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Sp1 (NM_013672) Mouse Untagged Clone

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SKU
MC221564
Sp1 (untagged) - Mouse trans-acting transcription factor 1 (Sp1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $716.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Sp1
Synonyms 1110003E12Rik; AA450830; AI845540; Sp1-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC221564 representing NM_013672
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGACCAAGATCACTCCATGGATGAAGTGACAGCTGTGGTGAAGATTGAAAAAGATGTTGGTGGCA
ATAATGGGGGTAGCGGCAATGGTGGCGGTGCCGCCTTTTCTCAGACTCGAAGCAGCAGCACAGGCAGTAG
CAGCAGCAGTGGTGGCGGAGGAGGGCAGGAATCCCAGCCATCTCCTTTGGCTCTGCTGGCAGCAACCTGC
AGCAGAATTGAGTCACCCAATGAGAACAGCAACAACTCCCAGGGTCCGAGTCAGTCAGGGGGCACAGGTG
AACTTGACCTCACAGCCGCACAACTTTCACAGGGTGCCAATGGCTGGCAGATCATCTCTTCCTCCTCTGG
GGCTACCCCTACCTCAAAGGAACAGAGTGGCAACAGTACCAATGGCAGCGAGTCTTCCAAGAACCGCACA
GTCTCTGGTGGGCAGTATGTTGTGGCTGCTACCCCCAACTTACAGAACCAGCAAGTTCTGACAGGTCTCC
CTGGAGTAATGCCTAATATTCAGTATCAAGTAATCCCACAGTTCCAGACTGTTGATGGGCAGCAGCTGCA
GTTTGCTGCCACTGGGGCCCAAGTGCAGCAGGATGGTTCTGGTCAAATACAGATCATACCAGGTGCAAAC
CAACAGATCATCCCAAATAGAGGAAGTGGGGGCAACATTATTGCTGCTATGCCAAATCTACTCCAGCAGG
CTGTCCCCCTTCAAGGCCTTGCTAATAATGTGCTCTCAGGACAGACTCAGTATGTGACCAATGTACCAGT
GGCCCTGAATGGGAACATCACCTTGCTACCTGTCAACAGCGTTTCTGCAGCTACCCTGACTCCCAGCTCT
CAGGCAGGCACTATCAGCAGCTCTGGATCCCAGGAGAGCAGCTCACAGCCTGTCACCTCAGGGACTGCCA
TCAGTTCTGCCAGCTTGGTGTCATCACAAGCTAGTTCCAGCTCCTTTTTTACCAATGCCAATAGTTATTC
AACAACTACTACCACCAGCAACATGGGAATTATGAACTTTACCAGCAGTGGCTCATCAGGGACTAGTTCT
CAAGGCCAGACGCCCCAGAGGGTTGGTGGGCTACAAGGGTCTGATTCTCTGAACATCCAGCAGAACCAGA
CATCAGGAGGCTCGCTGCAAGGAAGTCAGCAGAAAGAGGGAGAGCAAAGTCAGCAGACACAGCAACAACA
AATCCTTATTCAGCCTCAGCTAGTTCAAGGAGGACAAGCTCTTCAGGCCCTTCAAGCAGCACCATTGTCC
GGACAGACCTTCACAACTCAAGCTATTTCCCAGGAAACCCTTCAGAACCTCCAGCTTCAGGCTGTTCAAA
ACTCTGGTCCCATCATCATTCGGACACCAACCGTGGGGCCCAATGGACAGGTCAGTTGGCAGACCCTTCA
GCTGCAGAATCTTCAAGTTCAGAACCCACAAGCCCAGACAATCACCTTGGCCCCTATGCAGGGTGTTTCC
TTGGGGCAGACTAGCAGCAGTAATACCACCCTAACACCCATTGCCTCAGCTGCCTCCATTCCTGCTGGCA
CAGTCACTGTGAATGCTGCTCAACTCTCCTCCATGCCAGGCCTCCAGACCATTAACCTCAGTGCATTGGG
TACTTCAGGGATCCAGGTGCACCAGCTTCCAGGCCTGCCTTTGGCTATAGCAAACACCCCAGGTGATCAT
GGAACTCAACTTGGTCTTCATGGATCTGGTGGTGATGGGATACATGATGAGACAGCAGGTGGAGAAGGAG
AGAATAGCTCTGATCTCCAACCCCAAGCTGGACGCAGGACTCGTCGGGAAGCATGTACATGCCCCTATTG
CAAAGACAGTGAGGGAAGAGCCTCAGGAGATCCTGGCAAAAAGAAACAGCACATTTGTCACATCCAAGGA
TGCGGCAAAGTATATGGCAAGACCTCACATCTCCGAGCACACTTGCGCTGGCATACAGGGGAGAGGCCAT
TCATGTGTAATTGGTCATATTGTGGGAAGCGCTTTACACGTTCGGACGAGCTTCAGAGACATAAACGTAC
ACATACAGGAGAGAAGAAATTTGCCTGCCCTGAGTGCCCTAAGCGTTTCATGAGGAGTGATCACCTGTCA
AAGCATATCAAGACTCACCAGAACAAGAAGGGAGGCCCAGGTGTAGCCCTGAGTGTGGGCACATTGCCCC
TGGACAGTGGGGCAGGTTCAGAAGGCACTGCCACTCCTTCAGCCCTTATTACCACCAATATGGTAGCCAT
GGAGGCCATCTGTCCAGAGGGTATTGCCCGTCTTGCCAACAGTGGCATCAACGTCATGCAGGTGACAGAG
CTGCAGTCCATTAATATCAGTGGCAATGGTTTCTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN NM_013672
Insert Size 2346 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013672.2, NP_038700.2
RefSeq Size 7818 bp
RefSeq ORF 2346 bp
Locus ID 20683
UniProt ID O89090
Cytogenetics 15 F3
Summary Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter Plays an essential role in the regulation of FE65 gene expression (By similarity). Positively regulates the transcription of the core clock component ARNTL/BMAL1 (PubMed:24030830). Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (PubMed:27918959).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013672" in other vectors (4)
SKU Description Size Price
MG225692 Sp1 (tGFP-tagged) - Mouse trans-acting transcription factor 1 (Sp1), (10ug) 10 ug
$915.00
MR225692 Sp1 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 1 (Sp1) 10 ug
$589.00 $715.00
MR225692L3 Lenti ORF clone of Sp1 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 1 (Sp1) 10 ug
$1,015.00
MR225692L4 Lenti ORF clone of Sp1 (mGFP-tagged) - Mouse trans-acting transcription factor 1 (Sp1) 10 ug
$1,015.00

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