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Cntnap1 (NM_016782) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR223061
Cntnap1 (Myc-DDK-tagged) - Mouse contactin associated protein-like 1 (Cntnap1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $1,185.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cntnap1
Synonyms AI841080; Caspr; NCP1; Nrxn4; p190; shm
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR223061 representing NM_016782
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGAGTCTCCGGCTCTTCAGCATCCTGCTCGCCACGGTGGTCTCTGGAGCTTGGGGCTGGGGCTACT
ACGGTTGCAATGAGGAGCTGGTGGGGCCTCTGTATGCACGGTCTCTGGGCGCTTCTTCCTACTATGGACT
CTTTACCACAGCCCGCTTTGCCCGGCTACATGGCATCAGTGGATGGTCGCCCCGGATTGGGGACCCGAAT
CCCTGGCTGCAGATCGACTTAATGAAGAAGCATCGGATCCGGGCTGTGGCCACACAGGGAGCTTTTAATT
CTTGGGATTGGGTCACACGTTACATGCTGCTCTACGGGGACCGTGTGGACAGCTGGACACCATTCTACCA
AAAAGGGCACAATGCGACCTTCTTCGGGAATGTCAATGACTCGGCAGTGGTCCGCCATGACCTTCACTAC
CACTTTACGGCTCGCTACATCCGCATCGTGCCACTGGCCTGGAACCCACGCGGCAAGATTGGCCTGAGGC
TGGGCATCTATGGTTGTCCCTACACATCCAGCATCCTGTATTTTGACGGCGACGATGCCATCTCATACCG
CTTCCAGCGAGGCGCCAGCCAAAGTCTTTGGGACGTGTTCGCTTTTAGTTTCAAGACAGAGGAGAAGGAT
GGGCTGTTGCTGCACACCGAGGGCTCCCAGGGGGATTATGTGACGCTTGAACTGCAAGGGGCGCACCTGC
TGCTGCACATGAGCCTGGGCAGCAGTCCCATCCAGCCAAGACCTGGTCACACCACGGTGAGCCTTGGTGG
CGTTCTTAACGACCTAAGCTGGCACTATGTGCGGGTGGATCGATATGGCCGAGATGCAAATTTCACCCTG
GATGGTTACGCCCATCACTTTGTGCTCAACGGCGACTTTGAAAGGCTGAATCTTGAAAATGAGATATTCA
TCGGGGGTCTAGTGGGCGCAGCCCGTAAGAACCTGGCCTACCGCCATAACTTCCGTGGCTGCATAGAAAA
CGTGATCTACAACCGGATCAACATTGCAGAAATGGCAGTGATGCGCCATTCGCGGATCACCTTTGAGGGT
AATGTGGCTTTCCGTTGCTTGGATCCCGTTCCACACCCCATCAACTTCGGAGGCCCTCACAACTTCGTCC
AAGTGCCTGGCTTTCCACGCCGAGGACGCTTAGCCGTCTCTTTTCGTTTCCGCACCTGGGACCTCACAGG
GCTGCTCCTTTTCTCCCACTTGGGGGACGGGCTGGGTCATGTGGAGCTGATGCTTAGCGAAGGGCAAGTG
AATGTATCCATCGCGCAGACTGGCCGCAAGAAGCTTCAGTTTGCTGCTGGGTACCGCCTGAATGATGGCT
TCTGGCACGAGGTGAACTTTGTGGCACAGGAAAACCATGCAGTCATCAGTATTGATGATGTGGAAGGGGC
AGAGGTCAGGGTTTCATACCCACTGCTGATCCGCACAGGGACTTCATACTTCTTTGGTGGTTGTCCCAAA
CCAGCCAGTCGATGGGGCTGCCACTCCAACCAGACAGCATTCCATGGCTGCATGGAGCTGCTCAAGGTGG
ACGGTCAACTGGTCAACCTCACTCTGGTAGAGTTTCGGAAGCTCGGTTATTTTGCTGAGGTCCTCTTTGA
CACATGTGGCATCACAGACAGATGCAGCCCTAACATGTGTGAGCATGACGGACGATGCTACCAGTCTTGG
GATGACTTCATCTGCTACTGCGAACTTACCGGCTACAAGGGAGTTACCTGCCACGAACCATTGTACAAGG
AGTCCTGTGAGGCCTATCGGCTCAGTGGGAAATATTCTGGAAACTACACCATTGATCCTGATGGCAGTGG
ACCCCTGAAGCCGTTTGTGGTGTATTGTGACATCCGAGAGAACCGAGCGTGGACAGTTGTGCGGCATGAC
