Cntnap1 (NM_016782) Mouse Tagged Lenti ORF Clone

  • LentiORF®
SKU
MR223061L4
Lenti ORF clone of Cntnap1 (mGFP-tagged) - Mouse contactin associated protein-like 1 (Cntnap1)
  $1,485.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cntnap1
Synonyms AI841080; Caspr; NCP1; Nrxn4; p190; shm
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR223061).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016782
ORF Size 4155 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016782.2, NP_058062.2
RefSeq Size 5360 bp
RefSeq ORF 4158 bp
Locus ID 53321
UniProt ID O54991
Cytogenetics 11 D
Summary Required, with CNTNAP2, for radial and longitudinal organization of myelinated axons (PubMed:25378149). Plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Demarcates the paranodal region of the axo-glial junction. In association with contactin involved in the signaling between axons and myelinating glial cells (PubMed:25378149, PubMed:11395000).UniProtKB/Swiss-Prot Function
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"NM_016782" in other vectors (4)
SKU Description Size Price
MC224371 Cntnap1 (untagged) - Mouse contactin associated protein-like 1 (Cntnap1), (10ug) 10 ug
$1,304.00
MG223061 Cntnap1 (tGFP-tagged) - Mouse contactin associated protein-like 1 (Cntnap1), (10ug) 10 ug
$1,385.00
MR223061 Cntnap1 (Myc-DDK-tagged) - Mouse contactin associated protein-like 1 (Cntnap1) 10 ug
$589.00 $1,185.00
MR223061L3 Lenti ORF clone of Cntnap1 (Myc-DDK-tagged) - Mouse contactin associated protein-like 1 (Cntnap1) 10 ug
$1,485.00

Plasmid Map for MR223061L4

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Cloning scheme for MR223061L4

Circular map for MR223061L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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