OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Cst7 (NM_009977) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR222507
Cst7 (Myc-DDK-tagged) - Mouse cystatin F (leukocystatin) (Cst7)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Cst7 or NM_009977"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cst7
Synonyms Cmap
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR222507 representing NM_009977
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTGGTCCTGGAGCTGTACTTGCCGAGCACTTGGCCCACTACATGCTCCCTGCATTTCCCCAGCCA
TGTGGCTGGCCATTCTGCTTGCCCTCTGCTGCCTAACTTCTGACACCCATGGGGCACGCCCCCCAGATTT
TTGTTCCAAAGATTTGATCTCCAGTGTGAAGCCAGGATTCCCCAAAACAATAGAGACCAATAACCCAGGA
GTGCTTAAGGCCGCCAGGCACAGTGTGGAAAAGTTCAACAACTGCACAAATGACATCTTTTTGTTCAAGG
AGTCCCATGTCAGCAAAGCCCTGGTACAGGTGGTGAAAGGCCTGAAATATATGCTGGAGGTGAAAATCGG
CCGAACTACATGCAGGAAGACCATGCATCACCAACTGGACAACTGTGACTTCCAAACCAACCCTGCCTTG
AAGCGGACTCTATATTGCTACTCTGAAGTCTGGGTCATCCCCTGGCTCCACAGTTTTGAGGTGCCTGTTC
TCCTCTGCCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR222507 representing NM_009977
Red=Cloning site Green=Tags(s)

MPWSWSCTCRALGPLHAPCISPAMWLAILLALCCLTSDTHGARPPDFCSKDLISSVKPGFPKTIETNNPG
VLKAARHSVEKFNNCTNDIFLFKESHVSKALVQVVKGLKYMLEVKIGRTTCRKTMHHQLDNCDFQTNPAL
KRTLYCYSEVWVIPWLHSFEVPVLLCQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009977
ORF Size 501 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009977.2
RefSeq Size 986 bp
RefSeq ORF 435 bp
Locus ID 13011
UniProt ID O89098
Cytogenetics 2 G3
MW 16.8 kDa
Summary Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_009977" in other vectors (4)
SKU Description Size Price
MC208350 Cst7 (untagged) - Mouse cystatin F (leukocystatin) (Cst7), (10ug) 10 ug
$165.00
MG222507 Cst7 (tGFP-tagged) - Mouse cystatin F (leukocystatin) (Cst7), (10ug) 10 ug
$500.00
MR222507L3 Lenti ORF clone of Cst7 (Myc-DDK-tagged) - Mouse cystatin F (leukocystatin) (Cst7) 10 ug
$600.00
MR222507L4 Lenti ORF clone of Cst7 (mGFP-tagged) - Mouse cystatin F (leukocystatin) (Cst7) 10 ug
$600.00

Plasmid Map for MR222507

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR222507
Circular map for MR222507

file-alt Citations

Enhanced legumain activity links progranulin deficiency to TDP-43 pathology in frontotemporal lobar degeneration
Robinson, Reich, Mühlhofer et al
bioRxiv (2024)
Cited by 5
Snippet: … Progranulin (PGRN) loss leads to;Mouse cystation-3 (# MR225378, OriGene) and cystatin-7 (# MR222507, OriGene) myc-tagged cDNA clones were subcloned into the BamHI/EcoRI sites of pcDNA3 using the following primers: Cst3 (BamHI) forward 5’-CCCGGATCCCCAACCATGGCCAGCCCGCTGC-3’ Cst7 (BamHI) forward 5’- CCCGGATCCCCAGCCATGCCCTGGTCCTGG -3’
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products