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Pfn4 (NM_028376) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223450
Pfn4 (Myc-DDK-tagged) - Mouse profilin family, member 4 (Pfn4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Pfn4 or NM_028376"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pfn4
Synonyms 2900024P18Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223450 representing NM_028376
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTCACTTGCAGAACTTACTGTTAGATACCCTGCTGGGGACGAAACACGTGGACAGCGCAGCCCTCA
TCAAACTCCAGGAAAAGACCCTGTGTGTGACATCACCAGGATTTAGTGTAATGCCAAGTGATGTTCGAAC
ACTTCTGAATGGATTTGCCAAAAACCCATTATTAACGCGAAGGGAAGGGTTGTATTTCAAGGAGAAGGAT
TACAAATGTGTTCGGGCAGATGACTATTCTCTCTATGCTAAGAATGAGAACACTGGTGTGGTGGTGGTGA
AGACCAACATGTATCTTGTGGTGGCAACTTACACTGCAGGCATGTATCCTAGCGTCTGCGTGGAAGCCAC
AGAGAAGCTGGGAGAGTATCTAAGGAAAAAGGGAAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223450 representing NM_028376
Red=Cloning site Green=Tags(s)

MSHLQNLLLDTLLGTKHVDSAALIKLQEKTLCVTSPGFSVMPSDVRTLLNGFAKNPLLTRREGLYFKEKD
YKCVRADDYSLYAKNENTGVVVVKTNMYLVVATYTAGMYPSVCVEATEKLGEYLRKKGN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028376
ORF Size 387 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028376.4
RefSeq Size 1306 bp
RefSeq ORF 390 bp
Locus ID 382562
UniProt ID Q6JEK6, Q9D6I3
Cytogenetics 12 A1.1
MW 14.4 kDa
Summary Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), phosphatidylinositol 4-phosphate (PtdIns(4)P) and phosphatidic acid (PA). Does not bind to actin, contrary to other family members (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_028376" in other vectors (4)
SKU Description Size Price
MC214771 Pfn4 (untagged) - Mouse profilin family, member 4 (Pfn4), (10ug) 10 ug
$165.00
MG223450 Pfn4 (tGFP-tagged) - Mouse profilin family member 4 (Pfn4), (10ug) 10 ug
$350.00
MR223450L3 Lenti ORF clone of Pfn4 (Myc-DDK-tagged) - Mouse profilin family, member 4 (Pfn4) 10 ug
$450.00
MR223450L4 Lenti ORF clone of Pfn4 (mGFP-tagged) - Mouse profilin family, member 4 (Pfn4) 10 ug
$450.00

Plasmid Map for MR223450

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223450
Circular map for MR223450

file-alt Citations

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