Pfn4 (NM_028376) Mouse Untagged Clone

SKU
MC214771
Pfn4 (untagged) - Mouse profilin family, member 4 (Pfn4), (10ug)
  $165.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pfn4
Synonyms 2900024P18Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC214771 representing NM_028376
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTCACTTGCAGAACTTACTGTTAGATACCCTGCTGGGGACGAAACACGTGGACAGCGCAGCCCTCA
TCAAACTCCAGGAAAAGACCCTGTGTGTGACATCACCAGGATTTAGTGTAATGCCAAGTGATGTTCGAAC
ACTTCTGAATGGATTTGCCAAAAACCCATTATTAACGCGAAGGGAAGGGTTGTATTTCAAGGAGAAGGAT
TACAAATGTGTTCGGGCAGATGACTATTCTCTCTATGCTAAGAATGAGAACACTGGTGTGGTGGTGGTGA
AGACCAACATGTATCTTGTGGTGGCAACTTACACTGCAGGCATGTATCCTAGCGTCTGCGTGGAAGCCAC
AGAGAAGCTGGGAGAGTATCTAAGGAAAAAGGGAAACTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_028376
Insert Size 390 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028376.3, NP_082652.1
RefSeq Size 1306 bp
RefSeq ORF 390 bp
Locus ID 382562
UniProt ID Q9D6I3
Cytogenetics 12 A1.1
Summary Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), phosphatidylinositol 4-phosphate (PtdIns(4)P) and phosphatidic acid (PA). Does not bind to actin, contrary to other family members (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_028376" in other vectors (4)
SKU Description Size Price
MG223450 Pfn4 (tGFP-tagged) - Mouse profilin family member 4 (Pfn4), (10ug) 10 ug
$350.00
MR223450 Pfn4 (Myc-DDK-tagged) - Mouse profilin family, member 4 (Pfn4) 10 ug
$289.00
MR223450L3 Lenti ORF clone of Pfn4 (Myc-DDK-tagged) - Mouse profilin family, member 4 (Pfn4) 10 ug
$450.00
MR223450L4 Lenti ORF clone of Pfn4 (mGFP-tagged) - Mouse profilin family, member 4 (Pfn4) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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