Ghsr (NM_177330) Mouse Untagged Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Ghsr |
| Synonyms | C530020I22Rik; Ghsr1a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI | |
| ACCN | NM_177330 |
| Insert Size | 1095 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC120672, AAI20673 |
| RefSeq Size | 1188 bp |
| RefSeq ORF | 1095 bp |
| Locus ID | 208188 |
| UniProt ID | Q99P50 |
| Cytogenetics | 3 A3 |
| Summary | Receptor for ghrelin, coupled to G-alpha-11 proteins. Stimulates growth hormone secretion. Binds also other growth hormone releasing peptides (GHRP) (e.g. Met-enkephalin and GHRP-6) as well as non-peptide, low molecular weight secretagogues (e.g. L-692,429, MK-0677, adenosine) (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_177330" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG226073 | Ghsr (tGFP-tagged) - Mouse growth hormone secretagogue receptor (Ghsr), (10ug) | 10 ug |
$657.00
|
|
| MR226073 | Ghsr (Myc-DDK-tagged) - Mouse growth hormone secretagogue receptor (Ghsr) | 10 ug |
$289.00
$457.00
|
|
| MR226073L3 | Lenti ORF clone of Ghsr (Myc-DDK-tagged) - Mouse growth hormone secretagogue receptor (Ghsr) | 10 ug |
$757.00
|
|
| MR226073L4 | Lenti ORF clone of Ghsr (mGFP-tagged) - Mouse growth hormone secretagogue receptor (Ghsr) | 10 ug |
$757.00
|
|
Citations
Metabolic insights from a GHSR-A203E mutant mouse model
Torz, Osborne-Lawrence, Rodriguez et al
Mol Metab (2020) 39, 101004 DOI: 10.1016/j.molmet.2020.101004
Torz, Osborne-Lawrence, Rodriguez et al
Mol Metab (2020) 39, 101004 DOI: 10.1016/j.molmet.2020.101004
Cited by 47
AI Summary: OriGene's mouse GHSR plasmid (MC212500) was used as the template to generate the GHSR-A203E mutant construct, enabling the study of this mutation's effects on receptor function and metabolism in the described research.
Snippet: mbe and GHSR-WT was kindly provided by Dr. Jacky Marie, University of Montpellier, Montpellier, France). The GHSR-A203E construct was generated through directed mutagenesis of a mouse GHSR (MC212500, OriGene, Rockville, MD, USA) containing plasmid by Dr. Silvia Rodriguez at the IMBICE, La Plata. For experiments lacking one or more plasmids, the cells were transfected with empty plasmid pcDNA3.1 (+;CaV subunits were kindly provided by Dr. Lipscombe and GHSR-WT was kindly provided by Dr. Jacky Marie, Université de Montpellier, France). The GHSR-A203E construct was generated through directed mutagenesis of a mouse GHSR (MC212500, Origene, Rockville)-containing plasmid by Dr. Silvia Rodriguez at the IMBICE, La Plata. …
Showing 1-1 of 1 papers.
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