AGGCTGTGGACCACTCGAGTGACTGGTTCCAGCATGGACCGGCCCTTTCTGGGGGCCATCCAATACTGGA
ATGCCTCCTGGGAGGAAGTCAGCGCTCTGGCCAATGCTTCCCAACACTGTGAGCAGTGGATCGAGTTTTC
CTGCTACAATTCCCGGCTGCTCAACACTGCAGGAGGCTACCCCTACAGCTTTTGGATTGGCCGCAATGAG
GAACAGCATTTCTACTGGGGAGGCTCCCAGCCTGGGATCCAGCGCTGTGCCTGTGGGCTGGACCAGAGCT
GTGTGGACCCTGCACTGCACTGCAATTGTGATGCCGACCAGCCACAGTGGAGAACAGACAAGGGGCTCCT
GACCTTTGTAGACCATCTGCCTGTCACTCAGGTAGTGGTAGGTGATACAAACCGCTCAAATTCTGAAGCT
CAGTTCTTCCTGAGGCCTCTGCGCTGCTATGGTGACCGCAATTCCTGGAACACCATCTCCTTCCACACTG
GAGCTGCACTGCGTTTCCCTCCGATCCGAGCCAACCACAGCCTCGATGTCTCATTCTACTTCAGGACCTC
GGCTCCCTCGGGTGTCTTCCTAGAGAACATGGGGGGTCCTTTCTGCCGGTGGCGCCGACCTTACGTGAGA
GTGGAGCTCAACACATCCCGGGATGTGGTCTTTGCCTTTGATATTGGCAATGGGGATGAGAACCTGACAG
TGCACTCGGATGACTTTGAGTTTAACGATGATGAGTGGCATTTGGTCCGAGCTGAAATCAACGTGAAGCA
GGCCCGGCTGCGAGTGGATCACCGGCCCTGGGTGCTAAGGCCCATGCCCCTGCAGACCTACATCTGGCTG
GTGTATGACCAACCCCTCTATGTGGGATCTGCAGAGCTTAAGAGGCGCCCTTTTGTGGGCTGCTTGAGGG
CCATGCGTTTGAATGGAGTGACTCTGAACTTGGAGGGCCGTGCCAATGCCTCTGAGGGCACCTTCCCCAA
CTGCACCGGCCACTGCACCCACCCCCGGTTCCCCTGTTTCCACGGAGGACGCTGTGTGGAGCGATACAGC
TACTACACATGTGACTGTGACCTCACAGCTTTTGATGGGCCATACTGCAATCACGATATTGGTGGATTCT
TTGAGACCGGCACATGGATGCGCTATAACCTCCAGTCAGCACTGCGCTCTGCAGCCCGGGAGTTCTCACA
CATGCTGAGCCGGCCAGTGCCGGGCTATGAGCCTGGCTATGTTCCAGGCTACGACACTCCTGGTTACGTG
CCTGGATACCACGGCCCTGGGTACCGACTGCCCGAGTACCCGAGGCCCGGCCGGCCAGTGCCCGGGTATA
GGGGGCCTGTCTACAATGTTACTGGAGAGGAGGTCTCCTTCAGCTTCAGCACCAACTCTGCTCCTGCCGT
CCTGCTCTATGTCAGCTCCTTTGTACGTGACTACATGGCTGTGCTCATCAAGGAAGACGGGACCCTACAG
CTTCGGTATCAGTTGGGCACCAGTCCCTATGTGTACCAGCTAACCACCCGGCCAGTGACTGATGGTCAGC
CCCATAGTGTCAACATCACCCGGGTCTACCGAAACCTCTTTATCCAGGTGGACTACTTCCCGCTGACAGA
ACAGAAGTTCTCTCTCCTGGTGGACAGCCAGCTGGACTCACCCAAGGCCTTGTATCTAGGGCGTGTGATG
GAGACAGGAGTCATTGACCCAGAGATTCAGCGGTACAACACCCCAGGTTTCTCAGGCTGCCTGTCTGGTG
TCCGGTTCAACAATGTGGCTCCTCTCAAGACCCATTTCCGAACCCCTCGGCCCATGACTGCTGAGCTGGC
GGAGGCCATGCGGGTTCAAGGAGAACTGTCAGAATCTAACTGTGGAGCTATGCCACGCCTTGTCTCTGAG
GTGCCACCAGAACTTGATCCCTGGTACCTGCCTCCAGATTTCCCATACTACCATGACGACGGATGGATTG
CCATACTCTTAGGTTTTTTGGTGGCCTTCCTGCTGCTGGGGCTTGTGGGAATGCTGGTGCTGTTCTATCT
GCAAAATCATCGATACAAGGGCTCCTACCACACCAACGAGCCCAAGGCCACCCACGATTCCCATCCTGGC
GGCAAAGCTCCCCTCCCTCCCTCAGGCCCTGCCCAGGCCCCTGCCCCTACTCCAGCTCCCACACAGCTTC
CAACCCCAGCCCCAGCCCCAGCCCCAGCCCCAGCCTCTGGCCCAGGCCCCAGAGACCAGAACCTCCCCCA
GATCTTGGAGGAGTCCAGGTCTGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR223061 representing NM_016782
Red=Cloning site Green=Tags(s)

