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Dmc1 (NM_010059) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR222831
Dmc1 (Myc-DDK-tagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
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Other products for "Dmc1 or NM_010059"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dmc1
Synonyms Dmc1h; Lim15; Mei1; Mei11; sg; sgdp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222831 representing NM_010059
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGAGGATCAAGTTGTGCAGGAAGAATCGGGATTCCAAGATGATGAGGAATCTCTGTTTCAAGACA
TTGACCTGTTGCAGAAGCATGGGATTAACATGGCTGATATTAAGAAACTGAAGTCTGTAGGAATCTGTAC
CATTAAAGGGATACAAATGACAACAAGAAGAGCCCTCTGCAATGTCAAAGGCCTCTCAGAAGCCAAAGTG
GAAAAGATTAAAGAGGCAGCCAACAAGCTTATTGAACCAGGATTCCTAACTGCATTTCAGTACAGTGAAA
GGAGGAAGATGGTATTCCATATCACTACTGGGAGCCAGGAATTTGATAAATTACTAGGAGGTGGCATTGA
AAGTATGGCAATTACAGAAGCTTTTGGAGAATTTCGTACTGGAAAAACTCAGCTGTCTCATACCCTCTGT
GTGACAGCTCAACTTCCAGGAACAGGCGGCTACTCAGGTGGAAAGATTATCTTCATCGATACAGAAAATA
CTTTCCGTCCAGATCGCCTTCGGGACATTGCTGACCGCTTCAACGTAGACCATGAAGCAGTACTGGACAA
CGTGCTCTATGCACGTGCCTATACTAGTGAGCATCAGATGGAGCTACTTGATTATGTCGCAGCCAAGTTT
CATGAGGAAGCTGGCATTTTCAAACTATTGATCATTGACTCAATAATGGCACTTTTTCGAGTGGATTTCA
GTGGCCGTGGGGAATTGGCTGAACGCCAGCAAAAATTGGCCCAGATGTTGTCACGACTCCAAAAAATCTC
AGAAGAATATAATGTTGCTGTTTTTGTGACCAATCAAATGACTGCAGATCCAGGAGCAACTATGACCTTT
CAGGCTGATCCCAAAAAACCCATTGGTGGACACATTCTGGCTCACGCTTCCACAACAAGAATAAGCTTGC
GCAAGGGAAGAGGAGAACTGAGGATCGCCAAGATTTATGACAGCCCCGAGATGCCTGAAAATGAAGCCAC
CTTCGCAATAACCGCTGGAGGGATCGGGGATGCCAAGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222831 representing NM_010059
Red=Cloning site Green=Tags(s)

MKEDQVVQEESGFQDDEESLFQDIDLLQKHGINMADIKKLKSVGICTIKGIQMTTRRALCNVKGLSEAKV
EKIKEAANKLIEPGFLTAFQYSERRKMVFHITTGSQEFDKLLGGGIESMAITEAFGEFRTGKTQLSHTLC
VTAQLPGTGGYSGGKIIFIDTENTFRPDRLRDIADRFNVDHEAVLDNVLYARAYTSEHQMELLDYVAAKF
HEEAGIFKLLIIDSIMALFRVDFSGRGELAERQQKLAQMLSRLQKISEEYNVAVFVTNQMTADPGATMTF
QADPKKPIGGHILAHASTTRISLRKGRGELRIAKIYDSPEMPENEATFAITAGGIGDAKE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010059
ORF Size 1020 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010059.3, NP_034189.1
RefSeq Size 2227 bp
RefSeq ORF 1023 bp
Locus ID 13404
UniProt ID Q61880
Cytogenetics 15 37.79 cM
MW 37.8 kDa
Summary This gene encodes a member of the superfamily of recombinases (also called DNA strand-exchange proteins). Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. This protein, which is evolutionarily conserved, is reported to be essential for meiotic homologous recombination and may thus play an important role in generating diversity of genetic information. In mouse, deficiency of this gene causes infertility. Alternative splicing results in multiple transcript variants. provided by RefSeq, May 2013
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Documents
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"NM_010059" in other vectors (4)
SKU Description Size Price
MC208400 Dmc1 (untagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1), (10ug) 10 ug
$503.00
MG222831 Dmc1 (tGFP-tagged) - Mouse DMC1 dosage suppressor of mck1 homolog meiosis-specific homologous recombination (yeast) (Dmc1), (10ug) 10 ug
$657.00
MR222831L3 Lenti ORF clone of Dmc1 (Myc-DDK-tagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1) 10 ug
$757.00
MR222831L4 Lenti ORF clone of Dmc1 (mGFP-tagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1) 10 ug
$757.00

Plasmid Map for MR222831

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222831
Circular map for MR222831

file-alt Citations

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