Sp1 (NM_013672) Mouse Tagged ORF Clone
$589.00
$715.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Sp1 |
| Synonyms | 1110003E12Rik; AA450830; AI845540; Sp1-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_013672 |
| ORF Size | 2343 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_013672.2, NP_038700.2 |
| RefSeq Size | 7818 bp |
| RefSeq ORF | 2346 bp |
| Locus ID | 20683 |
| UniProt ID | O89090 |
| Cytogenetics | 15 F3 |
| MW | 80.4 kDa |
| Summary | Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter Plays an essential role in the regulation of FE65 gene expression (By similarity). Positively regulates the transcription of the core clock component ARNTL/BMAL1 (PubMed:24030830). Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (PubMed:27918959).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_013672" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC221564 | Sp1 (untagged) - Mouse trans-acting transcription factor 1 (Sp1), (10ug) | 10 ug |
$716.00
|
|
| MG225692 | Sp1 (tGFP-tagged) - Mouse trans-acting transcription factor 1 (Sp1), (10ug) | 10 ug |
$915.00
|
|
| MR225692L3 | Lenti ORF clone of Sp1 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 1 (Sp1) | 10 ug |
$1,015.00
|
|
| MR225692L4 | Lenti ORF clone of Sp1 (mGFP-tagged) - Mouse trans-acting transcription factor 1 (Sp1) | 10 ug |
$1,015.00
|
|
Citations
Indole induces intestinal T helper-17 cell overproduction during inflammatory bowel disease through PKA/SP1-mediated Trim21 transcription
Wu, Ma, Zeng et al
BMC Medicine (2026)
Wu, Ma, Zeng et al
BMC Medicine (2026)
Snippet: PCMV6-SP1-Myc-Flag (MR225692), and PCMV6-PRKACA-Myc-Flag (MR205322) were purchased from Origene Technologies (Rockwell, Maryland, USA). The PCMV6-Fbxw7- His, PCMV6-Trim21-Flag, PCMV6-TΔR-Flag, PCMV6-SP1-Flag, PCMV6-SP1-T453A-Flag and PCMV6- PRKACA-Myc plasmids were constructed from the above plasmids
Regulation of the RhoA exchange factor GEF-H1 by pro-fibrotic stimuli through a positive feedback loop involving RhoA, MRTF and Sp1
Venugopal, Dan, Sri Theivakadadcham et al
Am J Physiol Cell Physiol (2024)
Venugopal, Dan, Sri Theivakadadcham et al
Am J Physiol Cell Physiol (2024)
Cited by 11
Downregulation of Sp1 by Minnelide leads to decrease in HSP70 and decrease in tumor burden of gastric cancer
Arora, Alsaied, Dauer et al
PLoS ONE (2017) 12 (2), e0171827 DOI: 10.1371/journal.pone.0171827
Arora, Alsaied, Dauer et al
PLoS ONE (2017) 12 (2), e0171827 DOI: 10.1371/journal.pone.0171827
AI Summary: OriGene’s pCMV-Sp1 plasmid was used to overexpress Sp1 in gastric cancer cell lines, enabling the study to assess how Sp1 modulation by Minnelide affects HSP70 levels and tumor burden.
Snippet: 2.9 Overexpression of Sp1. Sp1 was overexpressd in MKN28 and MKN45 cell lines pCMV-Sp1 (Origene). Sp1 overexpressing cells were plated and treated with 100nM triptolide to assess viability as described above. Non-overexpressing cells served as control. ... All 5 versions Cite SaveSaving
Showing 1-3 of 3 papers.
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