MMSLRLFSILLATVVSGAWGWGYYGCNEELVGPLYARSLGASSYYGLFTTARFARLHGISGWSPRIGDPN
PWLQIDLMKKHRIRAVATQGAFNSWDWVTRYMLLYGDRVDSWTPFYQKGHNATFFGNVNDSAVVRHDLHY
HFTARYIRIVPLAWNPRGKIGLRLGIYGCPYTSSILYFDGDDAISYRFQRGASQSLWDVFAFSFKTEEKD
GLLLHTEGSQGDYVTLELQGAHLLLHMSLGSSPIQPRPGHTTVSLGGVLNDLSWHYVRVDRYGRDANFTL
DGYAHHFVLNGDFERLNLENEIFIGGLVGAARKNLAYRHNFRGCIENVIYNRINIAEMAVMRHSRITFEG
NVAFRCLDPVPHPINFGGPHNFVQVPGFPRRGRLAVSFRFRTWDLTGLLLFSHLGDGLGHVELMLSEGQV
NVSIAQTGRKKLQFAAGYRLNDGFWHEVNFVAQENHAVISIDDVEGAEVRVSYPLLIRTGTSYFFGGCPK
PASRWGCHSNQTAFHGCMELLKVDGQLVNLTLVEFRKLGYFAEVLFDTCGITDRCSPNMCEHDGRCYQSW
DDFICYCELTGYKGVTCHEPLYKESCEAYRLSGKYSGNYTIDPDGSGPLKPFVVYCDIRENRAWTVVRHD
RLWTTRVTGSSMDRPFLGAIQYWNASWEEVSALANASQHCEQWIEFSCYNSRLLNTAGGYPYSFWIGRNE
EQHFYWGGSQPGIQRCACGLDQSCVDPALHCNCDADQPQWRTDKGLLTFVDHLPVTQVVVGDTNRSNSEA
QFFLRPLRCYGDRNSWNTISFHTGAALRFPPIRANHSLDVSFYFRTSAPSGVFLENMGGPFCRWRRPYVR
VELNTSRDVVFAFDIGNGDENLTVHSDDFEFNDDEWHLVRAEINVKQARLRVDHRPWVLRPMPLQTYIWL
VYDQPLYVGSAELKRRPFVGCLRAMRLNGVTLNLEGRANASEGTFPNCTGHCTHPRFPCFHGGRCVERYS
YYTCDCDLTAFDGPYCNHDIGGFFETGTWMRYNLQSALRSAAREFSHMLSRPVPGYEPGYVPGYDTPGYV
PGYHGPGYRLPEYPRPGRPVPGYRGPVYNVTGEEVSFSFSTNSAPAVLLYVSSFVRDYMAVLIKEDGTLQ
LRYQLGTSPYVYQLTTRPVTDGQPHSVNITRVYRNLFIQVDYFPLTEQKFSLLVDSQLDSPKALYLGRVM
ETGVIDPEIQRYNTPGFSGCLSGVRFNNVAPLKTHFRTPRPMTAELAEAMRVQGELSESNCGAMPRLVSE
VPPELDPWYLPPDFPYYHDDGWIAILLGFLVAFLLLGLVGMLVLFYLQNHRYKGSYHTNEPKATHDSHPG
GKAPLPPSGPAQAPAPTPAPTQLPTPAPAPAPAPASGPGPRDQNLPQILEESRSE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016782
ORF Size 4155 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016782.2, NP_058062.2
RefSeq Size 5360 bp
RefSeq ORF 4158 bp
Locus ID 53321
UniProt ID O54991
Cytogenetics 11 D
MW 156.8 kDa
Summary Required, with CNTNAP2, for radial and longitudinal organization of myelinated axons (PubMed:25378149). Plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Demarcates the paranodal region of the axo-glial junction. In association with contactin involved in the signaling between axons and myelinating glial cells (PubMed:25378149, PubMed:11395000).UniProtKB/Swiss-Prot Function
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Documents
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"NM_016782" in other vectors (4)
SKU Description Size Price
MC224371 Cntnap1 (untagged) - Mouse contactin associated protein-like 1 (Cntnap1), (10ug) 10 ug
$1,304.00
MG223061 Cntnap1 (tGFP-tagged) - Mouse contactin associated protein-like 1 (Cntnap1), (10ug) 10 ug
$1,385.00
MR223061L3 Lenti ORF clone of Cntnap1 (Myc-DDK-tagged) - Mouse contactin associated protein-like 1 (Cntnap1) 10 ug
$1,485.00
MR223061L4 Lenti ORF clone of Cntnap1 (mGFP-tagged) - Mouse contactin associated protein-like 1 (Cntnap1) 10 ug
$1,485.00

Plasmid Map for MR223061

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR223061
Circular map for MR223061

file-alt Citations

Mouse models of human CNTNAP1-associated congenital hypomyelinating neuropathy and genetic restoration of murine neurological deficits
Chang, Sell, Shi et al
Cell Rep (2023) 42 (10), 113274 DOI: 10.1016/j.celrep.2023.113274
Cited by 15
AI Summary: OriGene’s human Cntnap1 expression construct (pCMV-Cntnap1FLAG [MR223061]) was used as the template to generate mutant Cntnap1 constructs for functional studies in this paper.
Snippet: Next, we obtained human Cntnap1 expression construct (pCMV-Cntnap1FLAG [MR223061]) from OriGene and generated independent constructs of Cntnap1C324R and Cntnap1R765C mutations using site-directed mutagenesis
Showing 1-1 of 1 papers.
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