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Ghsr (NM_177330) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR226073
Ghsr (Myc-DDK-tagged) - Mouse growth hormone secretagogue receptor (Ghsr)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ghsr
Synonyms C530020I22Rik; Ghsr1a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR226073 representing NM_177330
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGAACGCGACGCCCAGCGAGGAGCCGGAGCCTAACGTCACGCTGGACCTGGACTGGGACGCTTCTC
CCGGCAACGACTCACTCTCTGACGAACTGCTGCCACTGTTCCCCGCGCCGCTGCTGGCGGGCGTCACTGC
CACCTGCGTGGCGCTCTTCGTGGTGGGCATCTCGGGCAACCTGCTCACCATGCTGGTGGTGTCCCGCTTC
CGCGAGCTGCGCACCACCACCAACCTCTACCTATCCAGCATGGCCTTCTCCGATCTGCTCATCTTCCTGT
GCATGCCGCTGGACCTCGTCCGCCTCTGGCAGTATCGGCCCTGGAACTTCGGCGACCTGCTCTGCAAACT
CTTCCAGTTTGTCAGCGAGAGCTGCACCTACGCCACGGTCCTCACCATCACCGCGCTGAGCGTCGAGCGC
TACTTCGCCATCTGCTTCCCGCTGCGGGCCAAGGTGGTGGTCACCAAGGGCCGTGTGAAGCTGGTCATCC
TTGTCATTTGGGCCGTGGCCTTCTGCAGCGCGGGGCCCATCTTCGTGCTGGTGGGCGTGGAGCACGAGAA
CGGCACAGATCCCCGGGACACCAACGAGTGCCGCGCCACCGAGTTCGCTGTGCGCTCTGGGCTGCTCACC
GTGATGGTATGGGTGTCGAGCGTCTTCTTCTTCCTGCCGGTCTTCTGCCTCACTGTGCTCTACAGTCTCA
TCGGGAGGAAGCTGTGGCGGAGGCGCGGCGACGCGGCGGTGGGCTCCTCGCTCAGGGACCAGAACCACAA
ACAGACAGTGAAGATGCTTGCTGTGGTGGTGTTTGCTTTCATCCTCTGCTGGCTGCCCTTCCACGTGGGA
AGATATCTGTTTTCCAAGTCTTTCGAGCCTGGCTCTCTGGAGATCGCGCAGATCAGTCAGTACTGCAACC
TGGTGTCCTTTGTCCTCTTCTACCTCAGCGCTGCCATCAACCCCATTCTCTACAACATCATGTCCAAGAA
GTACCGGGTGGCCGTGTTCAAACTTCTAGGATTTGAATCCTTCTCCCAGAGAAAGCTTTCCACTCTGAAG
GATGAGAGTTCCCGGGCCTGGACAAAGTCGAGCATCAATACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177330
ORF Size 1092 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177330.4, NP_796304.1
RefSeq Size 4433 bp
RefSeq ORF 1095 bp
Locus ID 208188
UniProt ID Q99P50
Cytogenetics 3 A3
MW 41.4 kDa
Summary Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Binds also other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine) (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177330" in other vectors (4)
SKU Description Size Price
MC212500 Ghsr (untagged) - Mouse growth hormone secretagogue receptor (Ghsr), (10ug) 10 ug
$457.00
MG226073 Ghsr (tGFP-tagged) - Mouse growth hormone secretagogue receptor (Ghsr), (10ug) 10 ug
$657.00
MR226073L3 Lenti ORF clone of Ghsr (Myc-DDK-tagged) - Mouse growth hormone secretagogue receptor (Ghsr) 10 ug
$757.00
MR226073L4 Lenti ORF clone of Ghsr (mGFP-tagged) - Mouse growth hormone secretagogue receptor (Ghsr) 10 ug
$757.00

Plasmid Map for MR226073

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR226073
Circular map for MR226073

file-alt Citations

Internal-state-dependent control of feeding behavior via hippocampal ghrelin signaling
Wee, Mishchanchuk, AlSubaie et al
Neuron (2023) 112 (2), 288-305.e7 DOI: 10.1016/j.neuron.2023.10.016
Cited by 46
AI Summary: In this study, the mouse GHSR1a receptor was expressed using an N-terminal FLAG-tagged vector from OriGene (Cat# MR226073) to investigate how ghrelin signaling in the hippocampus influences feeding behavior.
Snippet: Mouse GHSR1a receptor was expressed with an N-terminal FLAG tag using a vector purchased from Origene (Cat# MR226073). For expression
Showing 1-1 of 1 papers.
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