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Cst7 (NM_009977) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG222507
Cst7 (tGFP-tagged) - Mouse cystatin F (leukocystatin) (Cst7), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cst7
Synonyms Cmap
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG222507 representing NM_009977
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTGGTCCTGGAGCTGTACTTGCCGAGCACTTGGCCCACTACATGCTCCCTGCATTTCCCCAGCCA
TGTGGCTGGCCATTCTGCTTGCCCTCTGCTGCCTAACTTCTGACACCCATGGGGCACGCCCCCCAGATTT
TTGTTCCAAAGATTTGATCTCCAGTGTGAAGCCAGGATTCCCCAAAACAATAGAGACCAATAACCCAGGA
GTGCTTAAGGCCGCCAGGCACAGTGTGGAAAAGTTCAACAACTGCACAAATGACATCTTTTTGTTCAAGG
AGTCCCATGTCAGCAAAGCCCTGGTACAGGTGGTGAAAGGCCTGAAATATATGCTGGAGGTGAAAATCGG
CCGAACTACATGCAGGAAGACCATGCATCACCAACTGGACAACTGTGACTTCCAAACCAACCCTGCCTTG
AAGCGGACTCTATATTGCTACTCTGAAGTCTGGGTCATCCCCTGGCTCCACAGTTTTGAGGTGCCTGTTC
TCCTCTGCCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG222507 representing NM_009977
Red=Cloning site Green=Tags(s)

MPWSWSCTCRALGPLHAPCISPAMWLAILLALCCLTSDTHGARPPDFCSKDLISSVKPGFPKTIETNNPG
VLKAARHSVEKFNNCTNDIFLFKESHVSKALVQVVKGLKYMLEVKIGRTTCRKTMHHQLDNCDFQTNPAL
KRTLYCYSEVWVIPWLHSFEVPVLLCQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009977
ORF Size 501 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009977.2
RefSeq Size 986 bp
RefSeq ORF 435 bp
Locus ID 13011
UniProt ID O89098
Cytogenetics 2 G3
Summary Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.UniProtKB/Swiss-Prot Function
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"NM_009977" in other vectors (4)
SKU Description Size Price
MC208350 Cst7 (untagged) - Mouse cystatin F (leukocystatin) (Cst7), (10ug) 10 ug
$165.00
MR222507 Cst7 (Myc-DDK-tagged) - Mouse cystatin F (leukocystatin) (Cst7) 10 ug
$289.00 $300.00
MR222507L3 Lenti ORF clone of Cst7 (Myc-DDK-tagged) - Mouse cystatin F (leukocystatin) (Cst7) 10 ug
$600.00
MR222507L4 Lenti ORF clone of Cst7 (mGFP-tagged) - Mouse cystatin F (leukocystatin) (Cst7) 10 ug
$600.00

Plasmid Map for MG222507

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG222507
Circular map for MG222507

file-alt Citations

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