Larp1 (NM_028451) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR224022
Larp1 (Myc-DDK-tagged) - Mouse La ribonucleoprotein domain family, member 1 (Larp1). Note: ORF is codon optimized
$589.00
$933.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Larp1 |
| Synonyms | 1810024J12Rik; 3110040D16Rik; Larp; mKIAA0731 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_028451 |
| ORF Size | 3216 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_028451.1, NP_082727.1 |
| RefSeq Size | 6617 bp |
| RefSeq ORF | 3219 bp |
| Locus ID | 73158 |
| UniProt ID | Q6ZQ58 |
| Cytogenetics | 11 B1.3 |
| MW | 121.1 kDa |
| Summary | RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability. Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery. The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1. When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA. Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif. Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes. Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis. Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation. Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_028451" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC223454 | Larp1 (untagged) - Mouse La ribonucleoprotein domain family, member 1 (Larp1), (10ug) | 10 ug |
$1,027.00
|
|
| MG224022 | Larp1 (tGFP-tagged) - Mouse La ribonucleoprotein domain family member 1 (Larp1), (10ug). Note: ORF is codon optimized | 10 ug |
$1,133.00
|
|
| MR224022L3 | Lenti ORF clone of Larp1 (Myc-DDK-tagged) - Mouse La ribonucleoprotein domain family, member 1 (Larp1). Note: ORF is codon optimized | 10 ug |
$1,233.00
|
|
| MR224022L4 | Lenti ORF clone of Larp1 (mGFP-tagged) - Mouse La ribonucleoprotein domain family, member 1 (Larp1). Note: ORF is codon optimized | 10 ug |
$1,233.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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