Locus ID: 13836
• UniProt ID: Q03145
• Cytogenetics: 4 73.67 cM
• Summary: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells (PubMed:29749928). Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.[UniProtKB/Swiss-Prot Function]