Dmc1 (NM_010059) Mouse Untagged Clone

SKU
MC208400
Dmc1 (untagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1), (10ug)
  $503.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dmc1
Synonyms Dmc1h; Lim15; Mei1; Mei11; sg; sgdp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC208400 representing NM_010059
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGAGGATCAAGTTGTGCAGGAAGAATCGGGATTCCAAGATGATGAGGAATCTCTGTTTCAAGACA
TTGACCTGTTGCAGAAGCATGGGATTAACATGGCTGATATTAAGAAACTGAAGTCTGTAGGAATCTGTAC
CATTAAAGGGATACAAATGACAACAAGAAGAGCCCTCTGCAATGTCAAAGGCCTCTCAGAAGCCAAAGTG
GAAAAGATTAAAGAGGCAGCCAACAAGCTTATTGAACCAGGATTCCTAACTGCATTTCAGTACAGTGAAA
GGAGGAAGATGGTATTCCATATCACTACTGGGAGCCAGGAATTTGATAAATTACTAGGAGGTGGCATTGA
AAGTATGGCAATTACAGAAGCTTTTGGAGAATTTCGTACTGGAAAAACTCAGCTGTCTCATACCCTCTGT
GTGACAGCTCAACTTCCAGGAACAGGCGGCTACTCAGGTGGAAAGATTATCTTCATCGATACAGAAAATA
CTTTCCGTCCAGATCGCCTTCGGGACATTGCTGACCGCTTCAACGTAGACCATGAAGCAGTACTGGACAA
CGTGCTCTATGCACGTGCCTATACTAGTGAGCATCAGATGGAGCTACTTGATTATGTCGCAGCCAAGTTT
CATGAGGAAGCTGGCATTTTCAAACTATTGATCATTGACTCAATAATGGCACTTTTTCGAGTGGATTTCA
GTGGCCGTGGGGAATTGGCTGAACGCCAGCAAAAATTGGCCCAGATGTTGTCACGACTCCAAAAAATCTC
AGAAGAATATAATGTTGCTGTTTTTGTGACCAATCAAATGACTGCAGATCCAGGAGCAACTATGACCTTT
CAGGCTGATCCCAAAAAACCCATTGGTGGACACATTCTGGCTCACGCTTCCACAACAAGAATAAGCTTGC
GCAAGGGAAGAGGAGAACTGAGGATCGCCAAGATTTATGACAGCCCCGAGATGCCTGAAAATGAAGCCAC
CTTCGCAATAACCGCTGGAGGGATCGGGGATGCCAAGGAGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_010059
Insert Size 1023 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010059.3, NP_034189.1
RefSeq Size 2227 bp
RefSeq ORF 1023 bp
Locus ID 13404
UniProt ID Q61880
Cytogenetics 15 37.79 cM
Summary This gene encodes a member of the superfamily of recombinases (also called DNA strand-exchange proteins). Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. This protein, which is evolutionarily conserved, is reported to be essential for meiotic homologous recombination and may thus play an important role in generating diversity of genetic information. In mouse, deficiency of this gene causes infertility. Alternative splicing results in multiple transcript variants. provided by RefSeq, May 2013
Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_010059" in other vectors (4)
SKU Description Size Price
MG222831 Dmc1 (tGFP-tagged) - Mouse DMC1 dosage suppressor of mck1 homolog meiosis-specific homologous recombination (yeast) (Dmc1), (10ug) 10 ug
$657.00
MR222831 Dmc1 (Myc-DDK-tagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1) 10 ug
$289.00 $457.00
MR222831L3 Lenti ORF clone of Dmc1 (Myc-DDK-tagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1) 10 ug
$757.00
MR222831L4 Lenti ORF clone of Dmc1 (mGFP-tagged) - Mouse DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast) (Dmc1) 10 ug
$757.00

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