Trpc6 (NM_013838) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR225311
Trpc6 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 6 (Trpc6)
$1,277.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Trpc6 |
| Synonyms | AV025995; LLHWJM002; LLHWJM003; LLHWJM004; mtrp6; TRP-6; Trrp6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_013838 |
| ORF Size | 2790 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_013838.2, NP_038866.2 |
| RefSeq Size | 3259 bp |
| RefSeq ORF | 2793 bp |
| Locus ID | 22068 |
| UniProt ID | Q61143, Q3UZG1 |
| Cytogenetics | 9 2.46 cM |
| MW | 107.1 kDa |
| Summary | Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_013838" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC222685 | Trpc6 (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 6 (Trpc6), (10ug) | 10 ug |
$1,363.00
|
|
| MG225311 | Trpc6 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily C member 6 (Trpc6), (10ug) | 10 ug |
$1,477.00
|
|
| MR225311L1 | Lenti ORF clone of Trpc6 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 6 (Trpc6) | 10 ug |
$1,577.00
|
|
| MR225311L2 | Lenti ORF clone of Trpc6 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 6 (Trpc6) | 10 ug |
$1,577.00
|
|
| MR225311L3 | Lenti ORF clone of Trpc6 (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 6 (Trpc6) | 10 ug |
$1,577.00
|
|
| MR225311L4 | Lenti ORF clone of Trpc6 (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 6 (Trpc6) | 10 ug |
$1,577.00
|
|
Citations
Insulin increases filtration barrier permeability via TRPC6-dependent activation of PKGIα signaling pathways
Rogacka, Audzeyenka, Rachubik et al
Biochim. Biophys. Acta (2017)
Rogacka, Audzeyenka, Rachubik et al
Biochim. Biophys. Acta (2017)
Snippet: 2.7. RNA interference and cell transfection. We transfected podocytes with small interfering RNAs (siRNA) that targeted PKGIα, NOX4, NOX2 (Santa Cruz Biotechnology), TRPC6 (Origene) and a control, non-silencing siRNA (scrambled siRNA, negative control). ... All 2 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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